Comparative Study of the γH2AX Foci Forming in Human Lung Fibroblasts Incubated in Media Containing Tritium-Labeled Thymidine or Amino Acids

2021 ◽  
Vol 172 (2) ◽  
pp. 245-249
Author(s):  
S. M. Rodneva ◽  
A. A. Osipov ◽  
D. V. Guryev ◽  
A. A. Tsishnatti ◽  
Y. А. Fedotov ◽  
...  
Keyword(s):  
Amino Acids ◽  
Comparative Study ◽  
Human Lung ◽  
Lung Fibroblasts ◽  
Human Lung Fibroblasts ◽  
Γh2ax Foci

Sulfation by human lung fibroblasts: SO4(2-) and sulfur-containing amino acids as sources for macromolecular sulfation

1991 ◽  
Vol 260 (6) ◽  
pp. L450-L456 ◽  
Author(s):  
A. Elgavish ◽  
E. Meezan
Keyword(s):  
Amino Acids ◽  
Plasma Membrane ◽  
Human Lung ◽  
Lung Fibroblasts ◽  
Human Lung Fibroblasts ◽  
Physiological Conditions ◽  
Limiting Step ◽  
Sulfate Incorporation ◽  
Sulfur Containing ◽  
Inorganic Sulfate

Studies were carried out in human lung fibroblasts (IMR-90) to investigate 1) the relative contribution of two extracellular pools, inorganic sulfate and sulfur-containing amino acids, to the intracellular fraction precipitable by trichloroacetic acid and 2) the possibility that the transport of these sulfur-containing substrates at the plasma membrane may be a limiting step for macromolecular sulfation. Our studies indicate that the ability to use SO4(2-) released by intracellular catabolism of the sulfur-containing amino acid L-cysteine differs from one cell system to another. In contrast to smooth muscle cells, in the human lung fibroblast, L-cysteine contributes significantly to the intercellular pool of SO4(2-) used for sulfation at extracellular [SO4(2-)] less than 100 microM. However, under physiological conditions with respect to SO4(2-) ([SO4(2-)]0 = 300 microM), L-cysteine does not contribute greater than 30% of the sulfate incorporated into the cellular fraction. Taurine (2-aminoethanesulfonic acid) inhibits SO4(2-) incorporation into the cell-associated macromolecular fraction. However, results suggest that the effect is not due to either SO4(2-) released by its catabolism or to an effect on SO4(2-) transport into the cell. The fact that the transport inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid inhibits sulfate incorporation indicates that carrier-mediated sulfate transport at the cellular plasma membrane may be a limiting step for sulfate incorporation. In conclusion, under physiological conditions with respect to SO4(2-), inorganic sulfate is a major source of sulfate for sulfation in human lung fibroblasts and macromolecular sulfation may be limited by its transport into the cells.

scholarly journals Fenofibrate inhibits TGF‐β‐induced myofibroblast differentiation and activation in human lung fibroblasts in vitro

FEBS Open Bio ◽  
2021 ◽  
Author(s):  
Ryota Kikuchi ◽  
Yuki Maeda ◽  
Takao Tsuji ◽  
Kazuhiro Yamaguchi ◽  
Shinji Abe ◽  
...  
Keyword(s):  
Human Lung ◽  
Lung Fibroblasts ◽  
Myofibroblast Differentiation ◽  
Human Lung Fibroblasts

scholarly journals Procollagen production and procollagen messenger RNA levels and activity in human lung fibroblasts during periods of rapid and stationary growth.

1981 ◽  
Vol 256 (6) ◽  
pp. 3135-3140
Author(s):  
P. Tolstoshev ◽  
R.A. Berg ◽  
S.I. Rennard ◽  
K.H. Bradley ◽  
B.C. Trapnell ◽  
...  
Keyword(s):  
Messenger Rna ◽  
Human Lung ◽  
Lung Fibroblasts ◽  
Stationary Growth ◽  
Human Lung Fibroblasts ◽  
Rna Levels
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