Competition is the mechanism of biocontrol of brown rot in stone fruit by Penicillium frequentans

BioControl ◽  
2017 ◽  
Vol 62 (4) ◽  
pp. 557-566 ◽  
Author(s):  
Belen Guijarro ◽  
Laura Hernandez-Escribano ◽  
Inmaculada Larena ◽  
Paloma Melgarejo ◽  
Antonieta De Cal
Keyword(s):  
Brown Rot ◽  
Stone Fruit ◽  
Penicillium Frequentans

scholarly journals Deciphering the Monilinia fructicola Genome to Discover Effector Genes Possibly Involved in Virulence

Genes ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 568
Author(s):  
Laura Vilanova ◽  
Claudio A. Valero-Jiménez ◽  
Jan A.L. van Kan
Keyword(s):  
Brown Rot ◽  
Biological Properties ◽  
Effector Proteins ◽  
Stone Fruit ◽  
Rna Seq ◽  
Sequencing Technology ◽  
Pathogen Virulence ◽  
Effector Genes ◽  
Gene Models ◽  
High Quality Genome

Brown rot is the most economically important fungal disease of stone fruits and is primarily caused by Monilinia laxa and Monlinia fructicola. Both species co-occur in European orchards although M. fructicola is considered to cause the most severe yield losses in stone fruit. This study aimed to generate a high-quality genome of M. fructicola and to exploit it to identify genes that may contribute to pathogen virulence. PacBio sequencing technology was used to assemble the genome of M. fructicola. Manual structural curation of gene models, supported by RNA-Seq, and functional annotation of the proteome yielded 10,086 trustworthy gene models. The genome was examined for the presence of genes that encode secreted proteins and more specifically effector proteins. A set of 134 putative effectors was defined. Several effector genes were cloned into Agrobacterium tumefaciens for transient expression in Nicotiana benthamiana plants, and some of them triggered necrotic lesions. Studying effectors and their biological properties will help to better understand the interaction between M. fructicola and its stone fruit host plants.

scholarly journals Identification and Characterization of Benzimidazole Resistance in Monilinia fructicola from Stone Fruit Orchards in California

2003 ◽  
Vol 69 (12) ◽  
pp. 7145-7152 ◽  
Author(s):  
Zhonghua Ma ◽  
Michael A. Yoshimura ◽  
Themis J. Michailides
Keyword(s):  
Dna Sequences ◽  
Point Mutations ◽  
Brown Rot ◽  
Stone Fruit ◽  
Monilinia Fructicola ◽  
Tubulin Gene ◽  
Benzimidazole Fungicides ◽  
Specific Pcr ◽  
Allele Specific ◽  
Fruit Orchards

ABSTRACT Low and high levels of resistance to the benzimidazole fungicides benomyl and thiophanate-methyl were observed in field isolates of Monilinia fructicola, which is the causative agent of brown rot of stone fruit. Isolates that had low levels of resistance (hereafter referred to as LR isolates) and high levels of resistance (hereafter referred to as HR isolates) were also cold and heat sensitive, respectively. Results from microsatellite DNA fingerprints showed that genetic identities among the populations of sensitive (S), LR, and HR isolates were very high (>0.96). Analysis of DNA sequences of theβ -tubulin gene showed that the LR isolates had a point mutation at codon 6, causing a replacement of the amino acid histidine by tyrosine. Codon 198, which encodes a glutamic acid in S and LR isolates, was converted to a codon for alanine in HR isolates. Based on these point mutations in the β-tubulin gene, allele-specific PCR assays were developed for rapid detection of benzimidazole-resistant isolates of M. fructicola from stone fruit.

Radio Frequency Treatment to Control Postharvest Brown Rot in Stone Fruit

2014 ◽  
pp. 89-99
Author(s):  
M. Sisquella ◽  
C. Casals ◽  
P. Picouet ◽  
I. Viñas ◽  
R. Torres ◽  
...  
Keyword(s):  
Radio Frequency ◽  
Brown Rot ◽  
Stone Fruit

Dispersion, persistence, and stability of the biocontrol agent Penicillium frequentans strain 909 after stone fruit tree applications

2019 ◽  
Vol 26 (28) ◽  
pp. 29138-29156 ◽  
Author(s):  
Belen Guijarro ◽  
Inmaculada Larena ◽  
Laura Vilanova ◽  
Rosario Torres ◽  
Marta Balsells-Llauradó ◽  
...  
Keyword(s):  
Biocontrol Agent ◽  
Fruit Tree ◽  
Stone Fruit ◽  
Penicillium Frequentans ◽  
Stone Fruit Tree

scholarly journals Population dynamics ofEpicoccum nigrum, a biocontrol agent against brown rot in stone fruit

2009 ◽  
Vol 106 (2) ◽  
pp. 592-605 ◽  
Author(s):  
A. De Cal ◽  
I. Larena ◽  
M. Liñán ◽  
R. Torres ◽  
N. Lamarca ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Biocontrol Agent ◽  
Brown Rot ◽  
Stone Fruit

Improvement of microwave treatment with immersion of fruit in water to control brown rot in stone fruit

2014 ◽  
Vol 26 ◽  
pp. 168-175 ◽  
Author(s):  
María Sisquella ◽  
Pierre Picouet ◽  
Inmaculada Viñas ◽  
Neus Teixidó ◽  
Joan Segarra ◽  
...  
Keyword(s):  
Brown Rot ◽  
Microwave Treatment ◽  
Stone Fruit

scholarly journals Detection of Latent Monilinia Infections in Nectarine Flowers and Fruit by qPCR

Plant Disease ◽  
2017 ◽  
Vol 101 (6) ◽  
pp. 1002-1008 ◽  
Author(s):  
C. Garcia-Benitez ◽  
P. Melgarejo ◽  
A. De Cal
Keyword(s):  
Prunus Persica ◽  
Brown Rot ◽  
Stone Fruit ◽  
Monilinia Fructicola ◽  
Latent Infections ◽  
Established Method ◽  
Latently Infected ◽  
Dna Amounts

Most stone fruit with a latent brown rot infection caused by Monilinia do not develop visible signs of disease until the arrival of fruit at the markets or the consumer’s homes. The overnight freezing-incubation technique (ONFIT) is a well-established method for detecting latent brown rot infections, but it takes between 7 to 9 days. In this report, we inform on the advantages of applying a qPCR-based method to (i) detect a latent brown rot infection in the blossoms and fruit of nectarine trees (Prunus persica var. nucipersica) and (ii) distinguish between the Monilinia spp. in them. For applying this qPCR-based method, artificial latent infections were established in nectarine flowers and fruit using 10 Monilinia fructicola isolates, 8 M. fructigena isolates, and 10 M. laxa isolates. We detected greater amounts of M. fructicola DNA than M. laxa and M. fructigena DNA in latently infected flowers using qPCR. However, greater DNA amounts of M. laxa than M. fructicola were detected in the mesocarp of latently infected nectarines. We found that the qPCR-based method is more sensitive, reliable, and quicker than ONFIT for detecting a latent brown rot infection, and could be very useful in those countries where Monilinia spp. are classified as quarantine pathogens.

scholarly journals Monilinia spp. Causing Brown Rot of Stone Fruit in Serbia

Plant Disease ◽  
2015 ◽  
Vol 99 (5) ◽  
pp. 709-717 ◽  
Author(s):  
Jovana Hrustić ◽  
Goran Delibašić ◽  
Ivana Stanković ◽  
Mila Grahovac ◽  
Branka Krstić ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Brown Rot ◽  
Morphological Characterization ◽  
Stone Fruit ◽  
Monilinia Laxa ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Rot Disease ◽  
First Time

Brown rot is one of the most important pre- and postharvest fungal diseases of stone fruit worldwide. In Serbia, where production of stone fruit is economically important, Monilinia laxa and M. fructigena are widely distributed. In surveys from 2011 to 2013, 288 isolates of Monilinia spp. were collected from 131 localities in 16 districts and from six hosts in Serbia. Using multiplex polymerase chain reaction, phylogenetic analysis, and morphological characterization, three species of Monilinia were identified as the causal agents of brown rot of stone fruit: M. laxa (89% of isolates), M. fructigena (3%), and M. fructicola (8%). In 2011, M. fructicola was reported for the first time on stone fruit in Serbia, with only one isolate detected. More isolates of M. fructicola were detected in 2012 (2 isolates) and 2013 (20 isolates). The presence of M. fructicola, as well as its increased frequency of detection during the survey, may indicate a change in the population structure of these pathogens, which could have an important impact on brown rot disease management in Serbia.

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