Monilinia spp. Causing Brown Rot of Stone Fruit in Serbia

Brown rot is one of the most important pre- and postharvest fungal diseases of stone fruit worldwide. In Serbia, where production of stone fruit is economically important, Monilinia laxa and M. fructigena are widely distributed. In surveys from 2011 to 2013, 288 isolates of Monilinia spp. were collected from 131 localities in 16 districts and from six hosts in Serbia. Using multiplex polymerase chain reaction, phylogenetic analysis, and morphological characterization, three species of Monilinia were identified as the causal agents of brown rot of stone fruit: M. laxa (89% of isolates), M. fructigena (3%), and M. fructicola (8%). In 2011, M. fructicola was reported for the first time on stone fruit in Serbia, with only one isolate detected. More isolates of M. fructicola were detected in 2012 (2 isolates) and 2013 (20 isolates). The presence of M. fructicola, as well as its increased frequency of detection during the survey, may indicate a change in the population structure of these pathogens, which could have an important impact on brown rot disease management in Serbia.

Download Full-text

Multiplex Polymerase Chain Reaction Analysis of UV-A– and UV-B–induced Delayed and Early Mutations in V79 Chinese Hamster Cells¶

Photochemistry and Photobiology ◽

10.1562/2004-05-19-ra-174.1 ◽

2005 ◽

Vol 81 (1) ◽

pp. 114 ◽

Cited By ~ 7

Author(s):

Jostein Dahle ◽

Paul Noordhuis ◽

Trond Stokke ◽

Debbie Hege Svendsrud ◽

Egil Kvam

Keyword(s):

Polymerase Chain Reaction ◽

Multiplex Polymerase Chain Reaction ◽

Chinese Hamster ◽

Polymerase Chain Reaction Analysis ◽

Chain Reaction ◽

Chinese Hamster Cells ◽

Polymerase Chain

Download Full-text

Relationship Between the para-Homologous Sodium Channel Point Mutation (g → c at Nucleotide 2979) and Knockdown Resistance in the German Cockroach Using Multiplex Polymerase Chain Reaction to Discern Genotype

Journal of Economic Entomology ◽

10.1603/0022-0493-96.3.885 ◽

2003 ◽

Vol 96 (3) ◽

pp. 885-891 ◽

Cited By ~ 1

Author(s):

Steven M. Valles ◽

Omaththage P. Perera ◽

Charles A. Strong

Keyword(s):

Polymerase Chain Reaction ◽

Sodium Channel ◽

Point Mutation ◽

Multiplex Polymerase Chain Reaction ◽

German Cockroach ◽

Knockdown Resistance ◽

Chain Reaction ◽

Polymerase Chain

Download Full-text

Virus respiratoires dans les pneumonies associées aux soins

Médecine Intensive Réanimation ◽

10.3166/rea-2018-0049 ◽

2018 ◽

Vol 27 (3) ◽

pp. 217-227

Author(s):

P. Loubet ◽

G. Voiriot ◽

M. Neuville ◽

B. Visseaux ◽

J.-F. Timsit

Keyword(s):

Polymerase Chain Reaction ◽

Multiplex Polymerase Chain Reaction ◽

Chain Reaction ◽

Biologie Moléculaire ◽

Mise En Œuvre ◽

Polymerase Chain ◽

Infection Bactérienne

Les pneumonies acquises à l’hôpital (PAH) sont fréquentes. À l’ère des techniques diagnostiques de biologie moléculaire (multiplex polymerase chain reaction), les rares données disponibles estiment que les virus respiratoires sont impliqués dans 22 à 32 % des épisodes. Les patients immunodéprimés constituent probablement la population la plus à risque. La présentation clinique et radiologique ne diffère pas entre pneumonies bactériennes, virales et mixtes (virus–bactérie). L’excrétion prolongée de virus respiratoires dans les voies aériennes a été rapportée chez les patients immunodéprimés. Elle pourrait promouvoir la co-infection bactérienne, associée à des durées d’hospitalisation prolongées. L’acquisition intrahospitalière a été démontrée chez tous les virus respiratoires. Elle encourage la mise en œuvre et le respect des mesures d’hygiène et de confinement, dans l’objectif de protéger soignants, visiteurs et patients. De nombreux points restent largement méconnus, relatifs aux interactions entre virus respiratoires et pathogènes non viraux, aux périodes d’incubation, ou encore aux durées d’excrétion virale. L’amélioration des techniques diagnostiques et l’accumulation de données épidémiologiques et cliniques devraient permettre de mieux appréhender le rôle des virus respiratoires dans les PAH. Cette meilleure connaissance aidera à rationaliser l’utilisation des tests de détection et facilitera l’interprétation de leurs résultats. Elle guidera aussi le clinicien dans l’utilisation future des nombreuses molécules antivirales actuellement en développement clinique chez l’homme.

Download Full-text

Molecular Analysis of Uropathogenic E.coli Isolates from Urinary Tract Infections

Infectious Disorders - Drug Targets ◽

10.2174/1871526518666181113091322 ◽

2019 ◽

Vol 19 (3) ◽

pp. 322-326 ◽

Cited By ~ 1

Author(s):

Hassan Valadbeigi ◽

Elham Esmaeeli ◽

Sobhan Ghafourian ◽

Abbas Maleki ◽

Nourkhoda Sadeghifard

Keyword(s):

Escherichia Coli ◽

Polymerase Chain Reaction ◽

Urinary Tract ◽

Urinary Tract Infections ◽

Multiplex Polymerase Chain Reaction ◽

Virulence Genes ◽

Uropathogenic Escherichia Coli ◽

Chain Reaction ◽

Polymerase Chain ◽

Tract Infections

Introduction: The aim of the current study was to investigate the prevalence of virulence genes in uropathogenic Escherichia coli (UPEC) isolates in Ilam. Materials and Methods: For this purpose, a total of 80 UPEC isolates were collected for patients with UTIs during a 6 months period. The multiplex polymerase chain reaction (multiplex PCR) was used to detect the papEF, fimH, iucD, hlyA, fyuA, and ompT genes. Results: The prevalence of fimH, papEF, iucD, fyuA, hlyA, hlyA, and ompT genes were 87.5%, 47.5%, 60%, 67.5%, 27.5%, 47.5% and 71.2%, respectively. Among all of the isolates, 27 profiles were obtained. Conclusion: Our findings demonstrated that the most prevalence was found for fimH, and different distribution of virulence genes suggested different ability of pathogenicity.

Download Full-text