Methyl jasmonate mediates upregulation of bacoside A production in shoot cultures of Bacopa monnieri

Bacopa monnieri has been used as a reputed drug in the Indian traditional ayurvedic system for centuries. This medicinal herb with important phytopharmaceuticals has been popularly known as “Brahmi”. In recent years, B. monnieri has been extensively studied for its bioactive constituents, constituents responsible for memory enhancing effect, and also its diverse other useful effects. It possesses many pharmacological activities such as antioxidant, gastrointestinal, endocrine, antimicrobial, anti-inflammatory etc. The plant has been also used for the treatment of neurological and neuropsychiatric diseases. Due to its multipurpose therapeutic potential, micropropagation using axillary meristems and de novo organogenesis has been extensively studied in the species and is being reviewed. High frequency direct shoot organogenesis can be induced in excised leaf and internode explants in the absence of exogenous phytohormones and the rate of induction is enhanced in the presence of exogenous cytokinins, supplements, growth regulators, etc. Using explants from tissue culture raised plants, direct shoot regeneration leading to production of more than 100 rooted plants/explant within 8–12 weeks period with 85%–100% survival in the field after acclimatization can be expected following optimized protocols. Bioreactor based micropropagation was found to increase the multiplication rate of shoot cultures for the commercial propagation of B. monnieri plants. The maximum content of bacosides has been recorded in shoot biomass using an airlift bioreactor system. Further studies for the biosynthesis of bacosides and other secondary metabolites need to be conducted in the species utilizing untransformed shoot cultures in bioreactors.

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Statistical optimization for enhanced bacoside A production in plant cell cultures of Bacopa monnieri

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-017-1373-6 ◽

2017 ◽

Vol 133 (2) ◽

pp. 203-214 ◽

Cited By ~ 6

Author(s):

J. Leonard ◽

Bishwanath Seth ◽

Binod B. Sahu ◽

V. R. Singh ◽

Nivedita Patra

Keyword(s):

Plant Cell ◽

Cell Cultures ◽

Bacopa Monnieri ◽

Statistical Optimization ◽

Plant Cell Cultures ◽

Bacoside A

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Analysis of nuclear DNA content, genetic stability, Bacoside A quantity and antioxidant potential of long term in vitro grown germplasm lines of Bacopa monnieri (L.)

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-014-0602-5 ◽

2014 ◽

Vol 120 (1) ◽

pp. 399-406 ◽

Cited By ~ 5

Author(s):

Muthiah Joe Virgin Largia ◽

Jayabalan Shilpha ◽

Govindan Pothiraj ◽

Manikandan Ramesh

Keyword(s):

Dna Content ◽

Genetic Stability ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Bacopa Monnieri ◽

Antioxidant Potential ◽

Bacoside A

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Elite genotypes of Bacopa monnieri , with high contents of Bacoside A and Bacopaside I, from southern Western Ghats in India

Industrial Crops and Products ◽

10.1016/j.indcrop.2017.01.018 ◽

2017 ◽

Vol 98 ◽

pp. 76-81 ◽

Cited By ~ 5

Author(s):

Cinthya Christopher ◽

Anil John Johnson ◽

Paravanparampil Jacob Mathew ◽

Sabulal Baby

Keyword(s):

Western Ghats ◽

Bacopa Monnieri ◽

Bacoside A ◽

Southern Western Ghats

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Development and Validation of a Novel, Rapid Gradient HPLC Method for Simultaneous Estimation of Bioactive Marker Compounds in a Mixture of Convolvulus pluricaulis, Withania somnifera and Bacopa monnieri Extracts

Journal of Chromatographic Science ◽

10.1093/chromsci/bmz075 ◽

2019 ◽

Vol 57 (10) ◽

pp. 920-930 ◽

Cited By ~ 1

Author(s):

Sunayna Choudhary ◽

Indu Pal Kaur ◽

Jai Malik

Keyword(s):

Withania Somnifera ◽

Therapeutic Option ◽

Herbal Medicines ◽

Hplc Method ◽

Bacopa Monnieri ◽

Withaferin A ◽

Simultaneous Estimation ◽

Bacoside A ◽

Convolvulus Pluricaulis ◽

Development And Validation

Abstract The use of herbs as medicine is an ancient form of healthcare known to mankind. Standardization of herbal medicines is however a challenging task and is the major bottleneck in their acceptance as the primary therapeutic option. The aim of this study was to develop and validate a simple, rapid HPLC method for standardizing the mixture of extracts of three Medhya Rasayanas (neurotonic), Convolvulus pluricaulis, Withania somnifera and Bacopa monnieri. Simultaneous estimation of the respective bioactive markers of these plants viz., scopoletin, withaferin A, bacoside A 3, bacopaside II, jujubogenin and bacosaponin C has been reported for the first time. The method was developed using Waters Hybrid X-Bridge shield with BEH technology 2.5 μm, 4.6 × 75 mm column and validated according to ICH guidelines. The 20 minutes run time makes the method eco-friendly. The method was linear over a range of 12.5–400 ng/10 μL for scopoletin and 62.5–2,000 ng/10 μL for withaferin A, bacoside A 3, bacopaside II, jujubogenin and bacosaponin C with detection limits of 8.0, 48.3, 30.4, 40.7, 15.6 and 18.9 ng/10 μL and quantification limits of 24.5, 146.5, 92.2, 123.4, 47.4 and 57.4 ng/10 μL, respectively. The correlation coefficient for each analyte was >0.999. The intra-day and inter-day precision was <2%. These results confirmed the precision, accuracy and robustness of the proposed method.

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