scholarly journals Influence of different media, medium strength and carbon sources on adventitious shoot cultures and production of bacoside A in Bacopa monnieri(L.)

2017 ◽  
Vol 46 (4) ◽  
pp. 97 ◽  
Author(s):  
Poornananda Madhava Naik ◽  
Manoj Godbole ◽  
Praveen Nagella ◽  
Hosakatte Niranjana Murthy
Keyword(s):  
Carbon Sources ◽  
Adventitious Shoot ◽  
Bacopa Monnieri ◽  
Shoot Cultures ◽  
Bacoside A ◽  
Medium Strength

Methyl jasmonate mediates upregulation of bacoside A production in shoot cultures of Bacopa monnieri

2013 ◽  
Vol 35 (7) ◽  
pp. 1121-1125 ◽  
Author(s):  
Poojadevi Sharma ◽  
Sheetal Yadav ◽  
Anshu Srivastava ◽  
Neeta Shrivastava
Keyword(s):  
Methyl Jasmonate ◽  
Bacopa Monnieri ◽  
Shoot Cultures ◽  
Bacoside A

The Effect of Heavy Metals on In Vitro Adventitious Shoot Production and Bacoside A Content in Bacopa Monnieri (L)

2017 ◽  
Vol 14 (4) ◽  
pp. 1-10
Author(s):  
Poornananda Madhava Naik Manoj Godbole
Keyword(s):  
Heavy Metals ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Dry Weight ◽  
Bacopa Monnieri ◽  
Optimum Number ◽  
Bacoside A ◽  
Shoot Production

In the present study the effect of heavy metals (MnSO 4, ZnSO 4 and CuSO 4 ) on adventitious shoot regeneration, biomass and bacoside A accumulation in Bacopa monnieri was carried out. The leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg l -1 kinetin (Kin) with varying concentration of heavy metals (Control: Mn-0.10 mM, Zn-0.03 mM and Cu- 0.0001mM; Mn: 0.20, 0.40, 0.80 and 1.60 mM; Zn: 0.06, 0.12, 0.24 and 0.48 mM; Cu: 0.02, 0.05, 0.10 and 0.20 mM). Optimum number of adventitious shoots (123.50 shoot/explants), fresh weight (3.826 g) and dry weight (0.226 g) of Bacopa monnieri were obtained in the medium with 0.12 mM Zn concentration. The highest production

Methyl jasmonate and salicylic acid synergism enhances bacoside A content in shoot cultures of Bacopa monnieri (L.)

2015 ◽  
Vol 122 (1) ◽  
pp. 9-20 ◽  
Author(s):  
Muthiah Joe Virgin Largia ◽  
Govindan Pothiraj ◽  
Jayabalan Shilpha ◽  
Manikandan Ramesh
Keyword(s):  
Salicylic Acid ◽  
Methyl Jasmonate ◽  
Bacopa Monnieri ◽  
Shoot Cultures ◽  
Bacoside A

scholarly journals Adventitious Shoot Regeneration from In Vitro Leaf Explants of the Peach Rootstock Hansen 536

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 755
Author(s):  
Angela Ricci ◽  
Luca Capriotti ◽  
Bruno Mezzetti ◽  
Oriano Navacchi ◽  
Silvia Sabbadini
Keyword(s):  
In Vitro Regeneration ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Shoot Cultures ◽  
Efficient System ◽  
Regeneration Rate ◽  
Factors Affecting

In the present study, an efficient system for the in vitro regeneration of adventitious shoots from the peach rootstock Hansen 536 leaves has been established. Twenty regeneration media containing McCown Woody Plant Medium (WPM) as a basal salt supplemented with different concentrations and combinations of plant growth regulators (PGRs) were tested. Expanded leaves along with their petiole from 3-week-old elongated in vitro shoot cultures were used as starting explants. The highest regeneration rate (up to 53%) was obtained on WPM basal medium enriched with 15.5 μM N6-benzylaminopurine (BAP). The influences on leaf regeneration of the ethylene inhibitor silver thiosulphate (STS) and of different combinations of antibiotics added to the optimized regeneration medium were also investigated. The use of 10 μM STS or carbenicillin (238 μM) combined with cefotaxime (210 μM) significantly increased the average number of regenerating shoots per leaf compared to the control. In vitro shoots were finally elongated, rooted and successfully acclimatized in the greenhouse. The results achieved in this study advances the knowledge on factors affecting leaf organogenesis in Prunus spp., and the regeneration protocol described looks promising for the optimization of new genetic transformation procedures in Hansen 536 and other peach rootstocks and cultivars.

scholarly journals In Vitro Propagation, Phytochemical and Neuropharmacological Profiles of Bacopa monnieri (L.) Wettst.: A Review

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 411 ◽  
Author(s):  
Partha Sarathi Saha ◽  
Sayantika Sarkar ◽  
Rajendran Jeyasri ◽  
Pandiyan Muthuramalingam ◽  
Manikandan Ramesh ◽  
...  
Keyword(s):  
De Novo ◽  
Therapeutic Potential ◽  
Bacopa Monnieri ◽  
Shoot Biomass ◽  
Multiplication Rate ◽  
Shoot Cultures ◽  
Bioactive Constituents ◽  
Neuropsychiatric Diseases ◽  
Direct Shoot

Bacopa monnieri has been used as a reputed drug in the Indian traditional ayurvedic system for centuries. This medicinal herb with important phytopharmaceuticals has been popularly known as “Brahmi”. In recent years, B. monnieri has been extensively studied for its bioactive constituents, constituents responsible for memory enhancing effect, and also its diverse other useful effects. It possesses many pharmacological activities such as antioxidant, gastrointestinal, endocrine, antimicrobial, anti-inflammatory etc. The plant has been also used for the treatment of neurological and neuropsychiatric diseases. Due to its multipurpose therapeutic potential, micropropagation using axillary meristems and de novo organogenesis has been extensively studied in the species and is being reviewed. High frequency direct shoot organogenesis can be induced in excised leaf and internode explants in the absence of exogenous phytohormones and the rate of induction is enhanced in the presence of exogenous cytokinins, supplements, growth regulators, etc. Using explants from tissue culture raised plants, direct shoot regeneration leading to production of more than 100 rooted plants/explant within 8–12 weeks period with 85%–100% survival in the field after acclimatization can be expected following optimized protocols. Bioreactor based micropropagation was found to increase the multiplication rate of shoot cultures for the commercial propagation of B. monnieri plants. The maximum content of bacosides has been recorded in shoot biomass using an airlift bioreactor system. Further studies for the biosynthesis of bacosides and other secondary metabolites need to be conducted in the species utilizing untransformed shoot cultures in bioreactors.

Statistical optimization for enhanced bacoside A production in plant cell cultures of Bacopa monnieri

2017 ◽  
Vol 133 (2) ◽  
pp. 203-214 ◽  
Author(s):  
J. Leonard ◽  
Bishwanath Seth ◽  
Binod B. Sahu ◽  
V. R. Singh ◽  
Nivedita Patra
Keyword(s):  
Plant Cell ◽  
Cell Cultures ◽  
Bacopa Monnieri ◽  
Statistical Optimization ◽  
Plant Cell Cultures ◽  
Bacoside A

scholarly journals IMPROVED MULTIPLICATION OF MATURE HAZELNUT (CORYLUS AVELLANA L.) IN VITRO

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 693b-693
Author(s):  
Xiaoling Yu ◽  
Barbara M. Reed
Keyword(s):  
Carbon Sources ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Multiplication Rate ◽  
Shoot Cultures ◽  
Mature Trees ◽  
Medium Carbon ◽  
Prolonged Culture ◽  
Corylus Avellana L

Multiplication and elongation of shoot cultures established from mature trees of hazelnut cvs. Nonpareil and Tonda Gentile Romana were affected by changes in basal medium, carbon source and concentration, cytokinin and agar concentration. Explants on DKW medium produced significantly more shoots than those on Anderson medium or modified woody plant medium for chestnut. Explants on DKW medium with 3% glucose or fructose gave more and longer shoots than those with the other carbon sources. Cytokinins 6 benzylaminopurine (BA) and zeatin were more effective in producing shoots than kinetin and 2iP. On BA supplemented medium, the best multiplication rate was obtained with 1.5 - 2.0 mg/l. Explants grown on 0.4% agar produced more shoots than those on 0.6%, however, prolonged culture on 0.4% agar caused vitrification of lower parts of the plants. Shoot multiplication rates of these two cultivars were similar, but `Nonpareil' produced longer shoots than `Tonda Gentile Romana'.

scholarly journals Tissue Culture Regeneration of a Medicinal Plant from Mexico: Piper auritum Kunth.

HortScience ◽  
2006 ◽  
Vol 41 (1) ◽  
pp. 207-209 ◽  
Author(s):  
Fabiola Domínguez ◽  
Xavier Lozoya ◽  
James Simon
Keyword(s):  
Leaf Tissue ◽  
Adventitious Shoot ◽  
Callus Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
Shoot Cultures ◽  
Ms Medium ◽  
Whole Plant ◽  
Half Strength ◽  
Piper Auritum ◽  
Growing Conditions

An efficient whole plant regeneration method from callus cultures of Piper auritum was achieved through organogenesis derived from leaf tissue. Proliferating callus and shoot cultures derived from leaf tissue explants placed on Murashige and Skoog (MS) medium supplemented with 2.0 mg·L–1 2, 4-dichlorophenoxyacetic acid (2,4-D) plus 1.5 mg·L–1 kinetin. Optimum combination of hormones (mg·L–1) for shoot induction was 0.5 2,4-D: 1.5 mg·L–1 kinetin (by volume), that resulted in a high rooting rate (49.6 shoots per explant). All of the plants elongated when using a medium consisting of 0.1 mg·L–1 2,4-D plus 1 mg·L–1 kinetin. Elongated shoots were successfully rooted (100%) on half-strength MS medium supplemented with 2.0 mg·L–1 indole-3-acetic acid. All plantlets survived to the growing conditions of a greenhouse. This study demonstrates that leaf tissue of P. auritum is competent for adventitious shoot regeneration and establishes an efficient and useful protocol for the multiplication and conservation of P. autirum for further investigation of its medicinally active constituents.

scholarly journals Development of Shoot Cultures from Leaf Explant of Portulaca quadrifida L.

2019 ◽  
Vol 11 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Ashutosh PATHAK ◽  
Aruna JOSHI ◽  
Asha SHARMA
Keyword(s):  
Acetic Acid ◽  
Carbon Sources ◽  
Leaf Explants ◽  
Shoot Cultures ◽  
Ms Medium ◽  
Medicinal Value ◽  
In Vitro Shoots ◽  
Shoot Number ◽  
Response Variation

Portulaca quadrifida (Portulacaceae) is an annual succulent herb having medicinal value and is consumed as a vegetable or salads in India. In the present study, leaf explants were inoculated on Murashige and Skoog’s (MS) medium fortified with sucrose (3%) and combinations of N6-benzyladenine (6-BA) and kinetin (KIN) individually and in combination with 1-naphtalene acetic acid (NAA). Rapid regeneration was observed in medium fortified with combinations of 6-BA (8 µM) and NAA (1 µM) which formed 19.40 ± 0.64 shoots with 100% response. Variation in sucrose concentrations (4-6%) was tried but it failed to increase the shoot number. When the optimized medium was fortified with different carbon sources viz. dextrose, glucose and maltose, they could not evoked better response and sucrose proved to be more effective for regeneration. Rooting of in vitro shoots was achieved in ½MS + sucrose (1%) + indole-3-butyric acid (IBA, 2 µM).

Sign in / Sign up

Export Citation Format

Share Document