Effects of macro elements and nitrogen source on biomass accumulation and bacoside A production from adventitious shoot cultures of Bacopa monnieri (L.)

In the present study the effect of heavy metals (MnSO 4, ZnSO 4 and CuSO 4 ) on adventitious shoot regeneration, biomass and bacoside A accumulation in Bacopa monnieri was carried out. The leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg l -1 kinetin (Kin) with varying concentration of heavy metals (Control: Mn-0.10 mM, Zn-0.03 mM and Cu- 0.0001mM; Mn: 0.20, 0.40, 0.80 and 1.60 mM; Zn: 0.06, 0.12, 0.24 and 0.48 mM; Cu: 0.02, 0.05, 0.10 and 0.20 mM). Optimum number of adventitious shoots (123.50 shoot/explants), fresh weight (3.826 g) and dry weight (0.226 g) of Bacopa monnieri were obtained in the medium with 0.12 mM Zn concentration. The highest production

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Methyl jasmonate and salicylic acid synergism enhances bacoside A content in shoot cultures of Bacopa monnieri (L.)

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-015-0745-z ◽

2015 ◽

Vol 122 (1) ◽

pp. 9-20 ◽

Cited By ~ 26

Author(s):

Muthiah Joe Virgin Largia ◽

Govindan Pothiraj ◽

Jayabalan Shilpha ◽

Manikandan Ramesh

Keyword(s):

Salicylic Acid ◽

Methyl Jasmonate ◽

Bacopa Monnieri ◽

Shoot Cultures ◽

Bacoside A

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Adventitious Shoot Regeneration from In Vitro Leaf Explants of the Peach Rootstock Hansen 536

Plants ◽

10.3390/plants9060755 ◽

2020 ◽

Vol 9 (6) ◽

pp. 755

Author(s):

Angela Ricci ◽

Luca Capriotti ◽

Bruno Mezzetti ◽

Oriano Navacchi ◽

Silvia Sabbadini

Keyword(s):

In Vitro Regeneration ◽

Adventitious Shoots ◽

Leaf Explants ◽

Basal Medium ◽

Adventitious Shoot ◽

Shoot Cultures ◽

Efficient System ◽

Regeneration Rate ◽

Factors Affecting

In the present study, an efficient system for the in vitro regeneration of adventitious shoots from the peach rootstock Hansen 536 leaves has been established. Twenty regeneration media containing McCown Woody Plant Medium (WPM) as a basal salt supplemented with different concentrations and combinations of plant growth regulators (PGRs) were tested. Expanded leaves along with their petiole from 3-week-old elongated in vitro shoot cultures were used as starting explants. The highest regeneration rate (up to 53%) was obtained on WPM basal medium enriched with 15.5 μM N6-benzylaminopurine (BAP). The influences on leaf regeneration of the ethylene inhibitor silver thiosulphate (STS) and of different combinations of antibiotics added to the optimized regeneration medium were also investigated. The use of 10 μM STS or carbenicillin (238 μM) combined with cefotaxime (210 μM) significantly increased the average number of regenerating shoots per leaf compared to the control. In vitro shoots were finally elongated, rooted and successfully acclimatized in the greenhouse. The results achieved in this study advances the knowledge on factors affecting leaf organogenesis in Prunus spp., and the regeneration protocol described looks promising for the optimization of new genetic transformation procedures in Hansen 536 and other peach rootstocks and cultivars.

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In Vitro Propagation, Phytochemical and Neuropharmacological Profiles of Bacopa monnieri (L.) Wettst.: A Review

Plants ◽

10.3390/plants9040411 ◽

2020 ◽

Vol 9 (4) ◽

pp. 411 ◽

Cited By ~ 1

Author(s):

Partha Sarathi Saha ◽

Sayantika Sarkar ◽

Rajendran Jeyasri ◽

Pandiyan Muthuramalingam ◽

Manikandan Ramesh ◽

...

Keyword(s):

De Novo ◽

Therapeutic Potential ◽

Bacopa Monnieri ◽

Shoot Biomass ◽

Multiplication Rate ◽

Shoot Cultures ◽

Bioactive Constituents ◽

Neuropsychiatric Diseases ◽

Direct Shoot

Bacopa monnieri has been used as a reputed drug in the Indian traditional ayurvedic system for centuries. This medicinal herb with important phytopharmaceuticals has been popularly known as “Brahmi”. In recent years, B. monnieri has been extensively studied for its bioactive constituents, constituents responsible for memory enhancing effect, and also its diverse other useful effects. It possesses many pharmacological activities such as antioxidant, gastrointestinal, endocrine, antimicrobial, anti-inflammatory etc. The plant has been also used for the treatment of neurological and neuropsychiatric diseases. Due to its multipurpose therapeutic potential, micropropagation using axillary meristems and de novo organogenesis has been extensively studied in the species and is being reviewed. High frequency direct shoot organogenesis can be induced in excised leaf and internode explants in the absence of exogenous phytohormones and the rate of induction is enhanced in the presence of exogenous cytokinins, supplements, growth regulators, etc. Using explants from tissue culture raised plants, direct shoot regeneration leading to production of more than 100 rooted plants/explant within 8–12 weeks period with 85%–100% survival in the field after acclimatization can be expected following optimized protocols. Bioreactor based micropropagation was found to increase the multiplication rate of shoot cultures for the commercial propagation of B. monnieri plants. The maximum content of bacosides has been recorded in shoot biomass using an airlift bioreactor system. Further studies for the biosynthesis of bacosides and other secondary metabolites need to be conducted in the species utilizing untransformed shoot cultures in bioreactors.

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Statistical optimization for enhanced bacoside A production in plant cell cultures of Bacopa monnieri

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-017-1373-6 ◽

2017 ◽

Vol 133 (2) ◽

pp. 203-214 ◽

Cited By ~ 6

Author(s):

J. Leonard ◽

Bishwanath Seth ◽

Binod B. Sahu ◽

V. R. Singh ◽

Nivedita Patra

Keyword(s):

Plant Cell ◽

Cell Cultures ◽

Bacopa Monnieri ◽

Statistical Optimization ◽

Plant Cell Cultures ◽

Bacoside A

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Influence of macro elements and nitrogen source on adventitious root growth and withanolide-A production inWithania somnifera(L.) Dunal

Natural Product Research ◽

10.1080/14786419.2010.490914 ◽

2012 ◽

Vol 26 (5) ◽

pp. 466-473 ◽

Cited By ~ 17

Author(s):

H.N. Murthy ◽

N. Praveen

Keyword(s):

Root Growth ◽

Nitrogen Source ◽

Adventitious Root ◽

Macro Elements ◽

Withanolide A

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Arabidopsis and Lobelia anceps access small peptides as a nitrogen source for growth

Functional Plant Biology ◽

10.1071/fp11077 ◽

2011 ◽

Vol 38 (10) ◽

pp. 788 ◽

Cited By ~ 25

Author(s):

Fiona M. Soper ◽

Chanyarat Paungfoo-Lonhienne ◽

Richard Brackin ◽

Doris Rentsch ◽

Susanne Schmidt ◽

...

Keyword(s):

Amino Acids ◽

Nitrogen Source ◽

Biomass Accumulation ◽

Organic N ◽

Amino Acid Residues ◽

Growth Responses ◽

Small Peptides ◽

Inorganic N ◽

Metabolic Products ◽

Species Specific

While importance of amino acids as a nitrogen source for plants is increasingly recognised, other organic N sources including small peptides have received less attention. We assessed the capacity of functionally different species, annual and nonmycorrhizal Arabidopsis thaliana (L.) Heynh. (Brassicaceae) and perennial Lobelia anceps L.f. (Campanulaceae), to acquire, metabolise and use small peptides as a N source independent of symbionts. Plants were grown axenically on media supplemented with small peptides (2–4 amino acids), amino acids or inorganic N. In A. thaliana, peptides of up to four amino acid residues sustained growth and supported up to 74% of the maximum biomass accumulation achieved with inorganic N. Peptides also supported growth of L. anceps, but to a lesser extent. Using metabolite analysis, a proportion of the peptides supplied in the medium were detected intact in root and shoot tissue together with their metabolic products. Nitrogen source preferences, growth responses and shoot–root biomass allocation were species-specific and suggest caution in the use of Arabidopsis as the sole plant model. In particular, glycine peptides of increasing length induced effects ranging from complete inhibition to marked stimulation of root growth. This study contributes to emerging evidence that plants can acquire and metabolise organic N beyond amino acids.

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Tissue Culture Regeneration of a Medicinal Plant from Mexico: Piper auritum Kunth.

HortScience ◽

10.21273/hortsci.41.1.207 ◽

2006 ◽

Vol 41 (1) ◽

pp. 207-209 ◽

Cited By ~ 1

Author(s):

Fabiola Domínguez ◽

Xavier Lozoya ◽

James Simon

Keyword(s):

Leaf Tissue ◽

Adventitious Shoot ◽

Callus Cultures ◽

Dichlorophenoxyacetic Acid ◽

Shoot Cultures ◽

Ms Medium ◽

Whole Plant ◽

Half Strength ◽

Piper Auritum ◽

Growing Conditions

An efficient whole plant regeneration method from callus cultures of Piper auritum was achieved through organogenesis derived from leaf tissue. Proliferating callus and shoot cultures derived from leaf tissue explants placed on Murashige and Skoog (MS) medium supplemented with 2.0 mg·L–1 2, 4-dichlorophenoxyacetic acid (2,4-D) plus 1.5 mg·L–1 kinetin. Optimum combination of hormones (mg·L–1) for shoot induction was 0.5 2,4-D: 1.5 mg·L–1 kinetin (by volume), that resulted in a high rooting rate (49.6 shoots per explant). All of the plants elongated when using a medium consisting of 0.1 mg·L–1 2,4-D plus 1 mg·L–1 kinetin. Elongated shoots were successfully rooted (100%) on half-strength MS medium supplemented with 2.0 mg·L–1 indole-3-acetic acid. All plantlets survived to the growing conditions of a greenhouse. This study demonstrates that leaf tissue of P. auritum is competent for adventitious shoot regeneration and establishes an efficient and useful protocol for the multiplication and conservation of P. autirum for further investigation of its medicinally active constituents.

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