Production of conjugated linoleic acid by heterologous expression of linoleic acid isomerase in oleaginous fungus Mortierella alpina

2015 ◽  
Vol 37 (10) ◽  
pp. 1983-1992 ◽  
Author(s):  
Danhui Hao ◽  
Haiqin Chen ◽  
Guangfei Hao ◽  
Bo Yang ◽  
Baixi Zhang ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Heterologous Expression ◽  
Conjugated Linoleic Acid ◽  
Mortierella Alpina ◽  
Oleaginous Fungus ◽  
Linoleic Acid Isomerase

Linoleic Acid Isomerase from Propionibacterium acnes: Purification, Characterization, Molecular Cloning, and Heterologous Expression

2007 ◽  
Vol 143 (3) ◽  
pp. 199-211 ◽  
Author(s):  
Ming-De Deng ◽  
Alan D. Grund ◽  
Kenneth J. Schneider ◽  
Kim M. Langley ◽  
Sarah L. Wassink ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Heterologous Expression ◽  
Molecular Cloning ◽  
Propionibacterium Acnes ◽  
Linoleic Acid Isomerase

Molecular Cloning and Heterologous Expression of Linoleic Acid Isomerase Gene from Lactobacillus reuteri and Lactobacillus acidophilus

2012 ◽  
Vol 554-556 ◽  
pp. 1410-1414 ◽  
Author(s):  
Xue Luo ◽  
Lan Wei Zhang ◽  
Tie Nan Luo ◽  
Shu Mei Wang
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Linoleic Acid ◽  
Heterologous Expression ◽  
Lactobacillus Acidophilus ◽  
Lactobacillus Reuteri ◽  
Expression System ◽  
Fusion Partner ◽  
His Tag ◽  
Linoleic Acid Isomerase

The linoleic acid isomerase enzyme from Lactobacillus reuteri and Lactobacillus acidophilus, which were responsible for bioconversion of linoleic acid to cis-9, trans-11 conjugated linoleic acid (c9, t11 CLA) was cloned and overexpressed in Escherichia coli using expression system of pCold-SUMO. The gene was expressed under T7 promotor with a fusion partner of 6×His. Tag at its 5'terminal. After induction by 0.2mM IPTG for 18 h, the recombinant enzyme are expressing in the cytoplasm. The 1776 bp gene from L. reuteri and L. acidophilus encode a linoleic acid isomerase protein with 591 amino acids (64KD).

Conjugated Linoleic Acid Causes a Marked Increase in Liver α-Tocopherol and Liver α-Tocopherol Transfer Protein in C57BL/6 J Mice

2010 ◽  
Vol 80 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Pei-Min Chao ◽  
Wan-Hsuan Chen ◽  
Chun-Huei Liao ◽  
Huey-Mei Shaw
Keyword(s):  
Antioxidant Enzymes ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substances ◽  
Control Groups ◽  
Protein Levels ◽  
Transfer Protein ◽  
And Control

Conjugated linoleic acid (CLA) is a collective term for the positional and geometric isomers of a conjugated diene of linoleic acid (C18:2, n-6). The aims of the present study were to evaluate whether levels of hepatic α-tocopherol, α-tocopherol transfer protein (α-TTP), and antioxidant enzymes in mice were affected by a CLA-supplemented diet. C57BL/6 J mice were divided into the CLA and control groups, which were fed, respectively, a 5 % fat diet with or without 1 g/100 g of CLA (1:1 mixture of cis-9, trans-11 and trans-10, cis-12) for four weeks. α-Tocopherol levels in plasma and liver were significantly higher in the CLA group than in the control group. Liver α-TTP levels were also significantly increased in the CLA group, the α-TTP/β-actin ratio being 2.5-fold higher than that in control mice (p<0.01). Thiobarbituric acid-reactive substances were significantly decreased in the CLA group (p<0.01). There were no significant differences between the two groups in levels of three antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). The accumulation of liver α-tocopherol seen with the CLA diet can be attributed to the antioxidant potential of CLA and the ability of α-TTP induction. The lack of changes in antioxidant enzyme protein levels and the reduced lipid peroxidation in the liver of CLA mice are due to α-tocopherol accumulation.

Sign in / Sign up

Export Citation Format

Share Document