The prognostic significance of STAT3 in invasive breast cancer: analysis of protein and mRNA expressions in large cohorts

(1) Background: Tumour angiogenesis is critical for the progression of neoplasms. A prospective study was designed to examine the utility of stromal cell-derived factor 1α (SDF-1α) and selected vasculo-angiogenic parameters for estimating the probability of disease relapse in 84 primary, operable invasive breast cancer (IBrC) patients (40 (48%) with stage IA and 44 (52%) with stage IIA and IIB). (2) Methods: We explored the prognostic value of the plasma levels of SDF-1α, vascular endothelial growth factor A (VEGF-A), the soluble forms of VEGF receptors type 1 and 2, and the number of circulating endothelial progenitor cells (circulating EPCs) in breast cancer patients. The median follow-up duration was 58 months, with complete follow-up for the first event. (3) Results: According to ROC curve analysis, the optimal cut-off point for SDF-1α (for discriminating between patients at high and low risk of relapse) was 42 pg/mL, providing 57% sensitivity and 75% specificity. Kaplan–Meier curves for disease-free survival (DFS) showed that concentrations of SDF-1α lower than 42 pg/dL together with a VEGFR1 lower than 29.86 pg/mL were significantly associated with shorter DFS in IBrC patients (p = 0.0381). Patients with both SDF-1α lower than 42 pg/dL and a number of circulating EPCs lower than 9.68 cells/µL had significantly shorter DFS (p = 0.0138). (4) Conclusions: Our results imply the clinical usefulness of SDF-1α, sVEGFR1 and the number of circulating EPCs as prognostic markers for breast cancer in clinical settings.

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Prognostic Significance of Molecular Profile in Non-metastatic Invasive Breast Cancer: A Multicentre Study from India

Indian Journal of Gynecologic Oncology ◽

10.1007/s40944-020-0381-3 ◽

2020 ◽

Vol 18 (2) ◽

Author(s):

Krishnakumar Rathnam ◽

K. Niraimathi ◽

Hemant Deepak Shewade ◽

Chenappa N. Patil ◽

Neelesh Reddy ◽

...

Keyword(s):

Breast Cancer ◽

Multicentre Study ◽

Invasive Breast Cancer ◽

Prognostic Significance ◽

Molecular Profile

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Prognostic Significance of a Novel Hypoxia-Regulated Marker, Carbonic Anhydrase IX, in Invasive Breast Carcinoma

Journal of Clinical Oncology ◽

10.1200/jco.2001.19.16.3660 ◽

2001 ◽

Vol 19 (16) ◽

pp. 3660-3668 ◽

Cited By ~ 294

Author(s):

Stephen K. Chia ◽

Charles C. Wykoff ◽

Peter H. Watson ◽

Cheng Han ◽

Russell D. Leek ◽

...

Keyword(s):

Breast Cancer ◽

Overall Survival ◽

Breast Carcinoma ◽

Carbonic Anhydrase ◽

Invasive Breast Cancer ◽

Estrogen Receptor Status ◽

Prognostic Significance ◽

Invasive Breast Carcinoma ◽

Carbonic Anhydrase Ix ◽

Ca Ix

PURPOSE: To assess the frequency of expression and the prognostic significance of a hypoxia-regulated marker, carbonic anhydrase IX (CA IX), in a cohort of patients with invasive breast cancer. PATIENTS AND METHODS: CA IX expression was evaluated by immunohistochemistry with a murine monoclonal antibody, M75, in a series of 103 women treated surgically for invasive breast cancer. The majority of patients were treated with adjuvant hormonal or chemotherapy. The frequency of CA IX expression, its association with recognized prognostic factors, and the relationship with outcome was evaluated by univariate and multivariate statistical analyses. RESULTS: CA IX expression was present in 49 (48%) of 103 cases. The level of CA IX expression was found to be significantly associated with tumor necrosis (P < .001), higher grade (P = .02), and negative estrogen receptor status (P < .001). Furthermore, CA IX expression was associated with a higher relapse rate (P = .004) and a worse overall survival (P = .001). By multivariate analysis, CA IX was also shown to be an independent predictive factor for overall survival (hazard ratio, 2.61; 95% confidence interval, 1.01 to 6.75, P = .05). CONCLUSION: CA IX expression was associated with worse relapse-free survival and overall survival in an unselected cohort of patients with invasive breast carcinoma. The potential role of CA IX as a marker of hypoxia within breast carcinomas was also indicated by a significant association with necrosis. Further work assessing its prognostic significance in breast cancer is warranted, particularly interactions with radiotherapy and chemotherapy resistance.

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P177 The prognostic significance of Ki-67 expression in patients with early invasive breast cancer

The Breast ◽

10.1016/s0960-9776(11)70119-9 ◽

2011 ◽

Vol 20 ◽

pp. S37

Author(s):

T. Utsumi ◽

N. Kobayashi ◽

M. Hikichi ◽

S. Miyajima ◽

K. Ushimado ◽

...

Keyword(s):

Breast Cancer ◽

Invasive Breast Cancer ◽

Prognostic Significance ◽

Ki 67

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The prognostic significance of nuclear DNA content in invasive breast cancer – a study with long-term follow-up

British Journal of Cancer ◽

10.1038/bjc.1989.342 ◽

1989 ◽

Vol 60 (5) ◽

pp. 693-700 ◽

Cited By ~ 89

Author(s):

S Toikkanen ◽

H Joensuu ◽

P Klemi

Keyword(s):

Breast Cancer ◽

Invasive Breast Cancer ◽

Dna Content ◽

Nuclear Dna ◽

Prognostic Significance ◽

Nuclear Dna Content ◽

Long Term Follow Up

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Immunohistochemical Expressions of Senescence-Associated Secretory Phenotype and Its Association With Immune Microenvironments and Clinicopathological Factors in Invasive Breast Cancer

Pathology & Oncology Research ◽

10.3389/pore.2021.1609795 ◽

2021 ◽

Vol 27 ◽

Author(s):

Min Hui Park ◽

Jung Eun Choi ◽

Jae-Ryong Kim ◽

Young Kyung Bae

Keyword(s):

Breast Cancer ◽

Invasive Breast Cancer ◽

Molecular Subtype ◽

Prognostic Significance ◽

Disease Free Survival ◽

Luminal A ◽

Cancer Specific Survival ◽

Ki 67 ◽

Entire Cohort ◽

Secretory Phenotype

This study was undertaken to investigate immunohistochemical expression of the senescence-associated secretory phenotype (SASP) in invasive breast cancer (IBC) tissues and to determine relationships between SASP positivity and tumor microenvironments and the clinicopathological characteristics of IBC. Immunohistochemistry for senescence markers, that is, high mobility group box-1 (HMGB1), p16, p15, and decoy receptor 2 (DCR2), was performed in tissue microarrays of 1140 IBC samples. Cases positive for at least one of these four markers were considered SASP-positive. Relations between SASP and tumor characteristics, including immune microenvironments (stromal tumor-infiltrating lymphocytes [sTILs] density and numbers of intraepithelial CD103-positive [iCD103 + ] lymphocytes) and clinical outcomes were retrospectively evaluated. HMGB1, p16, p15, or DCR2 was positive in 6.7%, 26.6%, 21.1%, and 26.5%, respectively, of the 1,140 cases. Six hundred and five (53.1%) cases were SASP positive, and SASP positivity was significantly associated with histologic grade 3, high-sTIL and iCD103 + lymphocyte counts, absence of ER or PR, and a high Ki-67 index. Although SASP did not predict breast cancer-specific survival (BCSS) or disease-free survival (DFS) in the entire cohort, SASP positivity in luminal A IBC was associated with poor BCSS and DFS. However, patients with SASP-positive TNBC showed better survival than those with SASP-negative TNBC. In multivariate analysis, SASP positivity was an independent prognostic factor in both luminal A IBC and TNBC, although the effect on prognosis was the opposite. In conclusion, SASP would be involved in the modulation of immune microenvironments and tumor progression in IBC, and its prognostic significance depends on molecular subtype.

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Carboxypeptidase N1 is anticipated to be a synergy metrics for chemotherapy effectiveness and prognostic significance in invasive breast cancer

Cancer Cell International ◽

10.1186/s12935-021-02256-5 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Ranliang Cui ◽

Chaomin Wang ◽

Tiantian Li ◽

Jialei Hua ◽

Ting Zhao ◽

...

Keyword(s):

Breast Cancer ◽

Invasive Breast Cancer ◽

Cox Regression ◽

Prognostic Significance ◽

Progression Free Survival ◽

Poor Overall Survival ◽

Tissue Samples ◽

Cox Regression Analysis ◽

Potential Biomarker ◽

Incidence And Mortality

Abstract Background The incidence and mortality of invasive breast cancer (IBC) are increasing annually. Hence, it is urgently needed to determine reliable biomarkers for not only monitoring curative effects, but evaluating prognosis. In present study, we aim to determine the potential role of Carboxypeptidase N1 (CPN1) in IBC tissues on chemotherapeutic efficacy and poor prognosis. Methods The expression level of CPN1 in IBC tissue samples (n = 123) was quantified by tissue microarray technique and immunohistochemical staining. Moreover, sera of IBC patients (n = 34) that underwent three to five consecutive chemotherapy sessions were collected. The patients were randomly stratified into a training (n = 15) as well as a validation group (n = 19). The expression of serum CA153 and CPN1 was quantified by electrochemiluminescence and ELISA assay, respectively. Results By univariate and multivariate Cox regression analysis, we show that CPN1 expression in IBC tissues, as an independent risk factor, is related to a poor overall survival (OS) and progression-free survival (PFS) (P < 0.05). Analysis of the data revealed that CPN1 over-expression could be consistently linked to adverse clinicopathological features such as lymph node metastasis and the pathological stage (pTNM) (P < 0.05). The serum CPN1 level trajectory of individual patients generally decreased during chemotherapy. In line with these findings were changes in the follow-up ultrasonography and a consistent decrease in serum CPN1 levels. The comparison of the area under the receiver operating curves (ROC) revealed that CPN1 has a better surveillance value than CA153 in the training (AUCCPN1 = 0.834 vs. AUCCA153 = 0.724) as well as the validation set (AUCCPN1 = 0.860 vs. AUCCA153 = 0.720) when comparing cycle2 versus cycle3. Conclusions CPN1 is a suitable potential biomarker for chemotherapeutic surveillance purposes as well as being an appropriate prognostic indicator which would support an improved chemotherapy regimen.

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