Permeability Studies on In Vitro Blood–Brain Barrier Models: Physiology, Pathology, and Pharmacology

2005 ◽  
Vol 25 (1) ◽  
pp. 59-127 ◽  
Author(s):  
Mária A. Deli ◽  
Csongor S. Ábrahám ◽  
Yasufumi Kataoka ◽  
Masami Niwa
Keyword(s):  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Blood Brain ◽  
Permeability Studies

scholarly journals Transferrin Functionalized Liposomes Loading Dopamine HCl: Development and Permeability Studies across an In Vitro Model of Human Blood–Brain Barrier

Nanomaterials ◽  
2018 ◽  
Vol 8 (3) ◽  
pp. 178 ◽  
Author(s):  
Antonio Lopalco ◽  
Annalisa Cutrignelli ◽  
Nunzio Denora ◽  
Angela Lopedota ◽  
Massimo Franco ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Human Blood ◽  
In Vitro Model ◽  
Brain Barrier ◽  
Blood Brain ◽  
Vitro Model ◽  
Permeability Studies

scholarly journals A Quasi-Physiological Microfluidic Blood-Brain Barrier Model for Brain Permeability Studies

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1474
Author(s):  
Behnam Noorani ◽  
Aditya Bhalerao ◽  
Snehal Raut ◽  
Ehsan Nozohouri ◽  
Ulrich Bickel ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Time Course ◽  
Induced Pluripotent Stem Cell ◽  
Brain Barrier ◽  
Blood Brain ◽  
Chip Technology ◽  
Blood Brain Barrier Model ◽  
Induced Pluripotent ◽  
Permeability Studies

Microfluidics-based organ-on-a-chip technology allows for developing a new class of in-vitro blood-brain barrier (BBB) models that recapitulate many hemodynamic and architectural features of the brain microvasculature not attainable with conventional two-dimensional platforms. Herein, we describe and validate a novel microfluidic BBB model that closely mimics the one in situ. Induced pluripotent stem cell (iPSC)-derived brain microvascular endothelial cells (BMECs) were juxtaposed with primary human pericytes and astrocytes in a co-culture to enable BBB-specific characteristics, such as low paracellular permeability, efflux activity, and osmotic responses. The permeability coefficients of [13C12] sucrose and [13C6] mannitol were assessed using a highly sensitive LC-MS/MS procedure. The resulting BBB displayed continuous tight-junction patterns, low permeability to mannitol and sucrose, and quasi-physiological responses to hyperosmolar opening and p-glycoprotein inhibitor treatment, as demonstrated by decreased BBB integrity and increased permeability of rhodamine 123, respectively. Astrocytes and pericytes on the abluminal side of the vascular channel provided the environmental cues necessary to form a tight barrier and extend the model’s long-term viability for time-course studies. In conclusion, our novel multi-culture microfluidic platform showcased the ability to replicate a quasi-physiological brain microvascular, thus enabling the development of a highly predictive and translationally relevant BBB model.

scholarly journals Identification of two immortalized cell lines, ECV304 and bEnd3, for in vitro permeability studies of blood-brain barrier

PLoS ONE ◽  
2017 ◽  
Vol 12 (10) ◽  
pp. e0187017 ◽  
Author(s):  
Shu Yang ◽  
Shenghui Mei ◽  
Hong Jin ◽  
Bin Zhu ◽  
Yue Tian ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Cell Lines ◽  
Brain Barrier ◽  
Immortalized Cell Lines ◽  
Blood Brain ◽  
Immortalized Cell ◽  
Permeability Studies
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