Neuron survival in vitro is more influenced by the developmental age of the cells than by glucose condition

In normal mouse embryos developing in vivo, the first appearance of the blastocyst cavity was found to be associated more closely with developmental age, judged by cell number, than with chronological age, i.e. elapsed time since ovulation. When development was slowed by in vitro culture, formation of the blastocoele was delayed. However, cell number itself was not a critical factor, since the number of cells per embryo could be doubled or tripled or halved by experimental manipulation without substantially affecting the timing of blastocoele formation. Experiments in which one cell division was suppressed with cytochalasin-B, leading to tetraploidy, showed that the number of cell divisions since fertilization was also not critical. A possible role is suggested either for nucleocytoplasmic ratio, or for the number of nuclear or chromosomal divisions or DNA replications since fertilization, all of which increase during cleavage.

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In vitro Study of the Effects of Lens Extract on Rat Retinal Neuron Survival and Neurite Outgrowth

Ophthalmic Research ◽

10.1159/000219682 ◽

2009 ◽

Vol 42 (1) ◽

pp. 29-35 ◽

Cited By ~ 8

Author(s):

Yanhua Wang ◽

Yi Wang ◽

Dongwu Wang ◽

Nan Wu ◽

Dongning Liu ◽

...

Keyword(s):

Neurite Outgrowth ◽

In Vitro Study ◽

Vitro Study ◽

Retinal Neuron ◽

Neuron Survival

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miR-204 suppresses cochlear spiral ganglion neuron survival in vitro by targeting TMPRSS3

Hearing Research ◽

10.1016/j.heares.2014.05.002 ◽

2014 ◽

Vol 314 ◽

pp. 60-64 ◽

Cited By ~ 10

Author(s):

Youzhong Li ◽

Anquan Peng ◽

Shenglei Ge ◽

Qin Wang ◽

Jiajia Liu

Keyword(s):

Spiral Ganglion ◽

Ganglion Neuron ◽

Spiral Ganglion Neuron ◽

Neuron Survival ◽

Cochlear Spiral Ganglion

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Activation of mTOR signaling mediates the increased expression of AChE in high glucose condition: in vitro and in vivo evidences

Molecular Neurobiology ◽

10.1007/s12035-015-9425-6 ◽

2015 ◽

Vol 53 (7) ◽

pp. 4972-4980 ◽

Cited By ~ 11

Author(s):

Yao-Wu Liu ◽

Liang Zhang ◽

Yu Li ◽

Ya-Qin Cheng ◽

Xia Zhu ◽

...

Keyword(s):

High Glucose ◽

Mtor Signaling ◽

High Glucose Condition ◽

Glucose Condition

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Circus movements in dissociated cells from two new hybrid frog embryos

Journal of Cell Science ◽

10.1242/jcs.68.1.69 ◽

1984 ◽

Vol 68 (1) ◽

pp. 69-82

Author(s):

K.E. Johnson ◽

M.R. Adelman

Keyword(s):

Cell Surface ◽

Rana Pipiens ◽

Cell Movement ◽

Close Correlation ◽

Cell Movements ◽

Related Cell ◽

Developmental Age ◽

Dissociated Cells

Circus movements are a peculiar kind of cell movement, seen in many amphibian cells, that involve the formation of a hyaline protrusion from the cell surface and rotation of this blister around the circumference of the cell. Studies of cells from normal Rana pipiens embryos have shown a close correlation between circus movements in vitro and gastrulation related cell movements in vivo. Cells from two developmentally arrested hybrid embryos (R. pipiens female X R. sylvatica male and R. pipiens female X R. catesbiana male) are deficient in circus movements to an extent that corresponds to their gastrulation deficiencies. This study reports on the preparation of two new hybrids (R. pipiens female X R. temporaria male (temp) and R. pipiens female X R. clamitans male (clam)). Cells from temp embryos show some circus movements but the proportion of cells displaying such movements does not increase with increasing developmental age to the same extent as is seen in cells from normal embryos. Cells from clam embryos show very few circus movements at any developmental age. These aberrations in the onset and extent of circus movements are discussed in relation to characteristic morphogenetic events that occur in normal embryos and in these two new arrested hybrid embryos.

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SECRETION OF α-AMYLASE BY THE EMBRYONIC CHICK PANCREAS IN VITRO

The Journal of Cell Biology ◽

10.1083/jcb.29.2.287 ◽

1966 ◽

Vol 29 (2) ◽

pp. 287-292 ◽

Cited By ~ 16

Author(s):

R. G. Kulka ◽

U. Yalovsky

Keyword(s):

Digestive Enzymes ◽

Morphological Changes ◽

Chick Embryos ◽

Embryonic Chick ◽

Cholinergic Drugs ◽

Developmental Age

Pancreases taken from chick embryos secrete amylase in vitro when stimulated by cholinergic drugs. Rates of secretion increase with developmental age. The pancreas isolated together with the duodenal loop from the 8 day embryo is already capable of secretion in vitro. It is therefore concluded that the pancreas acquires the ability to secrete digestive enzymes more than 10 days before the beginning of the prominent biochemical and morphological changes associated with the maturation of the gland.

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