Characterization of Bean common mosaic virus and Bean common mosaic necrosis virus isolates in common bean growing areas in Turkey

Bean common mosaic virus (BCMV) exists as a complex of strains classified by reactions to resistance genes found in common bean (Phaseolus vulgaris); seven BCMV pathotypes have been distinguished thus far, numbered I to VII. Virus genetic determinants involved in pathogenicity interactions with resistance genes have not yet been identified. Here, we describe the characterization of two novel field isolates of BCMV that helped to narrow down these genetic determinants interacting with specific P. vulgaris resistance factors. Based on a biological characterization on common bean differentials, both isolates were classified as belonging to pathotype VII, similar to control isolate US10, and both isolates exhibited the B serotype. The whole genome was sequenced for both isolates and found to be 98 to 99% identical to the BCMV isolate RU1 (pathotype VI), and a single name was retained: BCMV RU1-OR. To identify a genetic determinant of BCMV linked to the BCMV pathotype VII, the whole genome was also sequenced for two control isolates, US10 and RU1-P. Inspection of the nucleotide sequences for BCMV RU1-OR and US10 (both pathotype VII) and three closely related sequences of BCMV (RU1-P, RU1-D, and RU1-W, all pathotype VI) revealed that RU1-OR originated through a series of recombination events between US10 and an as-yet-unidentified BCMV parental genome, resulting in changes in virus pathology. The data obtained suggest that a fragment of the RU1-OR genome between positions 723 and 1,961 nucleotides that is common to US10 and RU1-OR in the P1-HC-Pro region of the BCMV genome may be responsible for the ability to overcome resistance in bean conferred by the bc-22 gene. This is the first report of a virus genetic determinant responsible for overcoming a specific BCMV resistance gene in common bean.

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Occurrence and distribution of viruses infecting the bean in Serbia

Archives of Biological Sciences ◽

10.2298/abs1003595p ◽

2010 ◽

Vol 62 (3) ◽

pp. 595-601 ◽

Cited By ~ 4

Author(s):

Dragana Petrovic ◽

Maja Ignjatov ◽

Zorica Nikolic ◽

Milka Vujakovic ◽

Mirjana Vasic ◽

...

Keyword(s):

Mosaic Virus ◽

Viral Infections ◽

Alfalfa Mosaic Virus ◽

Bean Common Mosaic Virus ◽

Yellow Mosaic Virus ◽

Rt Pcr ◽

Necrosis Virus ◽

Pcr Method ◽

Virus Isolates ◽

Viral Isolates

This work describes the incidence and distribution of the most important bean viruses in Serbia: Bean common mosaic virus (BCMV), Bean common mosaic necrosis virus (BCMNV), Bean yellow mosaic virus (BYMV), Cucumber mosaic virus (CMV) and Alfalfa mosaic virus (AMV). The viral isolates were characterized serologically and biologically. BCMV was found in the largest number of plants (30.53%), followed by BCMNV (2.67%), CMV (5.34%), and AMV (3.41%), since BYMV was not determined. Mixed viral infections were found in several samples. The RT-PCR method was used to prove that the tested isolates belong to the BCMV, family Potyviridae and strains Russian and NL-3 D. Results obtained in this work will enable further studies of the genetic variability of bean virus isolates from Serbia. .

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Genetic Characterization of Differential Reactions Among Host Group 3 Common Bean Cultivars to NL-3 K Strain of Bean common mosaic necrosis virus

Phytopathology ◽

10.1094/phyto.2003.93.6.683 ◽

2003 ◽

Vol 93 (6) ◽

pp. 683-690 ◽

Cited By ~ 19

Author(s):

C. A. Strausbaugh ◽

P. N. Miklas ◽

S. P. Singh ◽

J. R. Myers ◽

R. L. Forster

Keyword(s):

Common Bean ◽

Bean Common Mosaic Virus ◽

Breeding Value ◽

Necrosis Virus ◽

Host Group ◽

Differential Cultivars ◽

Allelism Tests ◽

Additional Resistance ◽

Group 3

A previously unrecognized recessive resistance gene (or allele) was identified in three host group (HG) 3 common bean (Phaseolus vulgaris) cvs. Olathe, Victor, and UI 37, based on genetic analysis of plants from five populations screened with the NL-3 K strain of Bean common mosaic necrosis virus (BCMNV). The gene (or allele) was associated with resistance to leaf stunting and deformity and reduction in plant height. The gene (or allele) provides similar, but slightly better resistance than the bc-12 gene that is characteristic of HG 3 cultivars. Traditional HG 3 cultivars like Redlands Greenleaf B with bc-12 are susceptible to NL-3 K, whereas this newly identified gene (or allele) conditions resistance to NL-3 K. Other slight variations in disease reaction pattern to a wide array of bean common mosaic (BCM)-inducing strains were noted among HG 3 differentials, indicating that additional resistance to BCM exists in common bean that remains to be exploited. To gauge the full breeding value of this newly identified gene (or allele), allelism tests with existing genes, namely bc-12, and further characterization of responses to all Bean common mosaic virus (BCMV) and BCMNV strains need to be conducted. Meanwhile, breeders should consider introgressing this more effective gene (or allele) into susceptible cultivars while plant pathologists continue to decipher the genetic variability present among HG 3 differential cultivars.

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Incidence and Characterization of Bean Common Mosaic Virus Isolates in Spanish Bean Fields

Plant Disease ◽

10.1094/pd-79-0079 ◽

1995 ◽

Vol 79 (1) ◽

pp. 79 ◽

Cited By ~ 14

Author(s):

M. Saiz

Keyword(s):

Mosaic Virus ◽

Bean Common Mosaic Virus ◽

Virus Isolates

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Comprehensive Surveys of Bean common mosaic virus and Bean common mosaic necrosis virus and Molecular Evidence for Occurrence of Other Phaseolus vulgaris Viruses in Tanzania

Plant Disease ◽

10.1094/pdis-01-18-0198-re ◽

2018 ◽

Vol 102 (11) ◽

pp. 2361-2370 ◽

Cited By ~ 7

Author(s):

Beatrice Mwaipopo ◽

Susan Nchimbi-Msolla ◽

Paul J. R. Njau ◽

Deogratius Mark ◽

Deusdedith R. Mbanzibwa

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Mosaic Virus ◽

Bean Common Mosaic Virus ◽

Molecular Diagnostic ◽

Diagnostic Tools ◽

Molecular Evidence ◽

Coat Proteins ◽

Necrosis Virus ◽

Virus Diseases

Virus diseases are among the main biotic factors constraining common bean (Phaseolus vulgaris L.) production in Tanzania. Disease management requires information on types, distribution, incidence, and genetic variation of the causal viruses, which is currently limited. Thus, a countrywide comprehensive survey was conducted. Use of a next-generation sequencing technique enabled simultaneous detection of 15 viruses belonging to 11 genera. De novo assembly resulted in many contigs, including complete or nearly complete sequences of Bean common mosaic virus (BCMV), Bean common mosaic necrosis virus (BCMNV), and Southern bean mosaic virus (SBMV). Some viruses (for example, SBMV and Tomato leaf curl Uganda virus-related begomovirus) were detected for the first time in common bean in Tanzania. Visually assessed virus-like disease incidence ranged from 0 to 98% but reverse-transcription polymerase chain reaction-based incidence of BCMV and BCMNV (7,756 samples) was mostly less than 40%. The Sanger-based nucleotide sequences encoding coat proteins of BCMV and BCMNV isolates were 90.2 to 100% and 97.1 to 100% identical to each other, respectively. Phylogenetic analysis showed that BCMV isolates were more diverse than BCMNV isolates. The information generated in this study will contribute to the development of molecular diagnostic tools and strategies for management of virus diseases nationally and internationally. [Formula: see text] Copyright © 2018 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .

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