Detection of Xanthomonas axonopodis pv. manihotis, the causal agent of cassava bacterial blight diseases in cassava (Manihot esculenta) in Ghana by polymerase chain reaction

2017 ◽  
Vol 150 (2) ◽  
pp. 471-484
Author(s):  
Muntala Abdulai ◽  
Hüseyin Basım ◽  
Esin Basım ◽  
Derya Baki ◽  
Nurhan Öztürk
Keyword(s):  
Polymerase Chain Reaction ◽  
Manihot Esculenta ◽  
Bacterial Blight ◽  
Causal Agent ◽  
Chain Reaction ◽  
Cassava Bacterial Blight ◽  
Xanthomonas Axonopodis ◽  
Polymerase Chain

scholarly journals Detection of the Cassava Bacterial Blight Pathogen, Xanthomonas axonopodis pv. manihotis, by Polymerase Chain Reaction

Plant Disease ◽  
1998 ◽  
Vol 82 (1) ◽  
pp. 79-83 ◽  
Author(s):  
V. Verdier ◽  
G. Mosquera ◽  
K. Assigbétsé
Keyword(s):  
Polymerase Chain Reaction ◽  
Bacterial Blight ◽  
Pathogen Detection ◽  
Chain Reaction ◽  
Direct Pcr ◽  
Cassava Bacterial Blight ◽  
Xanthomonas Axonopodis ◽  
Planting Material ◽  
Polymerase Chain ◽  
Pcr Assays

Cassava bacterial blight, caused by Xanthomonas axonopodis pv. manihotis, is of significant concern wherever cassava is grown. The movement of infected, asymptomatic stems is a major means of pathogen dispersal. A reliable and sensitive diagnostic procedure is necessary for the safe movement of cassava planting material. We used a cloned and sequenced pathogenicity gene of X. axonopodis pv. manihotis to develop a polymerase chain reaction (PCR) test for this pathogen. A set of primers directed the amplification of an 898-bp fragment in all 107 pathogenic strains of X. axonopodis pv. manihotis tested. PCR products were not observed when genomic DNA was tested for 27 strains of other xanthomonads, for saprophytic bacteria, or for five nonpathogenic strains of X. axonopodis pv. manihotis. The primers worked well for pathogen detection in direct PCR assays of X. axonopodis pv. manihotis colonies grown on liquid medium and in PCR assays of extracts from leaf and stem lesions. The minimum number of cells that could be detected from cassava stem and leaf lesions was 3 × 102 to 104 CFU/ml. The PCR assays proved to be relativyel sensitive and could become very useful in detecting the pathogen in cassava planting material.

AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)

Genome ◽  
1999 ◽  
Vol 42 (2) ◽  
pp. 163-172 ◽  
Author(s):  
Gilda Sanchez ◽  
Silvia Restrepo ◽  
Myriam-Cristina Duque ◽  
Martin Fregene ◽  
Merideth Bonierbale ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Manihot Esculenta ◽  
Bacterial Blight ◽  
Multiple Correspondence Analysis ◽  
Aflp Analysis ◽  
Resistance Screening ◽  
Base Resistance ◽  
Cassava Bacterial Blight ◽  
Xanthomonas Axonopodis ◽  
Cassava Varieties

Cassava bacterial blight (CBB) is caused by Xanthomonas axonopodis pv. manihotis (Xam). Resistance is found in Manihot esculenta and, in addition, has been introgressed from a wild relative, M. glaziovii. The resistance is thought to be polygenic and additively inherited. Ninety-three varieties of M. esculenta (Crantz) were assessed by AFLPs for genetic diversity and for resistance to CBB. AFLP analysis was performed using two primer combinations and a 79.2% level of polymorphism was found. The phenogram obtained showed between 74% and 96% genetic similarity among all cassava accessions analysed. The analysis permitted the unique identification of each individual. Two Xam strains were used for resistance screening. Variation in the reaction of cassava varieties to Xam strains was observed for all plant accessions. The correlation of resistance to both strains, had a coefficient of 0.53, suggesting the independence of resistance to each strain. Multiple correspondence analysis showed a random distribution of the resistance/susceptibility response with respect to overall genetic diversity as measured by AFLP analysis. A total heterozygosity index was calculated to determine the diversity within clusters as well as among them. Our results demonstrate that resistance to CBB is broadly distributed in cassava germplasm and that AFLP analysis is an effective and efficient means of providing quantitative estimates of genetic similarities among cassava accessions.Key words: amplified fragment length polymorphism, genetic base, resistance screening, Xanthomonas axonopodis pv. manihotis.

scholarly journals Molecular Identification of Bacterial Pathogen Infecting Coconut Leaf Beetle

Buletin Palma ◽  
2016 ◽  
Vol 16 (2) ◽  
pp. 147
Author(s):  
JELFINA C. ALOUW ◽  
DIANA NOVIANTI ◽  
MELDY L.A. HOSANG
Keyword(s):  
Polymerase Chain Reaction ◽  
16S Rrna ◽  
Serratia Marcescens ◽  
Molecular Identification ◽  
Ribosomal Rna ◽  
Causal Agent ◽  
16S Ribosomal Rna ◽  
Font Size ◽  
Chain Reaction ◽  
Polymerase Chain

<p><span style="font-size: medium;">ABSTRACT </span></p><p>Many species of microorganisms can cause diseases and mortality of insect pests. Accurate detection and identification of the entomophatogens are essential for development of biological control agent to the pest. Brontispa longissima, a serious and invasive pest of coconut, was infected by bacterium causing mortality of the larvae and pupae in coconut field. Objective of the research was to identify bacterium as a causal agent of the field-infected B. longissima using molecular  technique.  Research  was  conducted  between  April  and  August 2011.  Molecular  identification  using polymerase chain reaction (PCR) amplification of 16s ribosomal RNA of the infected larvae and sequencing of the gene showed that Serratia marcescens is the causal agent of the disease.</p><p>Keywords: Brontispa longissima, coconut, 16s rRNA, Serratia marcescens.</p><p> </p><p><span style="font-size: medium;">Identifikasi Molekular Bakteri Pathogen yang Menginfeksi Hama Daun Kelapa <br />Brontispa longissima(Coleoptera:Chrysomelidae)</span></p><p><span style="font-size: medium;">ABSTRAK </span></p><p>Banyak mikroorganisme dapat menimbulkan penyakit pada serangga hama.  Deteksi dan identifikasi yang akurat dari  pathogen  penyebab  penyakit  pada  serangga (entomopathogen)  hama  merupakan  tahap  yang  penting  dalam  pengembangan pengendalian biologi untuk hama tersebut.  Brontispa longissima sebagai hama penting dan bersifat  invasif pada tanaman kelapa diinfeksi oleh sejenis bakteri yang menyebabkan kematian larva dan pupa dari serangga  tersebut di lapangan. Penelitian ini bertujuan untuk mengidentifikasi organisme penyebab penyakit pada hama B. longissima dengan menggunakan teknik molekuler. Penelitian dilaksanakan pada bulan April sampai dengan Agustus  2011. Identifikasi bakteri dilakukan dengan mengamplifikasi 16s ribosomal RNA dari larva yang terinfeksi dengan menggunakan PCR (polymerase chain reaction).  Hasil analisis sekuens nukleotida 16s ribosomal RNA dari larva yang terinfeksi menunjukkan bahwa Serratia marcescens adalah bakteri penyebab dari penyakit tersebut.</p><p>Kata kunci: Brontispa longissima, kelapa, 16s rRNA, Serratia marcescens.</p>

scholarly journals Low incidence of peach latent mosaic viroid in peach mother blocks in Serbia

2014 ◽  
Vol 29 (2) ◽  
pp. 109-114
Author(s):  
Darko Jevremovic ◽  
Svetlana Paunovic
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Mosaic Disease ◽  
Causal Agent ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Low Incidence

Peach latent mosaic viroid (PLMVd) is the causal agent of peach latent mosaic disease that is common on peaches and nectarines worldwide. Most of the isolates do not cause any symptoms on the foliage and the disease may be latent for years. A survey to investigate the presence of PLMVd in selected peach mother blocks in 9 Serbian districts was carried out in 2011 through 2013. A total of 315 trees/samples originating from 43 mother blocks, representing 35 peach and nectarine varieties and 2 rootstocks, were tested by Reverse Transcription - Polymerase Chain Reaction (RT-PCR). PLMVd was detected in 13 samples (4.13%) belonging to 7 varieties and one vineyard peach rootstock. Infected samples were found in 7 mother blocks from 3 districts. Our results indicated a low incidence of PLMVd in the analyzed peach mother blocks.

Cloning of MeGolS5 Promoter from Cassava (Manihot esculenta Crantz) and Expression Analysis in Abiotic Stress of MeGolS5

2014 ◽  
Vol 707 ◽  
pp. 126-132
Author(s):  
Yu Qing Wang ◽  
Jie Fan ◽  
Rui Mei Li ◽  
Fan Zhang ◽  
Meng Ting Geng ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Abscisic Acid ◽  
Abiotic Stresses ◽  
Manihot Esculenta ◽  
Genomic Dna ◽  
Promoter Sequence ◽  
Tata Box ◽  
Inverse Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain

The MeGolS5 promoter fragment (1492 bp) was amplified from the genomic DNA of Manihot esculenta Crantz by inverse polymerase chain reaction. Promoter sequence analysis by PLACE and PlantCARE showed that the cloned fragment contained several putative cis-elements, such as abscisic acid response element (ABRE), heat shock elements (HSE), as well as TATA-Box and CAAT-Box. The expression prfile of MeGolS5 shows that the gene is induced by several abiotic stresses, such as salt, drought, H2O2 and ABA.

scholarly journals Light Microscopy and PCR Based Detection of the Causal Agent of Leaf Mosaic of Jute

2013 ◽  
Vol 22 (1-2) ◽  
pp. 19-25
Author(s):  
KMG Dastogeer ◽  
MA Ali ◽  
M Ashrafuzzaman
Keyword(s):  
Polymerase Chain Reaction ◽  
Light Microscope ◽  
Inclusion Bodies ◽  
Causal Agent ◽  
Healthy Plant ◽  
Infected Leaf ◽  
Chain Reaction ◽  
Degenerate Primers ◽  
Leaf Tissues ◽  
Polymerase Chain

Leaf mosaic transmitted by whitefly is a devastating disease of jute. It is thought to be caused by a virus belonging to begomovirus genus under geminivirus family. To confirm the identity of the causal agent, infected and healthy leaves were studied using light microscope and by using polymerase chain reaction (PCR) technique of DNA. The inclusion bodies were observed under light microscope as large, blue-violet, prominent inclusion bodies in the nucleus of the infected leaf tissues. In molecular detection technique DNA from infected and healthy plants was extracted and analyzed by polymerase chain reaction (PCR) using degenerate primers PALIv1978/PARIc496. PCR fragment of the expected size 1.2kb for the common region (CR) in the geminivirus were obtained from infected plants. DNA collected from healthy plant did not show any band during electrophoresis. Therefore, it can be concluded that leaf mosaic of jute is cause by a virus.DOI: http://dx.doi.org/10.3329/pa.v22i1-2.16463 Progress. Agric. 22(1 & 2): 19 - 25, 2011

Sign in / Sign up

Export Citation Format

Share Document