Insight into inducing disease resistance with Allorhizobium vitis strain ARK-1, a biological control agent against grapevine crown gall disease

2022 ◽  
Author(s):  
Akira Kawaguchi ◽  
Yoshiteru Noutoshi
Keyword(s):  
Biological Control ◽  
Disease Resistance ◽  
Crown Gall ◽  
Biological Control Agent ◽  
Control Agent ◽  
Crown Gall Disease ◽  
Allorhizobium Vitis ◽  
Grapevine Crown Gall ◽  
Insight Into

scholarly journals Complete Genome Sequence Data of the Grapevine Crown Gall inhibiting bacteria Allorhizobium vitis Strain F2/5.

2021 ◽  
Author(s):  
Hangwei Xi ◽  
Joshua Grist ◽  
Maarten Ryder ◽  
Iain Searle
Keyword(s):  
Genome Sequence ◽  
Crown Gall ◽  
Sequence Data ◽  
Biological Control Agent ◽  
Control Agent ◽  
Protein Coding ◽  
Crown Gall Disease ◽  
Allorhizobium Vitis ◽  
Grapevine Crown Gall ◽  
Circular Chromosomes

Crown gall disease in grapevine is caused by pathogenic strains of Allorhizobium vitis. A. vitis strain F2/5 is a non-pathogenic biocontrol agent that was previously shown to act as a biological control agent to crown gall disease and first isolated from South Africa. Here, we present the complete assembled genome and is 5.94 Mb in length with 5,414 predicted protein-coding sequences, has two circular chromosomes and five plasmids. The genome sequence has no detectable T-DNA border sequences and is missing key virulence genes which is consistent with the bacteria being non-pathogenic. The F2/5 genome sequence could contribute to understanding the molecular basis underlying the biocontrol activity.

scholarly journals Characterization of Migration and Induced Resistance of Rhizobium Vitis Strain ARK-1, a Biological Control Agent Against Grapevine Crown Gall Disease

2020 ◽  
Author(s):  
Akira Kawaguchi ◽  
Yoshiteru Noutoshi
Keyword(s):  
Crown Gall ◽  
Temporal Dynamics ◽  
Biological Control Agent ◽  
Control Agent ◽  
Systemic Resistance ◽  
Marker Genes ◽  
Cell Detection ◽  
Induced Expression ◽  
Crown Gall Disease ◽  
Grapevine Crown Gall

Abstract A nonpathogenic strain ARK-1 of Rhizobium vitis has an antagonistic activity toward tumorigenic (Ti) strain of R. vitis, a causal agent of grapevine crown gall disease, and works as a biocontrol agent. We have demonstrated that the gall formation was fully suppressed when ARK-1 was co-inoculated with Ti into the grapevine stem at a 1:1 ratio. For practical use of ARK-1 in agriculture, understanding the temporal dynamics of the bacterial habitat on host plants and the biocontrol property are needed in order to develop proper application methods. Here we demonstrated that the gall incidence by Ti was reduced to about 50% when ARK-1 was pre-inoculated at both upper and lower positions on the grapevine stem 3 cm away from the Ti-inoculation point 5 days before. The bacterial cell detection assay in the grapevine tissue revealed that ARK-1 could migrate at least 3 cm in 5 days. Inoculations of ARK-1 or Ti induced expression of marker genes for defense-related phytohormones such as salicylic acid, jasmonic acid, and ethylene in grapevine within 3 days but they were diminished by 6 days. Inoculation of Ti 5 days after ARK-1 pre-inoculation induced expression of the marker genes except for the LOX-9 gene in a basically similar way to those without the pre-inoculation, suggesting that ARK-1 did not induce typical acquired systemic resistance or induced systemic resistance in grapevine, while the transcript of LOX-9 was detected at 24 and 48 hours after the Ti inoculation when ARK-1 was pre-inoculated, unlike the un-inoculated condition. ARK-1 primed the induction of certain defense genes and it may take part in its biocontrol activity.

scholarly journals Complete Genome Sequence Data of Nonpathogenic Strain Rhizobium vitis VAR03-1, a Biological Control Agent for Grapevine Crown Gall Disease

2020 ◽  
Vol 33 (12) ◽  
pp. 1451-1453
Author(s):  
Yoshiteru Noutoshi ◽  
Atsushi Toyoda ◽  
Tomoya Ishii ◽  
Kirara Saito ◽  
Megumi Watanabe ◽  
...  
Keyword(s):  
Biological Control ◽  
Genome Sequence ◽  
Crown Gall ◽  
Complete Genome ◽  
Sequence Data ◽  
Biological Control Agent ◽  
Suppressive Effect ◽  
Control Agent ◽  
Crown Gall Disease ◽  
Nonpathogenic Strain

Crown gall disease in grapevine is caused by pathogenic strains of Rhizobium vitis with a tumor-inducing (Ti) plasmids. A nonpathogenic strain, VAR03-1 of R. vitis, has been isolated from the grapevine root of nursery stock and it was shown to act as a biological control agent to crown gall disease. Its disease-suppressive effect was observed even when it was coinoculated with the pathogen in a 1:1 ratio. Here, we present the complete genome data of R. vitis VAR03-1, assembled by sequencing reads obtained by both PacBio and Illumina technologies with annotation. This genome sequence could contribute to investigations of the molecular basis underlying the biocontrol activity as well as the root-colonization ability of this bacterial strain. [Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

scholarly journals Antagonistic potential of native agrocin-producing non-pathogenic Agrobacterium tumefaciens strain UHFBA-218 to control crown gall in peach

2017 ◽  
Vol 97 (1) ◽  
pp. 1-11
Author(s):  
Aditi Sharma ◽  
A. K. Gupta ◽  
K. Khosla ◽  
Rishi Mahajan ◽  
Bharti ◽  
...  
Keyword(s):  
Biological Control ◽  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Room Temperature ◽  
Biological Control Agent ◽  
Control Agent ◽  
Stone Fruits ◽  
Copper Oxychloride ◽  
Antagonistic Potential ◽  
Different Doses

A non-pathogenic agrocin-producing native isolate ofAgrobacterium tumefaciensstrain UHFBA-218 was tested as a biological control agent against the peach crown gall. This strain was compatible with all the recommended pesticides used in stone fruits in the integrated pest management (IPM) module, except for copper oxychloride, which was detrimental to its growth. Upon artificial co-inoculation of 4-wk-old plants of tomato var. Solan Gola withA. tumefaciensstrain UHFBA-218 and tumorigenicA. tumefaciensstrain Peach 2E-10, out of the 27 isolates recovered, six were transconjugants showing selective acquisition of tumorigenic factors as made evident by amplification withiptandvirD2primers, whereas the rest of the isolates did not acquire any of these tumorigenic factors. A white stone powder-based formulation of this isolate (103.3 × 108cfu g-1) retained appreciable viability for up to 6 months at room temperature. When peach roots and seeds were soaked in cell suspensions of different doses of a white stone powder-based bioformulation of UHFBA-218 before planting in the field, the number of plants with tumours was reduced, with the lowest incidence of crown gall being observed in the 0.1% UHFBA-218 root dip treatment, i.e. 1.48% and 0.80% during the years 2013 and 2014, respectively. No incidence of crown gall was observed in the three seed dip treatments, i.e. 30-min dip in UHFBA-218 followed by 1 h of shade drying, stratified seeds dipped for 30 min in 0.1% suspensions of strains UHFBA-218 or K84 followed by 1 h of shade drying before sowing, as compared with 14.76% incidence in untreated plants.

scholarly journals Origin of Pathogens of Grapevine Crown Gall Disease in Hokkaido in Japan as Characterized by Molecular Epidemiology of Allorhizobium vitis Strains

Life ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1265
Author(s):  
Akira Kawaguchi ◽  
Teruo Sone ◽  
Sunao Ochi ◽  
Yosuke Matsushita ◽  
Yoshiteru Noutoshi ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Molecular Typing ◽  
Crown Gall ◽  
Crown Gall Disease ◽  
Group A ◽  
Infection Source ◽  
Allorhizobium Vitis ◽  
Grapevine Crown Gall ◽  
Globally Distributed ◽  
Important Disease

Crown gall is a globally distributed and economically important disease of grapevine and other important crop plants. The causal agent of grapevine crown gall is tumorigenic Allorhizobium vitis (Ti) strains that harbor a tumor-inducing plasmid (pTi). The epidemic of grapevine crown gall has not been widely elucidated. In this study, we investigated the genetic diversity of 89 strains of Ti and nonpathogenic A. vitis to clarify their molecular epidemiology. Multi-locus sequence analysis (MLSA) of the partial nucleotide sequences of pyrG, recA, and rpoD was performed for molecular typing of A. vitis strains isolated from grapevines with crown gall symptoms grown in 30 different vineyards, five different countries, mainly in Japan, and seven genomic groups A to F were obtained. The results of MLSA and logistic regression indicated that the population of genetic group A was significantly related to a range of prefectures and that the epidemic of group A strains originated mainly in Hokkaido in Japan through soil infection. Moreover, group E strains could have been transported by infected nursery stocks. In conclusion, this study indicates that both soil infection and transporting of infected nursery stocks are working as infection source in Hokkaido.

scholarly journals Biological Control of Crown Gall of Grapevine, Rose, and Tomato by Nonpathogenic Agrobacterium vitis Strain VAR03-1

2008 ◽  
Vol 98 (11) ◽  
pp. 1218-1225 ◽  
Author(s):  
A. Kawaguchi ◽  
K. Inoue ◽  
Y. Ichinose
Keyword(s):  
Biological Control ◽  
Crown Gall ◽  
Biological Control Agent ◽  
Inhibitory Effect ◽  
Control Agent ◽  
Inhibitory Effects ◽  
Agrobacterium Vitis ◽  
Colony Forming Units ◽  
Nonpathogenic Strain ◽  
A Cell

A nonpathogenic strain of Agrobacterium vitis VAR03-1 was tested as a biological control agent for crown gall of grapevine (Vitis vinifera). When roots of grapevine, rose (Rose multiflora), and tomato (Lycopersicon esculentum) were soaked in a cell suspension of antagonists before planting in soil infested with tumorigenic A. vitis, A. rhizogenes, and A. tumefaciens, respectively, treatment with VAR03-1 significantly reduced the number of plants with tumors and disease severity in the three plant species. The inhibitory effects of treatment with VAR03-1 and the nonpathogenic A. rhizogenes strain K84 on crown gall of rose and tomato were almost identical, and the inhibitory effect of VAR03-1 on grapevine was superior to that of K84. Moreover, VAR03-1 greatly controlled crown gall of grapevine due to tumorigenic A. vitis in the field. VAR03-1 established populations averaging 106 colony forming units (CFU)/g of root in the rhizosphere of grapevine and persisted on roots for 2 years. VAR03-1 was bacteriocinogenic, producing a halo of inhibition against those three species of Agrobacterium. This is the first report that a nonpathogenic strain, VAR03-1, can effectively control crown gall caused by tumorigenic A. vitis, A. rhizogenes, and A. tumefaciens.

scholarly journals Construction of a Range of Derivatives of the Biological Control Strain Agrobacterium rhizogenes K84: a Study of Factors Involved in Biological Control of Crown Gall Disease

1998 ◽  
Vol 64 (10) ◽  
pp. 3977-3982 ◽  
Author(s):  
Nicholas C. McClure ◽  
Ali-Reza Ahmadi ◽  
Bruce G. Clare
Keyword(s):  
Biological Control ◽  
Agrobacterium Rhizogenes ◽  
Crown Gall ◽  
Biological Control Agent ◽  
Control Process ◽  
Dry Weight ◽  
Control Strain ◽  
Crown Gall Disease ◽  
Derivatives Of

ABSTRACT The biological control strain Agrobacterium rhizogenesK84 is an effective agent in the control of Agrobacteriumpathogens, the causative agents of crown gall disease. A number of factors are thought to play a role in the control process, including production of the specific agrocins 84 and 434, which differ in the spectra of pathogenic strains that they inhibit in vitro. A range of derivatives of strain K84 has been developed with every combination of the three resident plasmids, pAgK84, pAgK434, and pAtK84b, including a plasmid-free strain. These derivatives produced either both, one, or neither of the characterized agrocins 84 and 434 and were isolated by plasmid curing, conjugation, and Tn5 transposon mutagenesis. The ability of the derivative strains to inhibit gall formation on almond roots was compared to that of the wild-type K84 parent. Treatment with the plasmid-free derivative did not result in a significant level of control of an A. rhizogenes pathogen based on numbers or dry weight of galls formed on injured almond roots. The presence of plasmid pAgK84, pAgK434, or pAtK84b significantly enhanced the biological control efficacy of K84 derivatives, and the highest level of control was observed with strains harboring two or more plasmids. The results observed with strains deficient in agrocin 434 production suggest that this product may play an important role in the biological control of A. rhizogenes pathogens. The involvement of plasmid pAgK84b in biological control has not previously been reported. This study supports the conclusion that multiple factors are involved in the success of strain K84 as a biological control agent.

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