The fission yeast bromodomain protein Bdf2 is required for growth of cells with circular chromosomes

Circular chromosomes have frequently been observed in tumors of mesenchymal origin. In the fission yeast Schizosaccharomyces pombe, deletion of pot1+ results in rapid telomere loss, and the resulting survivors have circular chromosomes. Fission yeast has two bromodomains and extra-terminal (BET) proteins, Bdf1 and Bdf2; both are required for maintaining acetylated histones. Here, we found that bdf2, but not bdf1, was synthetically lethal with pot1. We also obtained a temperature-sensitive bdf2-ts mutant, which can grow at high temperatures but becomes camptothecin sensitive. This suggests that Bdf2 is defective at high temperatures. The cell cycle of the pot1 bdf2-ts mutant was delayed in the G2 and/or M phase at a semi-permissive temperature. Furthermore, a temperature-sensitive mutant of mst1, which encodes histone acetyltransferase, showed a synthetic growth defect with a pot1 disruptant at a semi-permissive temperature. Our results suggest that Bdf2 and Mst1 are required for the growth of cells with circular chromosomes.

Complete Genome Sequences of Two Nosocomiicoccus ampullae Strains and a Growth-Adapted Mutant

Here, we present the circular and complete genome sequences of the Nosocomiicoccus ampullae isolate 19-00310 and type strain DSM 19163. To our knowledge, these represent the first complete, circular chromosomes in the entire genus. Sequencing of a growth-adapted mutant suggests iron availability as a factor for growth improvement.

Complete Genome Sequence of Leptospira kobayashii Strain E30, Isolated from Soil in Japan

The spirochete bacterium Leptospira kobayashii is a recently designated species of the genus Leptospira . Here, we report the complete genome sequence of L. kobayashii strain E30, consisting of two circular chromosomes and two plasmids.

Complete Genome Sequence Data of the Grapevine Crown Gall inhibiting bacteria Allorhizobium vitis Strain F2/5.

Crown gall disease in grapevine is caused by pathogenic strains of Allorhizobium vitis. A. vitis strain F2/5 is a non-pathogenic biocontrol agent that was previously shown to act as a biological control agent to crown gall disease and first isolated from South Africa. Here, we present the complete assembled genome and is 5.94 Mb in length with 5,414 predicted protein-coding sequences, has two circular chromosomes and five plasmids. The genome sequence has no detectable T-DNA border sequences and is missing key virulence genes which is consistent with the bacteria being non-pathogenic. The F2/5 genome sequence could contribute to understanding the molecular basis underlying the biocontrol activity.

Complete Genome Sequences of Pseudomonas lundensis Strains M101 and M105, Isolated from 1% Pasteurized Milk

Here, we report the complete genome sequences of two strains of Pseudomonas lundensis , M101 and M105, which were isolated from 1% pasteurized milk. Long-read sequencing was performed using a MinION sequencer, and reads were assembled into circular chromosomes of 4,842,187 bp and 4,814,486 bp for M101 and M105, respectively. Both strains had additional plasmid sequences.

Complete Genome Sequences of Three Human Oral Treponema parvum Isolates

Treponema parvum is a spirochete bacterium associated with human and animal oral/nonoral soft tissue infections. Here, we report the complete genome sequences of three human oral isolates of T. parvum , namely, ATCC 700770 T (OMZ 833 T ), ATCC 700773 (OMZ 842), and OMZ 843, which possess circular chromosomes of a median size of 2.63 Mb.

Complete Genome Sequences of Two Corynebacterium macginleyi Strains Isolated from Infectious Keratitis

ABSTRACT Corynebacterium macginleyi is a slow-growing, lipid-requiring bacterium that may cause ocular infections. Here, we report the complete genome sequences of two strains, T160811 and T180208, isolated from infectious keratitis. The two genomes consist of circular chromosomes of 2,431,961 bp and 2,481,998 bp, respectively, and contain high numbers of repetitive elements.

Complete Genome Sequence Resource for the Endophytic Burkholderia sp. Strain MS389 Isolated from a Healthy Soybean Growing Adjacent to Charcoal Rot Disease Patch

Burkholderia sp. strain MS389, an endophytic bacterium, was isolated from a healthy soybean plant growing adjacent to a patch of plants affected by charcoal rot disease, caused by the fungal pathogen Macrophomina phaseolina. Preliminary studies demonstrated that strain MS389 possesses antimicrobial activities against multiple plant pathogens. Burkholderia sp. strain MS389 was found to have three circular chromosomes of 3,563,380 bp, 3,002,449bp, and 1,180,421 bp in size, respectively. The 7,746,250-bp genome, with 66.73% G+C content, harbors 6,756 protein coding genes in the predicted 6,985 genes. In total, 18 rRNAs, 68 tRNAs, 4 ncRNAs were identified and 139 pseudogenes were annotated as well. The findings of this study will provide valuable data to explore the antimicrobial mechanisms of the endophytic bacterial strain.

Complete Genome Sequence of Pseudoalteromonas sp. Strain LC2018020214, a Bacterium Isolated from Natural Seawater

ABSTRACT Pseudoalteromonas is a genus widely distributed in the ocean and displays antibacterial and antifouling activities. We isolated a Pseudoalteromonas sp. strain (LC2018020214) from coastal water of Qingdao, China, and assembled its complete genome. The genome consists of two circular chromosomes with lengths of 3,700,777 bp and 817,517 bp, respectively, and 3,866 coding sequences.

Complete Genome Sequences of Staphylococcus argenteus Tokyo13064 and Tokyo13069, Isolated from Specimens Obtained during a Food Poisoning Outbreak in Tokyo, Japan

ABSTRACT The complete genome sequences of two Staphylococcus argenteus strains, Tokyo13064 and Tokyo13069, isolated from human feces and suspected causative foods during a staphylococcal food poisoning outbreak, consist of 2,750,811-bp and 2,751,556-bp circular chromosomes and 2,543 and 2,548 genome annotation-predicted coding DNA sequences, respectively, with 19 rRNAs, 61 tRNAs, and 1 CRISPR each.