Efficiency of different PCR-based marker systems in assessing genetic diversity among winter rye (Secale cereale L.) inbred lines

Euphytica ◽  
2005 ◽  
Vol 146 (1-2) ◽  
pp. 109-116 ◽  
Author(s):  
Hanna Bolibok ◽  
Monika Rakoczy-Trojanowska ◽  
Aneta Hromada ◽  
Robert Pietrzykowski
Keyword(s):  
Genetic Diversity ◽  
Secale Cereale ◽  
Inbred Lines ◽  
Winter Rye ◽  
Secale Cereale L

THE TRANSFER OF A CYTOPLASMIC MALE-STERILITY SYSTEM TO SPRING RYE

1979 ◽  
Vol 59 (1) ◽  
pp. 163-169 ◽  
Author(s):  
G. J. SCOLES ◽  
L. E. EVANS
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Environmental Effects ◽  
Secale Cereale ◽  
Fertility Restoration ◽  
Inbred Lines ◽  
Winter Rye ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Secale Cereale L ◽  
Restoration Of Fertility

Six inbred lines of spring rye (Secale cereale L.) and an open-pollinated cultivar of spring rye were crossed with a cytoplasmic male-sterile line of winter rye. The open-pollinated cultivar and all except one inbred line exhibited segregation for fertility restoration. Through further inbreeding, sub-lines of certain breeds were obtained which were homozygous for maintenance of sterility or for restoration of fertility. Environmental effects on the expression of fertility restoration were detected.

scholarly journals GENETIC RESEARCH OF QUANTITATIVE TRAITS OF INBRED LINES OF WINTER RYE (Secale cereale L.) IN DIALLEL CROSSINGS

2015 ◽  
Vol 50 (1) ◽  
pp. 75-84
Author(s):  
A.A. Goncharenko ◽  
◽  
S.V. Krahmalev ◽  
А.V. Мakarov ◽  
S.A. Yermakov ◽  
...  
Keyword(s):  
Secale Cereale ◽  
Quantitative Traits ◽  
Genetic Research ◽  
Inbred Lines ◽  
Winter Rye ◽  
Secale Cereale L

scholarly journals Assessment of similarity of inbred lines and F1 generations in collection of winter rye (Secale cereale L.) / Ocena podobieństwa linii wsobnych i pokolenia F1 w kolekcji żyta ozimego (Secale cereale L.)

2015 ◽  
Vol 26 (4) ◽  
pp. 30-34
Author(s):  
Agnieszka Pyza ◽  
Helena Kubicka-Matusiewicz ◽  
Dariusz Gozdowski
Keyword(s):  
Secale Cereale ◽  
Biological Diversity ◽  
Flag Leaf ◽  
Botanical Garden ◽  
Inbred Lines ◽  
Leaf Length ◽  
Stem Length ◽  
Leaf Width ◽  
Winter Rye ◽  
Secale Cereale L

Abstract Effective tools for evaluation of diversity in the collected gene resources of a given species are multivariate statistical methods. They provide information on phenotypic and genetic variability of collected material. The subjects of this study were nine inbred lines and three F1 generations of winter rye (Secale cereale L.), growing in experimental plots of the Polish Academy of Sciences Botanical Garden, Centre for Biological Diversity Conservation in Powsin. The evaluation was performed over the period of 3 years. Observations were made of the following traits: length of stem, length of spike, number of nodes in spike rachis, number of kernels per spike, weight of kernels per spike, weight of 1000 kernels, length of flag leaf, length of subflag leaf, length of third leaf, width of flag leaf, width of subflag leaf, width of third leaf, area of leaves per stem, number of stems per plant and area of leaves per plant. On the basis of cluster analysis and principal components analysis, two genetically homogeneous groups were identified. Mean values and standard deviations were calculated for each trait in each group and for all genotypes together. Multivariate distance matrix permitted identification of the most genetically similar and most distant forms.

scholarly journals Comparison of RAPD, ISSR and SSR markers in assessing genetic diversity among rye (Secale cereale L.) inbred lines

2010 ◽  
Vol 62 (1) ◽  
pp. 107-115 ◽  
Author(s):  
Beata Myśków ◽  
Paweł Milczarski ◽  
Piotr Masojć
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Secale Cereale ◽  
Simple Sequence Repeats ◽  
Random Amplified Polymorphic Dna ◽  
Correlation Coefficients ◽  
Inbred Lines ◽  
Winter Rye ◽  
Dna Primers ◽  
Simple Sequence

Comparison of RAPD, ISSR and SSR markers in assessing genetic diversity among rye (Secale cereale L.) inbred lines Forty eight inbred lines of winter rye, of various origin and pedigree, were analysed using 19 RAPD (random amplified polymorphic DNA) primers, 8 ISSR (inter-simple sequence repeats) primers and 13 SSR (simple sequence repeats) primer pairs. On the basis of particular marker types, there were created three separate dendrograms and one combined similarity tree, prepared on account of the whole data. Correlation coefficients for individual technique based on genetic similarity matrices were not significant. By comparing the GS data obtained on the basis of singular methods with collective matrix, it was observed that the highest correlation rate was for ISSR method (r=0.68). The utility of each marker technique was compared by using marker index MI. Diversity detecting index (DDT) was suggested in the paper, which may prove helpful in planning and comparing researches on phenetic relationships.

scholarly journals Study of the gene pool of the winter rye Secale cereale L. of the Republic of Belarus using microsatellite markers

2021 ◽  
Vol 66 (2) ◽  
pp. 215-222
Author(s):  
V. S. Mandrusova ◽  
I. S. Gordej ◽  
O. M. Lyusikov ◽  
V. E. Shimko ◽  
I. A. Gordej
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Gene Pool ◽  
Winter Rye ◽  
Interpopulation Variability ◽  
Content Index ◽  
Secale Cereale L ◽  
New Varieties ◽  
The Republic

In this work, the genetic diversity of the modern gene pool of the winter rye (S. cereal L.) of the Republic of Belarus from 20 actual breeding samples was investigated using 15 microsatellite (SSR) markers to develop divergent crossing combinations in breeding for heterosis. It was shown that the formed set of SSR markers is highly effective – the informational content index (PIC) varied from 0.50 to 0.83 and averaged 0.72. The most effective microsatellite markers (SCM28, SCM43, SCM101 and SCM102) were identified and can be successfully used to study the genetic diversity of rye. It has been established that the modern gene pool of the winter rye of the Republic of Belarus is generally characterized by fairly wide genetic diversity (interpopulation variability) – all collection samples are characterized by a unique allelic composition of the studied microsatellite loci. Based on investigation results, a hierarchical clustering dendrogram was constructed, which made it possible to determine the most genetically divergent combinations of crosses. The information obtained can be used for the development of an effective scheme allowing to develop new varieties and hybrids in the practical breeding of rye for heterosis.

THE GENETICS OF FERTILITY RESTORATION IN CYTOPLASMIC MALE-STERILE RYE

1979 ◽  
Vol 21 (3) ◽  
pp. 417-422 ◽  
Author(s):  
G. J. Scoles ◽  
L. E. Evans
Keyword(s):  
Secale Cereale ◽  
Fertility Restoration ◽  
Inbred Lines ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Cytoplasmic Male Sterile ◽  
Restorer Genes ◽  
Secale Cereale L ◽  
Partial Fertility

Three inbred lines of rye (Secale cereale L.) known to be capable of restoring fertility to a cytoplasmic male-sterile line were crossed with the sterile line. The proportions of male fertile, partially male fertile and male sterile plants in F2 and backcross progenies indicated that three dominant restorer genes were present in each line. These were designated Rf1, Rf2 and Rf3, their relative expressivity was Rf1>Rf2>Rf3. Expressivity was dependent upon environment. Partial fertility occurred when certain genotypes carried two of the three alleles as dominant, but was dependent upon genotype and environment.

Cell Wall Deposits in Winter Rye Secale cereale L. 'Puma' during Cold Acclimation

1982 ◽  
Vol 143 (4) ◽  
pp. 486-490 ◽  
Author(s):  
Marilyn Griffith ◽  
Gregory N. Brown
Keyword(s):  
Cell Wall ◽  
Cold Acclimation ◽  
Secale Cereale ◽  
Winter Rye ◽  
Secale Cereale L
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