scholarly journals Study of the gene pool of the winter rye Secale cereale L. of the Republic of Belarus using microsatellite markers

2021 ◽  
Vol 66 (2) ◽  
pp. 215-222
Author(s):  
V. S. Mandrusova ◽  
I. S. Gordej ◽  
O. M. Lyusikov ◽  
V. E. Shimko ◽  
I. A. Gordej
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Gene Pool ◽  
Winter Rye ◽  
Interpopulation Variability ◽  
Content Index ◽  
Secale Cereale L ◽  
New Varieties ◽  
The Republic

In this work, the genetic diversity of the modern gene pool of the winter rye (S. cereal L.) of the Republic of Belarus from 20 actual breeding samples was investigated using 15 microsatellite (SSR) markers to develop divergent crossing combinations in breeding for heterosis. It was shown that the formed set of SSR markers is highly effective – the informational content index (PIC) varied from 0.50 to 0.83 and averaged 0.72. The most effective microsatellite markers (SCM28, SCM43, SCM101 and SCM102) were identified and can be successfully used to study the genetic diversity of rye. It has been established that the modern gene pool of the winter rye of the Republic of Belarus is generally characterized by fairly wide genetic diversity (interpopulation variability) – all collection samples are characterized by a unique allelic composition of the studied microsatellite loci. Based on investigation results, a hierarchical clustering dendrogram was constructed, which made it possible to determine the most genetically divergent combinations of crosses. The information obtained can be used for the development of an effective scheme allowing to develop new varieties and hybrids in the practical breeding of rye for heterosis.

Analysis of Genetic Diversity in the Brassica napus L. Gene Pool Using SSR Markers

2005 ◽  
Vol 53 (4) ◽  
pp. 793-802 ◽  
Author(s):  
M. Hasan ◽  
F. Seyis ◽  
A. G. Badani ◽  
J. Pons-Kühnemann ◽  
W. Friedt ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Brassica Napus ◽  
Ssr Markers ◽  
Gene Pool ◽  
Brassica Napus L

scholarly journals Keragaman Genetik 96 Aksesi Plasma Nutfah Padi Berdasarkan 30 Marka SSR Terpaut Gen Pengatur Waktu Pembungaan (HD Genes)

2016 ◽  
Vol 7 (2) ◽  
pp. 76
Author(s):  
Dwinita Wikan Utami ◽  
Sutoro Sutoro ◽  
Nurul Hidayatun ◽  
Andari Risliawati ◽  
Ida Hanarida
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Heading Date ◽  
Germplasm Collection ◽  
Dna Fingerprint ◽  
Early Maturity ◽  
Plant Variety ◽  
Variety Protection ◽  
Genetic Analyzer

<p>Genetic Diversity of 96 Accession of Rice Germplasm<br />Using 30 SSR Markers Linked to Heading Date Genes (HD<br />Genes). Dwinita W. Utami, Sutoro, Nurul Hidayatun,<br />Andari Risliawati, and Ida Hanarida. Rice with early<br />maturity is one of an important genetic resources in rice<br />germplasm collection. Characterization and identification of<br />genetic diversity is an important issue for plant variety protection.<br />Molecular identification by microsatellite markers<br />using Genetic Analyzer enables resolve of this issue. The<br />objective of this research is to identify the genetic diversity of<br />96 rice accessions based on their specific DNA fingerprint<br />using microsatellite markers. A total of 96 accessions consisting<br />of a diverse variety of maturity classification were<br />genotyped with 30 SSR markers linked to HD genes which<br />spread out in 12 chromosomes of rice geneome. The total<br />297 alleles were detected indicated the level of marker<br />informativeness. RM5607 generated 7 allele with the size<br />range from 103 to 197 and the highest PIC at 0.90. RM3571<br />(linked to HD12 gene) has a significant value associated with<br />varieties which have early maturity trait. Clustering analysis<br />showed the cluster based on Sub Species genome background<br />and on early maturity trait.</p>

scholarly journals Molecular characterization and genetic diversity studies of Indian soybean (Glycine max (L.) Merr.) cultivars using SSR markers

2021 ◽  
Author(s):  
S. P. Jeevan Kumar ◽  
C. Susmita ◽  
K. V. Sripathy ◽  
Dinesh K. Agarwal ◽  
Govind Pal ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
Ssr Markers ◽  
Crop Yield ◽  
Gene Pool ◽  
Stress Factors ◽  
Soybean Cultivars ◽  
Genetic Base ◽  
Purity Analysis ◽  
Breeding Programmes

Abstract Background The genetic base of soybean cultivars in India has been reported to be extremely narrow, due to repeated use of few selected and elite genotypes as parents in the breeding programmes. This ultimately led to the reduction of genetic variability among existing soybean cultivars and stagnation in crop yield. Thus in order to enhance production and productivity of soybean, broadening of genetic base and exploring untapped valuable genetic diversity has become quite indispensable. This could be successfully accomplished through molecular characterization of soybean genotypes using various DNA based markers. Hence, an attempt was made to study the molecular divergence and relatedness among 29 genotypes of soybean using SSR markers. Methods and results A total of 35 SSR primers were deployed to study the genetic divergence among 29 genotypes of soybean. Among them, 14 primer pairs were found to be polymorphic producing a total of 34 polymorphic alleles; and the allele number for each locus ranged from two to four with an average of 2.43 alleles per primer pair. Polymorphic information content (PIC) values of SSRs ranged from 0.064 to 0.689 with an average of 0.331. The dendrogram constructed based on dissimilarity indices clustered the 29 genotypes into two major groups and four sub-groups. Similarly, principal coordinate analysis grouped the genotypes into four major groups that exactly corresponded to the clustering of genotypes among four sub-groups of dendrogram. Besides, the study has reported eight unique and two rare alleles that could be potentially utilized for genetic purity analysis and cultivar identification in soybean. Conclusion In the present investigation, two major clusters were reported and grouping of large number of genotypes in each cluster indicated high degree of genetic resemblance and narrow genetic base among the genotypes used in the study. With respect to the primers used in the study, the values of PIC and other related parameters revealed that the selected SSR markers are moderately informative and could be potentially utilized for diversity analysis of soybean. The clustering pattern of dendrogram constructed based on SSR loci profile displayed good agreement with the cultivar’s pedigree information. High level of genetic similarity observed among the genotypes from the present study necessitates the inclusion of wild relatives, land races and traditional cultivars in future soybean breeding programmes to widen the crop gene pool. Thus, hybridization among diverse gene pool could result in more heterotic combinations ultimately enhancing genetic gain, crop yield and resistance to various stress factors.

Genetic diversity of Colombian landraces of common bean as detected through the use of silver-stained and fluorescently labelled microsatellites

2011 ◽  
Vol 9 (01) ◽  
pp. 86-96 ◽  
Author(s):  
Lucy M. Díaz ◽  
Héctor F. Buendía ◽  
Myriam C. Duque ◽  
Matthew W. Blair
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
Common Bean ◽  
Gene Pool ◽  
Major Gene ◽  
Gene Pools ◽  
Silver Stain ◽  
Fluorescent Marker ◽  
Fluorescent Markers

Colombia, situated at the northern end of the Andes mountains of South America and in proximity to Central America, is an important centre of diversity for common bean (Phaseolus vulgarisL.) that has a mix of cultivated germplasm from both major gene pools (Andean and Mesoamerican) for the species. Microsatellites are a useful marker system for analyzing genetic diversity of this crop and can be analyzed with manual (silver-stain) or automated (ABI) detection systems and using unlabelled or fluorescently labelled markers, respectively. The objectives of this research were to evaluate the genetic diversity of 92 Colombian landraces and gene pool controls with 36 fluorescent and 30 non-fluorescent microsatellite markers and to determine the extent of introgression between the Andean and Mesoamerican gene pools for this germplasm. A comparison of fluorescentversusnon-fluorescent marker systems was performed with 14 loci, which were evaluated with both methods; the fluorescent markers were found to be more precise than the non-fluorescent markers in determining population structure. A combined analysis of 52 microsatellites using the 36 fluorescent markers and 16 non-overlapping, silver-stained markers produced an accurate population structure for the Andean gene pool that separated race Nueva Granada and race Peru genotypes and clearly identified introgression between these races and the gene pools. The results of this research are important for the application of microsatellite markers to diversity analysis in common bean and for the conservation of landraces in Colombia and neighbouring countries of Latin America, where similar germplasm exists and where gene pool or race mixtures also occur.

scholarly journals Evaluation of genetic diversity of honey bee in Ukraine analyzed by the SSR-markers

2020 ◽  
Vol 26 ◽  
pp. 56-60
Author(s):  
D. I. Hryhorchuk ◽  
A. M. Rabokon ◽  
A. S. PostovoitovA ◽  
N. M. Pirko ◽  
Ya. V. Pirko ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Honey Bee ◽  
Ssr Markers ◽  
Honey Bees ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Analysis Method ◽  
Ssr Analysis ◽  
Silver Nitrate Staining

Aim. The aim of the work was to analyze current genetic structure of honey bee populations in Ukraine that belong to different subspecies: A. meliffera meliffera, A. meliffera carnica, A. meliffera macedonica using microsatellite markers. Methods. SSR-analysis was used for evaluation of the honey bee polymorphism. Amplified fragments were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using silver nitrate staining. Results. The analysis of the sample of honey bees (workers and male-bees) collected from different regions of Ukraine was performed by using two SSR-markers (Ac011 and A007). In this sample reasonably high polymorphism was observed, especially for the SSR-marker A007. Conclusions. It was estimated that SSR-analysis method can be applied in molecular-genetic analysis of honey bees for evaluation of genetic diversity and cross-subspecies hybridization. Keywords: microsatellite markers, Apis meliffera, PIC (Polymorphism Information Content).

scholarly journals Molecular Assessment of Genetic Diversity among Egyptian Landraces of Wheat (Triticum aestivum L.) Using Microsatellite Markers

2020 ◽  
pp. 46-58 ◽  
Author(s):  
Ahmed Medhat Mohamed Al-Naggar ◽  
Mohamed Abd El-Maboud Abd El-Shafi ◽  
Mohamed Helmy El-Shal ◽  
Ali Hassan Anany
Keyword(s):  
Genetic Diversity ◽  
Triticum Aestivum ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Triticum Aestivum L ◽  
Genetic Progress ◽  
High Genetic Diversity ◽  
Breeding Programs ◽  
High Polymorphism Information Content ◽  
Wheat Landraces

To increase the genetic progress in wheat (Triticum aestivum L.) yield, breeders search for germplasm of high genetic diversity, one of them is the landraces. The present study aimed at evaluating genetic diversity of 20 Egyptian wheat landraces and two cultivars using microsatellite markers (SSRs). Ten SSR markers amplified a total of 27 alleles in the set of 22 wheat accessions, of which 23 alleles (85.2%) were polymorphic. The majority of the markers showed high polymorphism information content (PIC) values (0.67-0.94), indicating the diverse nature of the wheat accessions and/or highly informative SSR markers used in this study. The genotyping data of the SSR markers were used to assess genetic variation in the wheat accessions by dendrogram. The highest genetic distance was found between G21 (Sakha 64; an Egyptian cultivar) and the landrace accession No. 9120 (G11). These two genotypes could be used as parents in a hybridization program followed by selection in the segregating generations, to identify some transgressive segregates of higher grain yield than both parents. The clustering assigned the wheat genotypes into four groups based on SSR markers. The results showed that the studied SSR markers, provided sufficient polymorphism and reproducible fingerprinting profiles for evaluating genetic diversity of wheat landraces. The analyzed wheat landraces showed a good level of genetic diversity at the molecular level. Molecular variation evaluated in this study of wheat landraces can be useful in traditional and molecular breeding programs.

Genetic diversity of wheat gene pool of recurrent selection assessed by microsatellite markers and morphological traits

Euphytica ◽  
2006 ◽  
Vol 155 (1-2) ◽  
pp. 249-258 ◽  
Author(s):  
Jiancheng Liu ◽  
Like Liu ◽  
Ning Hou ◽  
Aimin Zhang ◽  
Chunguang Liu
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Gene Pool ◽  
Morphological Traits ◽  
Recurrent Selection ◽  
Wheat Gene

scholarly journals Genetic Diversity and Structure of Pinus densiflora Siebold & Zucc. Populations in Republic of Korea Based on Microsatellite Markers

Forests ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 750
Author(s):  
Ji-Young Ahn ◽  
Jei-Wan Lee ◽  
Kyung-Nak Hong
Keyword(s):  
Genetic Diversity ◽  
Genetic Differentiation ◽  
Microsatellite Markers ◽  
Environmental Changes ◽  
Pinus Densiflora ◽  
Republic Of Korea ◽  
Conifer Species ◽  
Principal Coordinates ◽  
Genetic Diversity And Structure ◽  
The Republic

Pinus densiflora Siebold & Zucc. is a widely distributed conifer species in the Republic of Korea with economic and ecologic importance. However, P. densiflora is negatively influenced by various factors, such as forest fires, clearing of large numbers of trees infected with Pinus disease, and dieback. We aimed to use microsatellite markers to estimate the genetic diversity, differentiation, and structure of P. densiflora populations in the Republic of Korea. A total of 1844 samples from 60 populations were evaluated using 11 polymorphic microsatellite markers. The observed heterozygosity and expected heterozygosity were 0.652 and 0.673, respectively. The mean genetic differentiation among the populations was 0.013. Moreover, P. densiflora showed high genetic diversity and low genetic differentiation compared with conifer species, including Pinus species with similar life histories. Principal coordinates analysis and Bayesian clustering showed that P. densiflora has a weak geographical structure. The P. densiflora population at Mt. Halla, Jeju Island, showed the lowest genetic diversity and significant genetic differentiation compared with other mainland populations due to genetic drift and restricted gene flow. These findings can be useful for designing new conservation, management, and breeding strategies for P. densiflora populations in response to future environmental changes.

scholarly journals Characterization of Genetic Diversity of Stone Fruit Rootstocks Used in Chile by Means of Microsatellite Markers

2012 ◽  
Vol 137 (5) ◽  
pp. 302-310 ◽  
Author(s):  
María José Arismendi ◽  
Patricio Hinrichsen ◽  
Ruben Almada ◽  
Paula Pimentel ◽  
Manuel Pinto ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Stone Fruit ◽  
Identification System ◽  
Expected Heterozygosity ◽  
Commercially Important ◽  
Taxonomic Groups ◽  
Simple Sequence

Stone fruit (Prunus L.) production in Chile covers ≈43,000 ha and includes a wide variety of soils and climates requiring a large diversity of rootstocks. The most commercially important rootstock cultivars are 26 genotypes from three different taxonomic groups belonging to the subgenera Amygdalus (L.) Benth. Hook. (peach group), Prunus Focke [= Prunophora (Neck.)] Focke (plum group), and Cerasus (Adans.) Focke (cherry group) with eight, seven, and 10 individuals, respectively. To determine their genetic diversity, characterization by microsatellite markers [simple sequence repeat (SSR)] was conducted. Of a total of 20 SSR markers evaluated, 12 generated amplified products that were consistent in the three taxonomic groups. The number of alleles per marker ranged from 18 for PSM-3 to four in CPPCT-002. Clustering analysis, by both traditional hierarchical and model-based approaches, indicate that all genotypes are clustered in their respective taxonomic groups, including the interspecific hybrids. Genetic diversity, measured as the average distances (expected heterozygosity) between individuals in the same cluster, was higher in Cerasus (0.78) followed by Prunus (0.72) and Amygdalus (0.64). Total number of alleles observed was 133, of which 14, 33, and 35 from six, 10, and 10 loci were unique for the peach, plum, and cherry rootstock groups, respectively. Alleles shared among peach/plum, plum/cherry, and peach/cherry rootstock genotypes were 13, 14, and 18 from nine, seven, and seven loci, respectively. Only six alleles from five loci were common to the three taxonomic groups. In addition, to develop a rootstock identification system based on SSR markers, a minimum set of three markers (PMS-3, BPPCT-037, and BPPCT-036) able to differentiate the 26 genotypes was identified. This study is the first step toward establishing a stone fruit rootstock breeding program in Chile.

scholarly journals Genetic diversity of different late blight responsive Indian potato cultivars as revealed by SSR markers

2020 ◽  
Vol 80 (02) ◽  
Author(s):  
Virupaksh U. Patil ◽  
G. Vanishree ◽  
Leena Saikia ◽  
Vinay Bhardwaj ◽  
S. K. Chakrabarti
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Late Blight ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Potato Cultivars ◽  
Indian Potato Cultivars ◽  
Moderately Resistant ◽  
Resistant Genotypes ◽  
Very High

Forty seven resistant and susceptible to late blight Indian potato cultivars were used to assess genetic diversity present among them. A set of 24 microsatellite markers applied which generated 193 alleles with an average of 7.04 per loci and 4.10 per genotype. Eight alleles were specific to resistant cultivars, whereas 11 were specific to susceptible. Genetic diversity among the moderately resistant genotypes was found to be higher than the susceptible and resistant ones. The cluster analysis grouped the cultivars into 2 main and 4 sub clusters with late blight resistant genotypes distributed across the clusters. The diverisity among the genotypes was very high; however, diversity within groups viz., resistant, moderately resistant and susceptible was found to be very low (6%). The findings of the study would be of great help to the breeders in selecting genotypes for developing improved varieties of potato.

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