Leaf and neck blast resistance reaction in tropical rice lines under green house condition

Rice (Oryza sativa L.) is one of the most important food crops from which nearly one third of world's population derives its principal source of calories. The experiment was conducted to discover the impending genotypes suitable for hilly situation of Karnataka. The experimental materials were chosen or selected based on significant superiority or on par yield over the local check variety, i.e., Tunga in the AICRIP investigational trials of paddy. The chosen entries were evaluated for the three years in the station trial at the Agricultural and Horticultural Research Station, Ponnampet in randomized complete block design with two replications during 2016, 2017 and 2018 kharif seasons. Third year Multi Location Trial (MLT) was also conducted at AHRS, Ponnampet and ZAHRS, Mudigere. The pooled data across the years in the station trial revealed that the entry IET-24451 recorded the utmost grain yield (6196 kg/ha) among the entries and gave up 36 per cent increased grain yield over the Tunga and 17 per cent increased grain yield over the KPR-1. The pooled data of MLT across the locations revealed that the entry IET-25281 recorded the utmost grain yield of 6076 kg/ha with 11 per cent increased grain yield over the Tunga and 13 per cent increased grain yield over the KPR-1. The combined pooled data of station trial across the years and MLT trial across locations revealed that the entry, IET-24451 of 6135 kg/ha recorded the maximum grain yield followed by IET-25281 (5970 kg/ha) among the entries. The entry IET-24451 recorded 22 per cent increased grain yield over the Tunga and 15 per cent increased grain yield over the KPR-1, whereas the entry IET-25281 recorded 19 per cent increased grain yield over the Tunga and 12 per cent increased grain yield over the KPR-1 and both the entries IET-24451 and IET-25281 recorded the leaf and neck blast resistance reaction in the Uniform Blast Nursery Screening Pattern. Hence, the entry IET-24451 was recommended for on-farm trial due to its blast resistance trait and yield superiority across the years and the locations.

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Ketahanan Galur-Galur Padi Pup1 terhadap Penyakit Blas

Jurnal Penelitian Pertanian Tanaman Pangan ◽

10.21082/jpptp.v34n1.2015.p29-36 ◽

2015 ◽

Vol 34 (1) ◽

pp. 29 ◽

Cited By ~ 2

Author(s):

Tasliah Tasliah ◽

Joko Prasetiyono ◽

Tintin Suhartini ◽

Ida Hanarida Soemantri

Keyword(s):

Molecular Analysis ◽

Gene Locus ◽

Upland Rice ◽

Blast Resistance ◽

Fungal Growth ◽

Blast Disease ◽

Fertilizer Efficiency ◽

Research Station ◽

Fungus Infection ◽

Green House

<p>Blast is one of major disease on the upland rice in Indonesia. Upland rice lines derived from Kasalath and NILC443 crosses, containing Pup1 gen locus had been developed and evaluated for P fertilizer efficiency. Those lines would be evaluated for blast resistance, due to the fact that Pup1 locus contains genes involved in plant defend mechanism to disease, including blast disease. The BC2F5 plants derived from six crosses (DK, DN, SK, SN, BK, BN) were used in this research. Responses to blast disease in the green house were evaluated at ICABIOGRAD Bogor from March to April 2011, using combination of three blast races (race 173, 033, and 133). The response to blast disease in the field was evaluated at Taman Bogo Research Station, Lampung, and at farmer’s field in Cikeusal Village, Banten, from January to April 2011. Molecular analysis to trace Pup1 gene locus was conducted at the Molecular Biology Laboratory, using specific primer K20-2, from January to August 2013. Based on the molecular analysis all Pup1 lines showed homozygoes alleles, except the heterozygoes alleles on SK7, SK8, SK15, SK16, BN8 line, which were then not included in the next planting. The responses to blast at greenhouse among lines varied, but the Pup1 lines were mostly at level of moderate resistan (AT). Based on the result from the field experiment, most of Pup1 lines were resistance, however the susceptible check plant (Kencana Bali) did not show blast fungus infection. Differences of the result might be due to the blast testing at the green house which was more favorable for blast fungal growth. The effect of Pup1 gene locus showed clearly on resistance of plants obtained from Situ Bagendit cross, where Situ Bagendit was susceptible and does not contain the Pup1 locus. Additional of Pup1 locus in Situ Bagendit genome had increased the degree of resistant to blast.</p>

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Reaction of Different Rice Lines Against Leaf and Neck Blast under Field Condition Of Chitwan Valley

Journal of the Institute of Agriculture and Animal Science ◽

10.3126/jiaas.v27i0.693 ◽

2006 ◽

Vol 27 ◽

pp. 37-44 ◽

Cited By ~ 1

Author(s):

KD Puri ◽

SM Shrestha ◽

KD Joshi ◽

GB KC

Keyword(s):

Field Condition ◽

Rice Blast ◽

Blast Resistance ◽

Blast Disease ◽

Resistant Variety ◽

Pyricularia Grisea ◽

Leaf Blast ◽

Neck Blast ◽

Chitwan Valley ◽

Moderately Resistant

The severity of the rice blast disease (Pyricularia grisea) of both leaf and neck varies with different environment and it becomes destructive under favorable condition. The leaf and neck blast resistance and susceptible interaction of 30 different tropical rice lines were evaluated under low-, mid- and up-land conditions of Chitwan district and classified on the basis of disease severity with respect to susceptible check, Masuli. Of them, 5, 10, 12 and 3 rice lines were resistant to leaf blast, moderately resistant, moderately susceptible susceptible, respectively. Similarly, for the neck blast nine lines were resistant, thirteen moderately resistant, seven moderately susceptible and one was susceptible. The progenies from Masuli/MT4 had the highest leaf and neck blast susceptible reaction, while the most of progenies from IPB (Irradiated Pusa Basmati), KalinghaIII_IR64, Radha 32_ KIII and Masuli_IR64 were resistant, and the most promising sources against leaf and neck blast resistance. Therefore, the progenies from these parents can be used in breeding the resistant variety. Key words: Pyricularia grisea, resistance, rice lines J. Inst. Agric. Anim. Sci. 27:37-44 (2006)

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Resistance in Rice Breeding Lines to the Blast Fungus in Nepal

Nepal Agriculture Research Journal ◽

10.3126/narj.v6i0.3344 ◽

1970 ◽

Vol 6 ◽

pp. 49-56 ◽

Cited By ~ 2

Author(s):

Bedanand Chaudhary ◽

Sundar M Shrestha ◽

Ram C Sharma

Keyword(s):

Field Condition ◽

Blast Resistance ◽

Quantitative Resistance ◽

Rice Breeding ◽

Breeding Lines ◽

Research Journal ◽

Neck Blast ◽

Upland Field ◽

Major Resistance Genes ◽

Greenhouse Assay

A total of 36 rice breeding lines including checks were evaluated for resistance to blast atRampur during 2000-2001. The experiments were conducted under both field condition andgreenhouse inoculated condition. Qualitative resistance in rice to blast was assessed based onlesion type, whereas quantitative resistance was assessed based on area under disease progresscurve (AUDPC) in the upland field condition. The number of sporulating lesions and the numberof leaves with at least one sporulating lesion per plant were considered as measures forevaluation of quantitative resistance in the greenhouse assay. The lesion type, neck blastpercentage and AUDPC data suggest that most of the rice lines possess higher level of resistanceto leaf and neck blast. The rice lines varied for the number of sporulating lesions and the numberof leaves with sporulating lesion per plant. Some lines were incompatible to virulent blastisolates, showing major resistance genes. NR 1558, NR 601-1-1-9, BW306-2 and CN 836-3-10were promising lines for quantitative resistance to both leaf and neck blast. Radha 12, Sabitri,Janaki possess higher level of quantitative resistance to blast, hence these could be promoted forcultivation in blast-prone environments. These genotypes could also be utilized as donor parentsfor breeding durable blast resistant varieties. The most virulent blast isolate could be used forevaluation of both qualitative and quantitative resistance to blast in early generation in thegreenhouse so that workload could be cut down in future works.Key words: Blast resistance; field condition; greenhouse assay; Pyricularia grisea; rice linesDOI: 10.3126/narj.v6i0.3344Nepal Agriculture Research Journal Vol.6 2005 pp.49-56

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