scholarly journals Stripe rust resistance and genes in Chinese wheat cultivars and breeding lines

Euphytica ◽  
2013 ◽  
Vol 196 (2) ◽  
pp. 271-284 ◽  
Author(s):  
Qing-Dong Zeng ◽  
De-Jun Han ◽  
Qi-Lin Wang ◽  
Feng-Ping Yuan ◽  
Jian-Hui Wu ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Stripe Rust Resistance ◽  
Wheat Cultivars ◽  
Breeding Lines ◽  
Chinese Wheat

Genome-wide Wheat 55K SNP-based Mapping of Stripe Rust Resistance Loci in Wheat Cultivar Shaannong 33 and Their Alleles Frequencies in Current Chinese Wheat Cultivars and Breeding Lines

Plant Disease ◽  
2020 ◽  
Author(s):  
Shuo Huang ◽  
Shengjie Liu ◽  
Yibo Zhang ◽  
Yanzhou Xie ◽  
Xiaoting Wang ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Wheat Cultivar ◽  
Stripe Rust Resistance ◽  
Wheat Cultivars ◽  
Seedling Resistance ◽  
Resistant Parent ◽  
Breeding Lines ◽  
Chinese Wheat

Wheat cultivar Shannong 33 (SN33) has remained highly resistant to stripe rust in the field since its release in 2009. To unravel the genetic architecture of stripe rust resistance, seedlings of 161 recombinant inbred lines (RILs) from the cross Avocet S × SN33 were evaluated with two isolates (PST-Lab.1 and PST-Lab.2) of the stripe rust pathogen (Puccinia striiformis f. sp. tritici) in the greenhouse, and the RILs were evaluated in naturally and/or artificially inoculated field sites during two cropping seasons. The RILs and parents were genotyped with the wheat 55K single nucleotide polymorphism (SNP) array. Three genomic regions conferring seedling resistance were mapped on chromosomes 1DS, 2AS, and 3DS, and four consistent quantitative trait loci (QTL) for adult-plant resistance (APR) were detected on 1BL, 2AS, 3DL, and 6BS. The 2AS locus conferring all-stage resistance was identified as the resistant gene Yr17 located on 2NS translocation. The QTL identified on 1BL and 6BS likely correspond to Yr29 and Yr78, respectively. An APR QTL on 3DL explaining 5.8–12.2% of the phenotypic variation is likely to be new. Molecular marker detection assays with the 2NS segment (Yr17), Yr29, Yr78, and QYrsn.nwafu-3DL on a panel of 420 current Chinese wheat cultivars and breeding lines indicated that these genes were present in 11.4%, 7.6%, 14.8%, and 7.4% entries, respectively. The interactions among these genes/QTL were additive suggesting their potential value in enhancing stripe rust resistance breeding materials as observed in the resistant parent. In addition, we also identified two leaf necrosis genes, Ne1 and Ne2, however, the F1 plants from cross Avocet S × SN33 survived indicating that SN33 probably has another allele of Ne1 which allows to harvest seeds.

Distribution, Frequency and Variation of Stripe Rust Resistance Loci Yr10, Lr34/Yr18 and Yr36 in Chinese Wheat Cultivars

2012 ◽  
Vol 39 (11) ◽  
pp. 587-592 ◽  
Author(s):  
Cuiling Yuan ◽  
Hui Jiang ◽  
Honggang Wang ◽  
Kun Li ◽  
Heng Tang ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Stripe Rust Resistance ◽  
Wheat Cultivars ◽  
Chinese Wheat ◽  
Distribution Frequency

Identification of Stripe Rust Resistance Loci in U.S. Spring Wheat Cultivars and Breeding Lines Using Genome-Wide Association Mapping and Yr Gene Markers

Plant Disease ◽  
2020 ◽  
Vol 104 (8) ◽  
pp. 2181-2192 ◽  
Author(s):  
Lu Liu ◽  
Meinan Wang ◽  
Zhiwu Zhang ◽  
Deven R. See ◽  
Xianming Chen
Keyword(s):  
Stripe Rust ◽  
Spring Wheat ◽  
Resistance Genes ◽  
Rust Resistance ◽  
The United States ◽  
Genome Wide Association ◽  
Stripe Rust Resistance ◽  
Wheat Cultivars ◽  
Breeding Lines ◽  
Genome Wide

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), poses a major threat to wheat production worldwide, especially in the United States. To identify loci for effective stripe rust resistance in U.S. wheat, a genome-wide association study (GWAS) was conducted using a panel of 616 spring wheat cultivars and breeding lines. The accessions in this panel were phenotyped for stripe rust response in the greenhouse at seedling stage with five predominant and highly virulent races of Pst and in different field environments at adult-plant stage in 2017 and 2018. In total, 2,029 single-nucleotide polymorphism markers that cover the whole genome were generated with genotyping by multiplexed sequencing and used in GWAS. In addition, 23 markers of previously reported resistance genes or quantitative trait loci (QTLs) were used to genotype the population. This spring panel was grouped into three subpopulations based on principal component analysis. A total of 37 genes or QTLs including 10 potentially new QTLs for resistance to stripe rust were detected by GWAS and linked marker tests. The frequencies of the resistance genes or QTLs in various nurseries were determined, indicating different intensities of these genes or QTLs used in breeding programs of different regions. These resistance loci and the information on their markers, effectiveness, and distributions should be useful for improving stripe rust resistance in wheat cultivars.

Exome Sequencing from Bulked Segregant Analysis Identifies a Gene for All Stage Resistance to Stripe Rust on Chromosome 1AL in Chinese Wheat Landrace Xiaohemai

Plant Disease ◽  
2021 ◽  
Author(s):  
Yunfeng Jiang ◽  
Luyao Duan ◽  
Fangnian Guan ◽  
Fangjie Yao ◽  
Li Long ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Bulked Segregant Analysis ◽  
Stripe Rust Resistance ◽  
Exome Capture ◽  
Phenotypic Variance ◽  
Wheat Cultivars ◽  
Wheat Landrace ◽  
Chinese Wheat ◽  
Kasp Markers

Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is one of the most destructive diseases of wheat. Identifying novel resistance genes applicable for developing disease resistant cultivars is important for the sustainable control of wheat stripe rust. Chinese wheat landrace Xiaohemai (XHM) is an elite germplasm line with all-stage resistance (ASR) effective against predominant Chinese Pst races. In this study, we performed a bulked segregant analysis coupled with exome capture sequencing (BSE-seq) to identify a candidate genomic region strongly associated with stripe rust resistance on chromosome 1AL in 173 F2:3 lines derived from cross XHM × Avocet S. The gene, designated as YrXH-1AL, was validated by a conventional quantitative trait locus analysis using newly developed Kompetitive allele-specific PCR (KASP) markers, explaining up to 48.50% of the phenotypic variance. By testing a secondary mapping population comprising 144 lines from the same cross at the seedling stage with prevalent Pst race CYR34, YrXH-1AL was identified as a single Mendelian factor in a 1.5 cM interval flanked by KASP markers KP1A_484.33 and KP1A_490.09. This region corresponded to a 5.76 Mb genomic interval on Chinese Spring chromosome 1AL. Furthermore, two co-segregating KASP markers showed high polymorphisms among 130 Chinese wheat cultivars and could be used for marker-assisted selection. Because no other Yr genes for ASR that originated from common wheat have been detected on chromosome 1AL, YrXH-1AL is likely a novel gene that can be incorporated into modern breeding materials to develop wheat cultivars with enhanced stripe rust resistance.

scholarly journals Genome-wide association analysis of stripe rust resistance in modern Chinese wheat

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Mengjie Jia ◽  
Lijun Yang ◽  
Wei Zhang ◽  
Garry Rosewarne ◽  
Junhui Li ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Genome Wide Association ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Amplification Refractory Mutation System ◽  
Post Inoculation ◽  
Genome Wide ◽  
A Genome ◽  
Chinese Wheat

Abstract Background Stripe rust (yellow rust) is a significant disease for bread wheat (Triticum aestivum L.) worldwide. A genome-wide association study was conducted on 240 Chinese wheat cultivars and elite lines genotyped with the wheat 90 K single nucleotide polymorphism (SNP) arrays to decipher the genetic architecture of stripe rust resistance in Chinese germplasm. Results Stripe rust resistance was evaluated at the adult plant stage in Pixian and Xindu in Sichuan province in the 2015–2016 cropping season, and in Wuhan in Hubei province in the 2013–2014, 2016–2017 and 2018–2019 cropping seasons. Twelve stable loci for stripe rust resistance were identified by GWAS using TASSEL and GAPIT software. These loci were distributed on chromosomes 1B, 1D, 2A, 2B, 3A, 3B, 4B (3), 4D, 6D, and 7B and explained 3.6 to 10.3% of the phenotypic variation. Six of the loci corresponded with previously reported genes/QTLs, including Sr2/Yr30/Lr27, while the other six (QYr.hbaas-1BS, QYr.hbaas-2BL, QYr.hbaas-3AL, QYr.hbaas-4BL.3, QYr.hbaas-4DL, and QYr.hbaas-6DS) are probably novel. The results suggest high genetic diversity for stripe rust resistance in this population. The resistance alleles of QYr.hbaas-2AS, QYr.hbaas-3BS, QYr.hbaas-4DL, and QYr.hbaas-7BL were rare in the present panel, indicating their potential use in breeding for stripe rust resistance in China. Eleven penta-primer amplification refractory mutation system (PARMS) markers were developed from SNPs significantly associated with seven mapped QTLs. Twenty-seven genes were predicted for mapped QTLs. Six of them were considered as candidates for their high relative expression levels post-inoculation. Conclusion The resistant germplasm, mapped QTLs, and PARMS markers developed in this study are resources for enhancing stripe rust resistance in wheat breeding.

scholarly journals Evaluation of Stripe Rust Resistance in Hungarian Winter Wheat Cultivars in China

2019 ◽  
Vol 47 (4) ◽  
pp. 636-644
Author(s):  
D. Huang ◽  
H. Zhang ◽  
M. Tar ◽  
Y. Zhang ◽  
F. Ni ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Stripe Rust Resistance ◽  
Wheat Cultivars ◽  
Winter Wheat Cultivars

Molecular Mapping of a Novel QTL Conferring Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace ‘Guangtoumai’

Plant Disease ◽  
2020 ◽  
Author(s):  
Yu Wu ◽  
Yuqi Wang ◽  
Fangjie Yao ◽  
Li Long ◽  
Jing Li ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Plant Resistance ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Major Locus ◽  
Dcaps Marker ◽  
Chinese Wheat

Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat in the world. Chinese wheat landrace ‘Guangtoumai’ (GTM) exhibited a high-level resistance against predominant Pst races in China at the adult-plant stage. The objective of this research was to identify and map the major locus/loci for stripe rust resistance in GTM. A set of 212 recombinant inbred lines (RILs) was developed from a cross between GTM and Avocet S (AvS). The parents and RILs were evaluated in three field tests (2018, 2019, and 2020 at Chongzhou, Sichuan) with the currently predominant Pst races for final disease severity (FDS) and genotyped with the Wheat 55K SNP array to construct a genetic map with 1,031 SNP markers. A major locus, named QYr.GTM-5DL, was detected on chromosome 5DL in GTM. The locus was mapped in a 2.75 cM interval flanked by SNP markers AX-109855976 and AX-109453419, explaining up to 44.4% of the total phenotypic variation. Since no known Yr genes have been reported on chromosome 5DL, QYr.GTM-5DL is very likely a novel adult plant resistance (APR) locus. Haplotype analysis revealed that the resistance allele displayed enhanced levels of stripe rust resistance and is likely present in 5.3% of the 247 surveyed Chinese wheat landraces. The derived cleaved amplified polymorphic sequence (dCAPS) marker dCAPS-5722, converted from a SNP marker tightly linked to QYr.GTM-5DL with 0.3 cM, was validated on a subset of RILs and 48 commercial wheat cultivars developed in Sichuan. The results indicated that QYr.GTM-5DL with its linked dCAPS marker could be used in marker-assisted selection to improve stripe rust resistance in breeding programs, and this QTL will provide new and possibly durable resistance to stripe rust.

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