Haematology and leucocyte enzyme cytochemistry of a threatened yellow catfish Horabagrus brachysoma (Gunther 1864)

Cisplatin (cis-dichlorodiammineplatinum(II)) a potent antitumor agent is now available for the treatment of testicular and ovarian cancers. It is however, not free from its serious side effects including nephrotoxicity, gastro intestinal toxicity, myelosuppression, and ototoxicity. Here we now report that the drug produces peculiar bloating of the stomach in rats and induces acute ulceration.Wistar-derived rats weighing 200-250 g were administered cisplatin(9 mg/kg) ip as a single dose in 0.15 M NaCl. After 3 days the animals were sacrificed by decapitation. The stomachs were removed, the contents analyzed for pepsin and acidity. The inner surface was examined with a dissecting microscope after a moderate stretching for ulcers. Affected areas were fixed and processed for routine electron microscopy and enzyme cytochemistry.The drug treated animals kept on food and water consistently showed bloating and lesions (Fig. 1) with a frequency of 6-70 ulcers in the rumen section of the stomachs.

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Integrated morphometrical and electron energy loss image analysis in cytochemistry

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100153981 ◽

1989 ◽

Vol 47 ◽

pp. 402-403

Author(s):

W.C. de Bruijn ◽

A.A.W. de Jong ◽

C.W.J. Sorber

Keyword(s):

Image Analysis ◽

Acid Phosphatase ◽

Chemical Analysis ◽

Active Form ◽

List Type ◽

Type Number ◽

Enzyme Cytochemistry ◽

Related Data ◽

Endogenous Peroxidase ◽

Electron Energy Loss

One aspect of enzyme cytochemistry is, whether all macrophage lysosomal hydrolytical enzymes are present in an active form, or are activated upon stimulation. Integrated morphometrical and chemical analysis has been chosen as a tool to illucidate that cytochemical problem. Mouse peritoneal resident macrophages have been used as a model for this complicated integration of morphometrical and element-related data. Only aldehyde-fixed cells were treated with three cytochemical reactions to detect different enzyme activities within one cell (for details see [1,2]). The enzyme-related precipitates anticipated to be differentiated, were:(1).lysosomal barium and sulphur from aryl sulphatase activity,(2).lysosomal cerium and phosphate from acid phosphatase activity and(3).platinum/di-amino-benzidine( D A B) complex from endogenous peroxidase activity.

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High time resolution enzyme cytochemistry of rod outer segment

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100161953 ◽

1990 ◽

Vol 48 (3) ◽

pp. 878-879

Author(s):

Takuma Saito ◽

Toshihiro Takizawa

Keyword(s):

Enzyme Activities ◽

Outer Segment ◽

Rapid Change ◽

High Time Resolution ◽

Visual Cell ◽

Enzyme Cytochemistry ◽

Activation Effect ◽

Rod Outer Segment ◽

Enzymatic Cascades ◽

Rapid Drop

Cells and tissues live on a number of dynamic metabolic pathways, which are made up of sequential enzymatic cascades.Recent biochemical and physiological studies of vision research showed the importance of cGMP metabolism in the rod outer segment of visual cell, indicat ing that the photon activated rhodopsin exerts activation effect on the GTP binding protein, transducin, and this act ivated transducin further activates phosphodiesterase (PDEase) to result in a rapid drop in cGMP concentration in the cytoplasm of rod outer segment. This rapid drop of cGMP concentration exerts to close the ion channel on the plasma membrane and to stop of inward current brings hyperpolarization and evokes an action potential.These sequential change of enzyme activities, known as cGMP cascade, proceeds quite rapidly within msec order. Such a rapid change of enzyme activities, such as PDEase in rod outer segment, was not a matter of conventional histochemical invest igations.

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