Identification of proteins differentially expressed in the gills of grass carp (Ctenopharyngodon idella) after hypoxic stress by two-dimensional gel electrophoresis analysis

2019 ◽  
Vol 45 (2) ◽  
pp. 743-752 ◽  
Author(s):  
Zhan-Ning Xu ◽  
Guo-Dong Zheng ◽  
Cheng-Bin Wu ◽  
Xia-Yun Jiang ◽  
Shu-Ming Zou
Keyword(s):  
Grass Carp ◽  
Gel Electrophoresis ◽  
Differentially Expressed ◽  
Ctenopharyngodon Idella ◽  
Hypoxic Stress ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Grass Carp Ctenopharyngodon Idella

Characterization of the Microbiota in Air- or Vacuum-Packed Crisp Grass Carp (Ctenopharyngodon idella C. et V.) Fillets by 16S rRNA PCR–Denaturing Gradient Gel Electrophoresis and High-Throughput Sequencing

2018 ◽  
Vol 81 (6) ◽  
pp. 1022-1029 ◽  
Author(s):  
ZIQIANG PAN ◽  
LIN LI ◽  
ZHIHUA SHEN ◽  
YAN CHEN ◽  
MEI LI
Keyword(s):  
16S Rrna ◽  
High Throughput ◽  
Grass Carp ◽  
Gel Electrophoresis ◽  
High Throughput Sequencing ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Ctenopharyngodon Idella ◽  
Chilled Storage ◽  
Grass Carp Ctenopharyngodon Idella ◽  
Gradient Gel Electrophoresis

ABSTRACT The microbial communities in air- and vacuum-packed crisp grass carp (Ctenopharyngodon idella C. et V.) fillets have not been characterized during chilled storage. High-throughput sequencing of bacterial 16S rRNA has now revealed that the bacterial community in fresh fillets is diverse and distinct from that in spoiled samples. The predominant phylum was Proteobacteria, and 66 genera were identified. In fresh fillets, the most abundant genera were Acinetobacter (53.3%), Wautersiella (6.3%), unclassified Alcaligenaceae (4.4%), Stenotrophomonas (3.8%), unclassified Enterobacteriaceae (3.8%), and Enhydrobacter (3.6%). These genera diminished during chilled storage and sometimes disappeared. At the end of storage, Aeromonas and Pseudomonas were the most abundant. Similar results were obtained by PCR–denaturing gradient gel electrophoresis. These data provide detailed insight into the evolving bacterial communities in air- and vacuum-packed crisp grass carp fillets during storage, revealing Aeromonas and Pseudomonas as major spoilage organisms. These data may be useful for improvement of crisp grass carp quality and shelf life during chilled storage.

Diversity of Glycoprotein Deficiencies in Glanzmann’s Thrombasthenia

1985 ◽  
Vol 54 (03) ◽  
pp. 626-629 ◽  
Author(s):  
M Meyer ◽  
F H Herrmann
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Type Ii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Structural Variant ◽  
Glanzmann’S Thrombasthenia ◽  
Crossed Immunoelectrophoresis ◽  
Glanzmann's Thrombasthenia ◽  
Platelet Proteins

SummaryThe platelet proteins of 9 thrombasthenic patients from 7 families were analysed by high resolution two-dimensional gel electrophoresis (HR-2DE) and crossed immunoelectrophoresis (CIE). In 7 patients both glycoproteins (GPs) IIb and Ilia were absent or reduced to roughly the same extent. In two related patients only a trace of GP Ilb-IIIa complex was detected in CIE, but HR-2DE revealed a glycopeptide in the position of GP Ilia in an amount comparable to type II thrombasthenia. This GP Ilia-like component was neither recognized normally by anti-GP Ilb-IIIa antibodies nor labeled by surface iodination. In unreduced-reduced two-dimensional gel electrophoresis two components were observed in the region of GP Ilia. The assumption of a structural variant of GP Ilia in the two related patients is discussed.

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