Phenotypic characterization of diverse Bambara groundnut (Vigna subterranea [L.] Verdc.) germplasm collections through seed morphology

2016 ◽  
Vol 63 (5) ◽  
pp. 889-899 ◽  
Author(s):  
M. S. Mohammed ◽  
H. A. Shimelis ◽  
M. D. Laing
Keyword(s):  
Seed Morphology ◽  
Bambara Groundnut ◽  
Phenotypic Characterization ◽  
Vigna Subterranea ◽  
Germplasm Collections

scholarly journals Molecular Characterization of a Large Diversity of Bambara Groundnut Potyviruses (<i>Vigna subterranea</i> (L.) Verdc.) in Burkina Faso

2019 ◽  
Vol 10 (09) ◽  
pp. 270-281
Author(s):  
Aboubié Elisabeth Zongo ◽  
Bouma James Néya ◽  
Essowé Palanga ◽  
Drissa Sérémé ◽  
Moustapha Koala ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Molecular Characterization ◽  
Bambara Groundnut ◽  
Vigna Subterranea

scholarly journals Investigation of obsolete diversity of rye (Secale cereale L.) using multiplexed SSR fingerprinting and evaluation of agronomic traits

2020 ◽  
Vol 61 (4) ◽  
pp. 513-529
Author(s):  
Malgorzata Targonska-Karasek ◽  
Maja Boczkowska ◽  
Wieslaw Podyma ◽  
Małgorzata Pasnik ◽  
Maciej Niedzielski ◽  
...  
Keyword(s):  
Secale Cereale ◽  
Gene Pool ◽  
Biological Diversity ◽  
Agronomic Traits ◽  
Botanical Garden ◽  
Phenotypic Characterization ◽  
Germplasm Collections ◽  
The World ◽  
Secale Cereale L

Abstract Common rye (Secale cereale L.) is one of the most important cereals in Europe. Nevertheless, its germplasm collections are among the least numerous compared with cereals. There are only about 27,000 Secale accessions in 70 gene banks around the world. Despite extensive research on the molecular characterization of genetic resources, only a fraction of this collection has been described. The main objective of the presented study was to perform genotypic and phenotypic characterization of an obsolete gene pool represented by 100 accessions originated from 28 countries around the world and preserved in the gene bank of the Polish Academy of Sciences Botanical Garden – Center for Biological Diversity Conservation in Powsin. Genetic analysis using simple sequence repeat markers showed that the obsolete gene pool is relatively large. This indicates that different sources of variability were used in breeding programs. However, the genetic variation is in no way related to the place of origin. Despite the great differences in the genetic make-up, the collection showed a broadly common phenotype. This could result in a low level of interest among breeders in the stored germplasm, undervalued as a source of important but not easily observable traits, e.g., high disease resistance, which was found in some accessions.

scholarly journals Morphological Characterization of Bambara Groundnut [Vigna subterranea (L.) Verdc.] Landraces in Kenya

2021 ◽  
Author(s):  
Forrester Odongo ◽  
Maurice E. Oyoo ◽  
Paul K. Kimurto ◽  
Victor W. Wasike
Keyword(s):  
Quantitative Traits ◽  
Block Design ◽  
Leaf Shape ◽  
Seed Morphology ◽  
Bambara Groundnut ◽  
Sub Saharan Africa ◽  
Small Scale ◽  
Growth Stages ◽  
Vigna Subterranea ◽  
Phenotypic Marker

Background: Bambara groundnut [Vigna subterranea (L.) Verdc.], an indigenous drought tolerant crop of African origin is one of most important leguminous crop in Sub-Saharan Africa. Small-scale farmers continue cultivating unimproved landraces over the production areas in Kenya. Bambara exist variously as mixtures of seeds, which contain variable types of seed morphology which need to be agronomically and phenotypically differentiated. The study aimed at characterizing Bambara groundnuts collected in Kenya using morphological markers. Methods: One hundred and five germplasm assembled from four major growing agro-ecologies (Busia, Kakamega, Bungom and Vihiga Counties) and Kenya National Gene bank, were evaluated at the Kenya Agricultural and Livestock Research Organization (KALRO) - Alupe (0.4347° N, 34.2422° E) in a randomized complete block design with three replications in the long and short rains of 2015. Nineteen quantitative traits and seven qualitative traits were observed and measured at different growth stages and during harvesting. Result: Many landraces displayed pointed, round and yellowish pod, with grooved and oval seeds. About 49.4% had round leaves, 21.5% had elliptical leaves, while 55.7% were heterogeneous for leaf shape. Quantitative traits were significant (p≤0.05) except for seed weight, seed number per plant and number of stems. The first four principal components accounted for a total of 73.1% of the variations. germplasm were divided into two distinct clusters. Leaf morphology variations could be used as a reliable phenotypic marker in Bambara breeding.

Comprehensive phenotypic characterization of ABCB4 deficient mice reveals systemic effects of spontaneous biliary fibrosis

2009 ◽  
Vol 47 (01) ◽  
Author(s):  
K Hochrath ◽  
S Hillebrandt ◽  
F Lammert ◽  
B Rathkolb ◽  
H Fuchs ◽  
...  
Keyword(s):  
Phenotypic Characterization ◽  
Systemic Effects ◽  
Deficient Mice

GENETIC DIVERSITY IN BAMBARA GROUNDNUT (Vigna subterranean) AS REVEALED BY MOLECULAR WEIGHTS OF THE SEEDS’ PROTEINS

2018 ◽  
Vol 30 (2) ◽  
pp. 19-28
Author(s):  
A. J. Oludare ◽  
J. I. Kioko ◽  
A. A. Akeem ◽  
A. T. Olumide ◽  
K. R. Justina ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Seed Proteins ◽  
Electrophoretic Analysis ◽  
Protein Characterization ◽  
Bambara Groundnut ◽  
Vigna Subterranea ◽  
Phylogenetic Relatedness ◽  
Molecular Weights ◽  
National Centre ◽  
Standard Marker

Nine accessions of Bambara groundnut (Vigna subterranea (L.) Verdc.,syn. Voandzeia subterranea (L.) Thouars ex DC.)  obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the nine accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions B, C, E, F, H and I have molecular weights not significantly different from one another (P<0.05) while samples A, D and G showed significantly different values (P>0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the nine accessions of Bambara groundnut (Vigna subterraranea (L.) Verdc.syn

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