scholarly journals Genetic variability of Anthoxanthum aristatum Boiss. (Poaceae) at the non-native range limit

2019 ◽  
Vol 67 (1) ◽  
pp. 163-176
Author(s):  
Maria Drapikowska ◽  
Katarzyna Buczkowska ◽  
Zbigniew Celka ◽  
Piotr Szkudlarz ◽  
Bogdan Jackowiak
Keyword(s):  
Genetic Diversity ◽  
Central Europe ◽  
Western Europe ◽  
Morphological Variability ◽  
Native Range ◽  
Range Limit ◽  
High Genetic Diversity ◽  
Arable Field ◽  
Ecological Barriers ◽  
Sandy Grassland

Abstract Anthoxanthum aristatum Boiss. is native to southern and western Europe and North Africa. In Central Europe this species is recognized as invasive. Its ability to colonize various habitats may result from a high level of polymorphic isozyme loci and high morphological variability. The aim of this study was to recognize the degree of genetic diversity of the studied invasive species at its non-native range limit using molecular markers. The examined plant material consisted of 70 samples collected from 7 populations originating from various habitats: arable fields, recently abandoned field and sandy grassland. Ninety-six the Directed Amplification of Minisatellite-region DNA loci and 82 Sequence-Related Amplified Polymorphism loci were detected. The genetic diversity at the species level was high. The majority of genetic variation was present within populations (68% and 66%), while the remaining molecular variation was distributed among populations within and among habitat types. Specimens originating from sandy grassland populations were grouped together in one cluster that was genetically distinct from the arable field and fallow populations. The high genetic diversity populations at the A. aristatum non-native range limit in Central Europe and their marked diversity in arable field and sandy grassland habitats suggest that this species may systematically cross both geographical and ecological barriers.

scholarly journals Characterization of Lebanese Germplasm of Snake Melon (Cucumis melo subsp. melo var. flexuosus) Using Morphological Traits and SSR Markers

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1293
Author(s):  
Joe Merheb ◽  
Magdalena Pawełkowicz ◽  
Ferdinando Branca ◽  
Hanna Bolibok-Brągoszewska ◽  
Agnieszka Skarzyńska ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Skin Color ◽  
Cucumis Melo ◽  
Environmental Changes ◽  
Phenotypic Variability ◽  
Morphological Variability ◽  
Color Pattern ◽  
High Genetic Diversity ◽  
Improvement Programs

Snake melon (Cucumis melo subsp. melo L. var. flexuosus (L.) Naudin) is an ancient and traditional crop in the Mediterranean region. Nevertheless, there has been poor interest in assessing snake melon germplasm where its genetic resources have not been surveyed before despite their potential in adaptation to environmental changes. In this study, we assess the genetic diversity of snake melon landraces collected from different Lebanese regions at both morphological and molecular levels. Morphological characterization using a set of 18 descriptors revealed an important phenotypic variability among the landraces studied. Principle component analysis indicated that fruit hair and its consistency, fruit size, and skin color pattern were good criteria for discriminating among landraces. Based on the scatter plot diagram, landraces of snake melon formed five different groups with one being defined as typical var. flexuosus. Ten simple sequence repeat (SSR) markers were used for the molecular characterization. Fifty-six different alleles were detected, with an average of 5.6 alleles per locus. Polymorphism information content of SSR markers ranged from 0.06 to 0.84 (average 0.38). Cluster analysis based on molecular markers showed high genetic diversity and divided the landraces into five distinct genetic groups, confirming thereby the morphological variability. Findings of this study indicate a significant diversity for the Lebanese snake melon germplasm that must be further conserved and considered in improvement programs of this ancient crop.

Genetic diversity and morphological variability of fifteen Stachys (Lamiaceae) species from Iran using morphological and ISSR molecular markers

Biologia ◽  
2015 ◽  
Vol 70 (4) ◽  
Author(s):  
Navaz Kharazian ◽  
Somayeh Rahimi ◽  
Behrouz Shiran
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Morphological Variability ◽  
Natural Habitats ◽  
High Genetic Diversity ◽  
Morphological Variations ◽  
Gene Variation ◽  
Germplasm Diversity ◽  
Issr Molecular Markers ◽  
Zagros Region

AbstractStachys L. (Lamiaceae) exhibits a great range of variation with cosmopolitan distribution. An area extremely rich in taxa including 34 species has been introduced in Iran. This study documents the genetic diversity and morphological variations of 48 accessions from fifteen Stachys species and two subspecies belonging to six sections. All of the specimens were collected from natural habitats in Zagros region of Iran. In order to evaluate morphological variations, 21 characters were used. Eleven ISSR primers with DNA samples of leaves were applied to assess the genetic variations among species and populations of the Stachys genus. Based on the results, from the eleven primers screened, nine primers produced reproducible bands. The highest percentage of polymorphism included 100% in section Aucheriana. Analysis of molecular variance revealed a high proportion of gene variation (42%) among populations within species. In total sections, the UPGMA clustering dendrogram genetically detected different groups. Based on the morphological analysis, each section was definitely positioned. A high genetic diversity and polymorphism was found among Stachys accessions belonging to each section. Based on the findings, the germplasm diversity was due to the wealth genetic resources in the central Zagros region which are important in plant breeding and gene conservation purposes. In addition, morphological and ISSR molecular markers are useful to display the diversity in Stachys genus.

scholarly journals Unexpected Absence of Population Structure and High Genetic Diversity of the Western Atlantic Hermit Crab Clibanarius antillensis Stimpson, 1859 (Decapoda: Diogenidae) Based on Mitochondrial Markers and Morphological Data

Diversity ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 56
Author(s):  
Keity S. Nishikawa ◽  
Mariana Negri ◽  
Fernando L. Mantelatto
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Hermit Crab ◽  
Mitochondrial Gene ◽  
Morphological Variability ◽  
South Florida ◽  
Morphological Data ◽  
High Genetic Diversity ◽  
Western Atlantic ◽  
Climate Fluctuations

Recent studies on genetic variability have revealed different patterns of genetic structure among populations of marine decapod species with wide geographical distribution. The hermit crab Clibanarius antillensis has a broad distribution along the western Atlantic Ocean, from south Florida (United States) to Santa Catarina (Brazil). This factor, in addition to differences in larval morphology and in adult coloration, makes this species a good model for studies on intraspecific variations. Therefore, we evaluated the molecular and morphological variability of C. antillensis along its distribution in order to check the levels of population structure. The results were based on the morphological analyses of 187 individuals and 38 partial sequences of the mitochondrial gene 16S rRNA and 46 of cytochrome c oxidase subunit I (COI) from specimens whose locations covered the whole species distribution. The molecular analyses did not show any apparent population structure of C. antillensis. This result was corroborated by the morphological analyses since the characters analyzed did not show any pattern of variation. Our results may be explained by a set of factors, such as the dispersive potential of the species and the absence of barriers that could prevent gene flow. In addition, high genetic diversity was observed, mainly for COI, which may be explained by the historical processes of the species, which seem to be in almost constant expansion in the last 700,000 years and experienced no genetic bottleneck. Apparently, this species was little affected by the climate fluctuations of Pleistocene. Additionally, our morphological analyses allowed us to present herein a redescription of the studied species since we noted differences from the characters in the diagnosis.

Broad geographical distribution and high genetic diversity of shrew-borne Seewis hantavirus in Central Europe

Virus Genes ◽  
2012 ◽  
Vol 45 (1) ◽  
pp. 48-55 ◽  
Author(s):  
Mathias Schlegel ◽  
Lukáš Radosa ◽  
Ulrike M. Rosenfeld ◽  
Sabrina Schmidt ◽  
Cornelia Triebenbacher ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Geographical Distribution ◽  
Central Europe ◽  
High Genetic Diversity

scholarly journals Subtype Characterization and Zoonotic Potential of Cryptosporidium felis in Cats in Guangdong and Shanghai, China

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 89
Author(s):  
Jiayu Li ◽  
Fuxian Yang ◽  
Ruobing Liang ◽  
Sheng Guo ◽  
Yaqiong Guo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Genetic Characterization ◽  
Zoonotic Potential ◽  
Common Occurrence ◽  
High Genetic Diversity ◽  
Gp60 Gene ◽  
The Common ◽  
Subtyping Tool

Cryptosporidiumfelis is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of C. felis in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (gp60) gene was employed to identify the subtypes of 30 cat-derived C. felis isolates from Guangdong and Shanghai. Altogether, 20 C. felis isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the gp60 sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of C. felis. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of C. felis.

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