Genetic diversity and morphological variability of fifteen Stachys (Lamiaceae) species from Iran using morphological and ISSR molecular markers

AbstractStachys L. (Lamiaceae) exhibits a great range of variation with cosmopolitan distribution. An area extremely rich in taxa including 34 species has been introduced in Iran. This study documents the genetic diversity and morphological variations of 48 accessions from fifteen Stachys species and two subspecies belonging to six sections. All of the specimens were collected from natural habitats in Zagros region of Iran. In order to evaluate morphological variations, 21 characters were used. Eleven ISSR primers with DNA samples of leaves were applied to assess the genetic variations among species and populations of the Stachys genus. Based on the results, from the eleven primers screened, nine primers produced reproducible bands. The highest percentage of polymorphism included 100% in section Aucheriana. Analysis of molecular variance revealed a high proportion of gene variation (42%) among populations within species. In total sections, the UPGMA clustering dendrogram genetically detected different groups. Based on the morphological analysis, each section was definitely positioned. A high genetic diversity and polymorphism was found among Stachys accessions belonging to each section. Based on the findings, the germplasm diversity was due to the wealth genetic resources in the central Zagros region which are important in plant breeding and gene conservation purposes. In addition, morphological and ISSR molecular markers are useful to display the diversity in Stachys genus.

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ISSR molecular markers for the study of the genetic diversity of Mimosa caesalpiniaefolia Benth.

Idesia (Arica) ◽

10.4067/s0718-34292016000300007 ◽

2016 ◽

Vol 34 (3) ◽

pp. 47-52 ◽

Cited By ~ 6

Author(s):

Fernando dos Santos Araújo ◽

Mauro Vasconcelos Pacheco ◽

Fábio de Almeida Vieira ◽

Cibele dos Santos Ferrari ◽

Francival Cardoso Félix ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Issr Molecular Markers

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Species delimitation and population structure in three Onosma (Boraginaceae) species

Biologia ◽

10.1515/biolog-2015-0151 ◽

2015 ◽

Vol 70 (10) ◽

Author(s):

Masoud Sheidai ◽

Samira Sadeghi ◽

Mahnaz-Arab Ameri ◽

Ahmad-Reza Mehrabeian

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Species Delimitation ◽

Genetic Difference ◽

Morphological Variability ◽

Morphological Diversity ◽

Mantel Test ◽

Geographical Distance ◽

Nj Tree ◽

Issr Molecular Markers

AbstractThe present study aimed to reveal genetic diversity and population structure in three Onosma species i.e., Onosma bulbotrichum DC., O. elwendicum Wettst. and O. sericeum Willd. (Boraginaceae) growing in Iran. This study was performed to check if the ISSR molecular markers could be used in the Onosma species delimitation. We also investigated the infra-specific morphological variability and if we can identify infra-species taxonomic forms. The present study revealed within species genetic and morphological diversity. ISSR data could delimit the studied species as they were separated from each other in NJ tree, maximum parsimony and STRUCTURE analysis. AMOVA and Hickory tests produced significant genetic difference among the studied species. The Mantel test showed no correlation between genetic distance and geographical distance of the populations for all three species. Reticulation analysis and Nm estimation showed gene flow among these populations. We could identify a few ISSR loci that are adaptive. Data obtained can be used in conservation of these medicinal plants in Iran.

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Genetic diversity of Barbary fig (Opuntia ficus-indica) collection in Greece with ISSR molecular markers

Plant Gene ◽

10.1016/j.plgene.2015.04.001 ◽

2015 ◽

Vol 2 ◽

pp. 29-33 ◽

Cited By ~ 7

Author(s):

Ioannis Ganopoulos ◽

Apostolos Kalivas ◽

Nektarios Kavroulakis ◽

Aliki Xanthopoulou ◽

Anna Mastrogianni ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Opuntia Ficus Indica ◽

Issr Molecular Markers

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Genetic diversity of endangered sandalwood (Osyris lanceolata) populations in Kenya using ISSR molecular markers

East African Agricultural and Forestry Journal ◽

10.1080/00128325.2019.1605964 ◽

2019 ◽

Vol 83 (2) ◽

pp. 80-93 ◽

Cited By ~ 2

Author(s):

KP Andiego ◽

OG Dangasuk ◽

DW Odee ◽

FS Omondi ◽

DF Otieno ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Issr Molecular Markers

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Study on Biological Materials with Genetic Diversity of Bupleurum marginatum in Northwest of Hubei Province of China Base on ISSR

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.830.463 ◽

2013 ◽

Vol 830 ◽

pp. 463-468

Author(s):

Shi Ming Du ◽

Gang Wang ◽

Yi Mei Liu ◽

Fang Ye ◽

Rong Jin Sun ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Hubei Province ◽

Germplasm Resources ◽

Closely Related Species ◽

Molecular Features ◽

Exploitation And Utilization ◽

Two Samples ◽

Polymorphic Bands ◽

Issr Molecular Markers

To analyze the genetic diversity of Bupleurum marginatum growing in northwest area of Hubei province of china,and provide the molecular identification method for Bupleurum marginatum and basis for its exploitation and utilization. ISSR Molecular markers were used to analyse the genetic relationship between Bupleurum species in northwest area of Hubei province (NAHP) and the Bupleurum chinense DC.. 12 samples of Bupleurum marginatum were amplified to 81 ISSR bands and there were 42 polymorphic bands, the rate of polymorphic bands was 51.9%. 12 samples of Bupleurum marginatum were clustered into one group, two samples of Bupleurum chinense DC. were clustered into another group. The biodiversity and germplasm resources of Bupleurum marginatum in NAHP is abundant and it is beneficial for the development of Bupleurum marginatum. The method of ISSR marker can significantly distinguish Bupleurum marginatum from its closely related species as the molecular features of the species identification and provide basis for distinguishing different germplasm.

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Morphological and ISSR molecular markers reveal genetic diversity of wild hawthorns (Crataegus songorica K. Koch.) in Xinjiang, China

Journal of Integrative Agriculture ◽

10.1016/s2095-3119(17)61688-5 ◽

2017 ◽

Vol 16 (11) ◽

pp. 2482-2495 ◽

Cited By ~ 4

Author(s):

Fang SHENG ◽

Shu-ying CHEN ◽

Jia TIAN ◽

Peng LI ◽

Xue QIN ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Issr Molecular Markers

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Genetic structure and diversity of the endangered growling grass frog in a rapidly urbanizing region

Royal Society Open Science ◽

10.1098/rsos.140255 ◽

2015 ◽

Vol 2 (8) ◽

pp. 140255 ◽

Cited By ~ 7

Author(s):

Claire C. Keely ◽

Joshua M. Hale ◽

Geoffrey W. Heard ◽

Kirsten M. Parris ◽

Joanna Sumner ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Structure ◽

Dna Sequences ◽

Nuclear Dna ◽

Urban Expansion ◽

Nuclear Gene ◽

Management Approach ◽

Natural Habitats ◽

High Genetic Diversity ◽

Genetic Structure And Diversity

Two pervasive and fundamental impacts of urbanization are the loss and fragmentation of natural habitats. From a genetic perspective, these impacts manifest as reduced genetic diversity and ultimately reduced genetic viability. The growling grass frog ( Litoria raniformis ) is listed as vulnerable to extinction in Australia, and endangered in the state of Victoria. Remaining populations of this species in and around the city of Melbourne are threatened by habitat loss, degradation and fragmentation due to urban expansion. We used mitochondrial DNA (mtDNA) and microsatellites to study the genetic structure and diversity of L. raniformis across Melbourne's urban fringe, and also screened four nuclear gene regions (POMC, RAG-1, Rhod and CRYBA1). The mtDNA and nuclear DNA sequences revealed low levels of genetic diversity throughout remnant populations of L. raniformis . However, one of the four regions studied, Cardinia, exhibited relatively high genetic diversity and several unique haplotypes, suggesting this region should be recognized as a separate Management Unit. We discuss the implications of these results for the conservation of L. raniformis in urbanizing landscapes, particularly the potential risks and benefits of translocation, which remains a contentious management approach for this species.

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Genetic diversity analysis of Rose varieties from Grandiflora group based on ISSR molecular markers

BIO Web of Conferences ◽

10.1051/bioconf/20213800140 ◽

2021 ◽

Vol 38 ◽

pp. 00140

Author(s):

Sophia S. Yudanova ◽

Svetlana A. Plugatar ◽

Zinaida K. Klimenko ◽

Vera K. Zykova ◽

Olena L. Rubtsova ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Diversity Analysis ◽

Morphometric Characters ◽

Climate Conditions ◽

Genetic Diversity Analysis ◽

Statistical Comparison ◽

Separate Branch ◽

Issr Molecular Markers ◽

Continental Climate

Kinship and genetic diversity determination among six rose varieties from the Grandiflora group were carried out using ISSR thechnics. The studied varities were divided into 3 clades: I) ‘Lezginka’, ‘Queen Elizabeth’ and ‘Koralovy surpriz’ varieties; II) ‘Gurzuf’ and ‘Love’ varieties; III) ‘Komsomolsky ogonek’ variety formed a separate branch. This division into clades was confirmed by a statistical comparison of morphometric characters. On the basis of the obtained data, it can be concluded that the analysis using the selected primer group is well-suited for differentiation rose varieties into groups, which makes it possible, for one thing, to determine the genetic distance between varieties, and for another, to use these data in the future by certification of the varieties promising by resistance characters to the continental climate conditions.

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