Broad geographical distribution and high genetic diversity of shrew-borne Seewis hantavirus in Central Europe

Virus Genes ◽  
2012 ◽  
Vol 45 (1) ◽  
pp. 48-55 ◽  
Author(s):  
Mathias Schlegel ◽  
Lukáš Radosa ◽  
Ulrike M. Rosenfeld ◽  
Sabrina Schmidt ◽  
Cornelia Triebenbacher ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Geographical Distribution ◽  
Central Europe ◽  
High Genetic Diversity

Variability and genetic structuring of Sitophilus zeamais according to agroecological zones in Senegal (West of Africa)

2019 ◽  
Vol 9 (2) ◽  
pp. 56-63
Author(s):  
Sarr Ngagne Demba ◽  
Toffène Diome ◽  
Mbacke Sembene
Keyword(s):  
Genetic Diversity ◽  
Geographical Distribution ◽  
Sitophilus Zeamais ◽  
Genetic Structuring ◽  
High Genetic Diversity ◽  
Genetic Studies ◽  
Economic Role ◽  
Its Phylogeny ◽  
Agroecological Zones ◽  
The Individual

Maize plays a socio-economic role in Senegal. However, it is considerably damaged by Sitophilus Zeamais, a beetle of the Curculionidae. Areas likely to favor the survival or extinction of Sitophilus zeamais can be identified because genetic diversity influences the adaptability of the individual. Our study aims to highlight a possible variation and structuring of Sitophilus zeamais according to five agroecological zones (AEZ) in Senegal, namely Nord Bassin Arachidier (NBA), Sud Bassin Arachidier (SBA), Sénégal Oriental Haute Casamance (SOHC), Basse Moyenne Casamance (BMC) and Zone Sylvo-Pastorale (ZSP). The purpose of this study is to reduce crop losses by recommending to farmers peas where the insect is reluctant to survive. So far, genetic studies on this insect have traced its phylogeny and its geographical distribution in Africa in general. Senegal has never been specifically studied. To achieve this goal, 89 individuals were captured in the 5 zones. The exploitation of the cytochrome B gene corresponding to these individuals revealed a high genetic diversity in the NBA, SBA, and SOHC and low in BMC and ZSP, but also a genetic structuring of the insect according to agroecological zones. Thus, the agroecological zones of the NBA, the SBA and the SOHC are favorable to its survival, while those of the BMC and the ZSP limit its expansion.

scholarly journals Genetic diversity of Sterculia quadrifida in Kupang, Indonesia based on RAPD (Random Amplified Polymorphic DNA) markers

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Uslan Uslan ◽  
MADE PHARMAWATI
Keyword(s):  
Genetic Diversity ◽  
Geographical Distribution ◽  
Dna Markers ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Mixed Forest ◽  
Similarity Coefficient ◽  
Community Forest ◽  
High Genetic Diversity ◽  
Ctab Method

Abstract. Uslan, Pharmawat M. 2020. Genetic diversity of Sterculia quadrifida in Kupang, Indonesia based on RAPD (Random Amplified Polymorphic DNA) markers. Biodiversitas 21: 3407-3414. This study aims to determine the genetic diversity of Sterculia quadrifida R.Br. in Kupang based on RAPD markers. Samples of S. quadrifida were collected from the yard and community forest in Kupang City (Sub-districts of Oebobo, Kelapa Lima, Maulafa, and Alak) and mixed forest in the Kupang District (Sub-districts of Kupang Barat, Nekamese, Taebenu, and Fatuleu). DNA was isolated by the CTAB method and amplified by six RAPD primers (OPD-11, OPF-11, UBC-106, UBC-127, UBC-250, and OPB-04). The data were analyzed in the MVSP software using UPGMA method and Nei & Li similarity coefficient. Total of 131 DNA bands ranging from 250-1400 bp was obtained. Populations of S. quadrifida in Kupang were divided into two main clusters and 12 sub-clusters with. The highest genetic diversity was found in Kelapa Lima of 0.1050, while the lowest genetic diversity was found in S. quadrifida population in Fatuleu of 0.0305. The population of S. quadrifida in Kupang has high genetic diversity and also clustered based on their geographical distribution

scholarly journals Genetic variability of Anthoxanthum aristatum Boiss. (Poaceae) at the non-native range limit

2019 ◽  
Vol 67 (1) ◽  
pp. 163-176
Author(s):  
Maria Drapikowska ◽  
Katarzyna Buczkowska ◽  
Zbigniew Celka ◽  
Piotr Szkudlarz ◽  
Bogdan Jackowiak
Keyword(s):  
Genetic Diversity ◽  
Central Europe ◽  
Western Europe ◽  
Morphological Variability ◽  
Native Range ◽  
Range Limit ◽  
High Genetic Diversity ◽  
Arable Field ◽  
Ecological Barriers ◽  
Sandy Grassland

Abstract Anthoxanthum aristatum Boiss. is native to southern and western Europe and North Africa. In Central Europe this species is recognized as invasive. Its ability to colonize various habitats may result from a high level of polymorphic isozyme loci and high morphological variability. The aim of this study was to recognize the degree of genetic diversity of the studied invasive species at its non-native range limit using molecular markers. The examined plant material consisted of 70 samples collected from 7 populations originating from various habitats: arable fields, recently abandoned field and sandy grassland. Ninety-six the Directed Amplification of Minisatellite-region DNA loci and 82 Sequence-Related Amplified Polymorphism loci were detected. The genetic diversity at the species level was high. The majority of genetic variation was present within populations (68% and 66%), while the remaining molecular variation was distributed among populations within and among habitat types. Specimens originating from sandy grassland populations were grouped together in one cluster that was genetically distinct from the arable field and fallow populations. The high genetic diversity populations at the A. aristatum non-native range limit in Central Europe and their marked diversity in arable field and sandy grassland habitats suggest that this species may systematically cross both geographical and ecological barriers.

scholarly journals Subtype Characterization and Zoonotic Potential of Cryptosporidium felis in Cats in Guangdong and Shanghai, China

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 89
Author(s):  
Jiayu Li ◽  
Fuxian Yang ◽  
Ruobing Liang ◽  
Sheng Guo ◽  
Yaqiong Guo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Genetic Characterization ◽  
Zoonotic Potential ◽  
Common Occurrence ◽  
High Genetic Diversity ◽  
Gp60 Gene ◽  
The Common ◽  
Subtyping Tool

Cryptosporidiumfelis is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of C. felis in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (gp60) gene was employed to identify the subtypes of 30 cat-derived C. felis isolates from Guangdong and Shanghai. Altogether, 20 C. felis isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the gp60 sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of C. felis. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of C. felis.

scholarly journals Phylogeography of the Brittle Star Ophiura sarsii Lütken, 1855 (Echinodermata: Ophiuroidea) from the Barents Sea and East Atlantic

Diversity ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 40
Author(s):  
Evgeny Genelt-Yanovskiy ◽  
Yixuan Li ◽  
Ekaterina Stratanenko ◽  
Natalia Zhuravleva ◽  
Natalia Strelkova ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Barents Sea ◽  
Haplotype Diversity ◽  
Brittle Star ◽  
The Arctic ◽  
Coi Gene ◽  
High Genetic Diversity ◽  
Svalbard Archipelago ◽  
Mitochondrial Coi ◽  
The Barents Sea

Ophiura sarsii is a common brittle star species across the Arctic and Sub-Arctic regions of the Atlantic and the Pacific oceans. Ophiurasarsii is among the dominant echinoderms in the Barents Sea. We studied the genetic diversity of O.sarsii by sequencing the 548 bp fragment of the mitochondrial COI gene. Ophiurasarsii demonstrated high genetic diversity in the Barents Sea. Both major Atlantic mtDNA lineages were present in the Barents Sea and were evenly distributed between the northern waters around Svalbard archipelago and the southern part near Murmansk coast of Kola Peninsula. Both regions, and other parts of the O.sarsii range, were characterized by high haplotype diversity with a significant number of private haplotypes being mostly satellites to the two dominant haplotypes, each belonging to a different mtDNA clade. Demographic analyses indicated that the demographic and spatial expansion of O.sarsii in the Barents Sea most plausibly has started in the Bølling–Allerød interstadial during the deglaciation of the western margin of the Barents Sea.

scholarly journals Genetic Diversity of Leuconostoc mesenteroides Isolates from Traditional Montenegrin Brine Cheese

2021 ◽  
Vol 9 (8) ◽  
pp. 1612
Author(s):  
Werner Ruppitsch ◽  
Andjela Nisic ◽  
Patrick Hyden ◽  
Adriana Cabal ◽  
Jasmin Sucher ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Safety Evaluation ◽  
Gene Clusters ◽  
Northern Region ◽  
Genomic Diversity ◽  
High Genetic Diversity ◽  
Pathogenic Factors ◽  
Flavor Development ◽  
Genomic Characterization

In many dairy products, Leuconostoc spp. is a natural part of non-starter lactic acid bacteria (NSLAB) accounting for flavor development. However, data on the genomic diversity of Leuconostoc spp. isolates obtained from cheese are still scarce. The focus of this study was the genomic characterization of Leuconostoc spp. obtained from different traditional Montenegrin brine cheeses with the aim to explore their diversity and provide genetic information as a basis for the selection of strains for future cheese production. In 2019, sixteen Leuconostoc spp. isolates were obtained from white brine cheeses from nine different producers located in three municipalities in the northern region of Montenegro. All isolates were identified as Ln. mesenteroides. Classical multilocus sequence tying (MLST) and core genome (cg) MLST revealed a high diversity of the Montenegrin Ln. mesenteroides cheese isolates. All isolates carried genes of the bacteriocin biosynthetic gene clusters, eight out of 16 strains carried the citCDEFG operon, 14 carried butA, and all 16 isolates carried alsS and ilv, genes involved in forming important aromas and flavor compounds. Safety evaluation indicated that isolates carried no pathogenic factors and no virulence factors. In conclusion, Ln. mesenteroides isolates from Montenegrin traditional cheeses displayed a high genetic diversity and were unrelated to strains deposited in GenBank.

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