A Role of Piperine on Monosodium Urate Crystal-Induced Inflammation—An Experimental Model of Gouty Arthritis

The present study was undertaken to evaluate the therapeutic effects of Huzhang-Guizhi herb pair (HG), firstly included in Hu-Zhang Power documented in Taiping Shenghui Fang, on monosodium urate (MSU) crystals-induced gouty arthritis in rats. We found that pretreatment with HG in rats with gouty arthritis could significantly attenuate the ankle joint swelling, and this beneficial antigout effect might be mediated, at least in part, by inhibiting tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) production in synovial fluid as well as nuclear transcription factor-κB p65 (NF-κB p65) protein expression in synovial tissue. Moreover, metabonomic analysis demonstrated that 5 and 6 potential biomarkers associated with gouty arthritis in plasma and urine, respectively, which were mainly involved in energy metabolism, amino acid metabolism, and gut microbe metabolism, were identified. HG could reverse the pathological process of MSU-induced gouty arthritis through regulating the disturbed metabolic pathways. These results provided important mechanistic insights into the protective effects of HG against MSU-induced gouty arthritis in rats.

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Inhibition of Monosodium Urate Crystal-Induced Inflammation by Withaferin A

Journal of Pharmacy & Pharmaceutical Sciences ◽

10.18433/j35k58 ◽

2009 ◽

Vol 11 (4) ◽

pp. 46 ◽

Cited By ~ 1

Author(s):

Mahaboobkhan Rasool ◽

Sonal Chandal ◽

Evan Prince Sabina

Keyword(s):

Lipid Peroxidation ◽

Lactate Dehydrogenase ◽

Lysosomal Enzymes ◽

Inflammatory Mediator ◽

Gouty Arthritis ◽

Withaferin A ◽

Polymorphonuclear Leucocytes ◽

Monosodium Urate ◽

Urate Crystal ◽

Anti Inflammatory

ABSTRACT Purpose. Gouty arthritis is a characteristically intense acute inflammatory reaction resulting from the formation of sodium urate crystals in the joint cavity. In the present study, the effect of withaferin A, a steroidal lactone was investigated on monosodium urate crystal-induced inflammation in mice; an experimental model for gouty arthritis and compared it with that of the non-steroidal anti-inflammatory drug, indomethacin. Methods. Paw volume and levels/activities of lysosomal enzymes, lipid peroxidation, anti-oxidant status and inflammatory mediator TNF-α were determined in control and monosodium urate crystal-induced mice. The levels of β-glucuronidase and lactate dehydrogenase were also measured in monosodium urate crystal-incubated polymorphonuclear leucocytes (PMNL). Results. Paw volume, the levels of lysosomal enzymes, lipid peroxidation, and inflammatory mediator tumour necrosis factor-α were found to be increased significantly and the activities of antioxidant status were in turn decreased in monosodium urate crystal-induced mice; however these changes were reverted back to near normal levels in withaferin A (30 mg/kg/b.wt, i.p.) treated monosodium urate crystal-induced mice. In addition, β-glucuronidase and lactate dehydrogenase level were reduced in withaferin A (100μg/ml) treated monosodium urate crystal-incubated polymorphonuclear leucocytes. Conclusion. The present findings clearly indicated that withaferin A exerted a strong anti-inflammatory effect against gouty arthritis.

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