Anti-inflammatory effects of traditional mixed extract of medicinal herbs (MEMH) on monosodium urate crystal-induced gouty arthritis

ABSTRACT Purpose. Gouty arthritis is a characteristically intense acute inflammatory reaction resulting from the formation of sodium urate crystals in the joint cavity. In the present study, the effect of withaferin A, a steroidal lactone was investigated on monosodium urate crystal-induced inflammation in mice; an experimental model for gouty arthritis and compared it with that of the non-steroidal anti-inflammatory drug, indomethacin. Methods. Paw volume and levels/activities of lysosomal enzymes, lipid peroxidation, anti-oxidant status and inflammatory mediator TNF-α were determined in control and monosodium urate crystal-induced mice. The levels of β-glucuronidase and lactate dehydrogenase were also measured in monosodium urate crystal-incubated polymorphonuclear leucocytes (PMNL). Results. Paw volume, the levels of lysosomal enzymes, lipid peroxidation, and inflammatory mediator tumour necrosis factor-α were found to be increased significantly and the activities of antioxidant status were in turn decreased in monosodium urate crystal-induced mice; however these changes were reverted back to near normal levels in withaferin A (30 mg/kg/b.wt, i.p.) treated monosodium urate crystal-induced mice. In addition, β-glucuronidase and lactate dehydrogenase level were reduced in withaferin A (100μg/ml) treated monosodium urate crystal-incubated polymorphonuclear leucocytes. Conclusion. The present findings clearly indicated that withaferin A exerted a strong anti-inflammatory effect against gouty arthritis.

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LC-MS Analysis of Serum for the Metabolomic Investigation of the Effects of Pulchinenoside b4 Administration in Monosodium Urate Crystal-Induced Gouty Arthritis Rat Model

Molecules ◽

10.3390/molecules24173161 ◽

2019 ◽

Vol 24 (17) ◽

pp. 3161

Author(s):

Shang Lyu ◽

Ruowen Ding ◽

Peng Liu ◽

Hui OuYang ◽

Yulin Feng ◽

...

Keyword(s):

Rat Model ◽

Clinical Symptoms ◽

Biological Activities ◽

Gouty Arthritis ◽

Joint Inflammation ◽

Ultra Performance Liquid Chromatography ◽

Monosodium Urate ◽

Wide Range ◽

Urate Crystal ◽

Anti Inflammatory

Gouty arthritis (GA) is commonly caused by deposition of monosodium urate (MSU) crystals within the joint capsule, bursa, cartilage, bone, or other periarticular tissues after chronic hyperuricemia. Clinically, GA is characterized by acute episodes of joint inflammation, which is most frequently encountered in the major joints, and also has a significant impact on quality of life. Pulchinenoside b4(P-b4) has a wide range of biological activities, including antitumor, anti-inflammatory, antiviral and immunomodulatory activities. Currently, the anti-GA activity and metabolomic profiles after being treated by P-b4 have not been reported. In this paper, for the first time, we have performed a non-targeted metabolomics analysis of serum obtained from an MSU crystal-induced GA rat model intervened by P-b4, using ultra-performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry. In this study, the main pharmacodynamics of different dosing methods and dosages of P-b4 was firstly investigated. Results have shown that P-b4 possesses high anti-inflammatory activity. These results demonstrated changes in serum metabolites with 32 potential biomarkers. Arachidonic acid, sphingolipid, and glycerophospholipid metabolism are considered to be the most relevant metabolic pathway with P-b4 treatment effect in this study. Moreover, the changes of metabolites and the self-extinction of model effects within 24 h reveals important information for GA diagnostic criteria: The regression of clinical symptoms or the decline of some biochemical indicators cannot be regarded as the end point of GA treatment. Furthermore, our research group plans to conduct further metabolomics research on the clinical course of GA.

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Tripterine ameliorates monosodium urate crystal-induced gouty arthritis by altering macrophage polarization via the miR-449a/NLRP3 axis

Inflammation Research ◽

10.1007/s00011-021-01439-0 ◽

2021 ◽

Vol 70 (3) ◽

pp. 323-341

Author(s):

Yu Wang

Keyword(s):

Macrophage Polarization ◽

Gouty Arthritis ◽

Monosodium Urate ◽

Urate Crystal

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Ozone Therapy Alleviates Monosodium Urate Induced Acute Gouty Arthritis in Rats Through Inhibition of NLRP3 Inflammasome

Current Drug Therapy ◽

10.2174/1574885516666210719092523 ◽

2021 ◽

Vol 16 ◽

Author(s):

Doaa M. Abdullah ◽

Soad L. Kabil

Keyword(s):

Gouty Arthritis ◽

Antioxidant Defense System ◽

Albino Rats ◽

Monosodium Urate ◽

Ozone Therapy ◽

Reference Drug ◽

Acute Gouty Arthritis ◽

Beneficial Effects ◽

Anti Inflammatory ◽

Domain 3

Background: Gout is a metabolic disease strictly related to hyperuricemia. The associated intense inflammation and pain are triggered by the deposited monosodium urate crystals (MSU) in joints. The principal therapeutic strategies of gout involve the control of hyperuricemia and anti-inflammatory medications. Objectives: This study aimed to investigate the possible beneficial effects of ozone therapy, a well-known antioxidant, and an immunomodulation, on gouty arthritis and the underlying mechanisms. Methods : Acute gouty arthritis was induced in male albino rats via MSU crystals intra-articular injection in the ankle joint. The gouty arthritic rats received pre-treatment with ozone, colchicine (as a reference drug), or combination. Results : The obtained results of ozone therapy showed obvious reduction in the degree of ankle edematous swelling, pro-inflammatory cytokines, lipid peroxidation, the nucleotide binding oligomerization domain like receptor containing pyrin domain 3 (NLRP3), procaspase-1, caspase-1, interleukin-1β synovial tissue levels with enhancement of antioxidant defense system. Additionally, ozone therapy significantly attenuated the histological derangements in gouty arthritic rats. Conclusion : This study suggests that ozone is able to treat gouty arthritis and reducing synovial injury through an anti-inflammatory effect as well as antioxidant activity.

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Trikatu, a herbal compound that suppresses monosodium urate crystal-induced inflammation in rats, an experimental model for acute gouty arthritis

Cell Biochemistry and Function ◽

10.1002/cbf.2979 ◽

2013 ◽

Vol 32 (1) ◽

pp. 106-114 ◽

Cited By ~ 13

Author(s):

Vachana Murunikkara ◽

MahaboobKhan Rasool

Keyword(s):

Experimental Model ◽

Gouty Arthritis ◽

Monosodium Urate ◽

Acute Gouty Arthritis ◽

Herbal Compound ◽

Urate Crystal

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Anti-inflammatory effect of Sustained release granules of Aceclofenac in Gouty Arthritis Rat

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00996 ◽

2021 ◽

pp. 5729-5732

Author(s):

Rajiv Kumar ◽

Parminder Nain ◽

Jaspreet Kaur ◽

Ravi Kumar Dhawan

Keyword(s):

Sustained Release ◽

Therapeutic Effect ◽

Slow Release ◽

Standard Dose ◽

Gouty Arthritis ◽

Control Group ◽

Monosodium Urate ◽

Sustained Release Formulation ◽

Anti Inflammatory ◽

Inflammatory Effect

Gout is most common painful clinical syndrome occurs due to hyperuricaemia (high serum uric acid level) and deposition of monosodium urate crystals in joints. In the current experimental study, the anti-inflammatory effect of sustained release granules of aceclofenac, was investigated on monosodium urate crystal-induced inflammation in rat. Monosodium urate is injected appropriately at a dose of 3 mg/kg body weight of a rat on right ankle to induce inflammation in joint like gouty arthritis. The percentage of joint swelling in positive control group was increased significantly (p<0.5) when compared with normal group after 1 hr, 12 hr and 24 hr with a single injection of MSU. Therapeutic effect of sustained released granules (OD) is similar to conventional released granules (BD) and possesses an anti-inflammatory effect, which could provide relief in gouty arthritis after administration of sustained release formulation of aceclofenac once in a day only. The current study clearly indicated that slow release granules of aceclofenac exerted a strong anti-inflammatory effect against gouty arthritis at standard dose once daily. It concluded that, when aceclofenac tablet is formulated with slow release granules it decreases the dosing frequency with same therapeutic effect.

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Therapeutic Effects of Chinese Medicine Herb Pair, Huzhang and Guizhi, on Monosodium Urate Crystal-Induced Gouty Arthritis in Rats Revealed by Anti-Inflammatory Assessments and NMR-Based Metabonomics

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/9398435 ◽

2016 ◽

Vol 2016 ◽

pp. 1-12 ◽

Cited By ~ 6

Author(s):

Bin Han ◽

Huizhu Huang ◽

Zhong Li ◽

Mengjuan Gong ◽

Wan Shi ◽

...

Keyword(s):

Interleukin 1 ◽

Gouty Arthritis ◽

Pathological Process ◽

Therapeutic Effects ◽

Protective Effects ◽

Monosodium Urate ◽

Necrosis Factor Alpha ◽

Gut Microbe ◽

Urate Crystal ◽

Factor Alpha

The present study was undertaken to evaluate the therapeutic effects of Huzhang-Guizhi herb pair (HG), firstly included in Hu-Zhang Power documented in Taiping Shenghui Fang, on monosodium urate (MSU) crystals-induced gouty arthritis in rats. We found that pretreatment with HG in rats with gouty arthritis could significantly attenuate the ankle joint swelling, and this beneficial antigout effect might be mediated, at least in part, by inhibiting tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) production in synovial fluid as well as nuclear transcription factor-κB p65 (NF-κB p65) protein expression in synovial tissue. Moreover, metabonomic analysis demonstrated that 5 and 6 potential biomarkers associated with gouty arthritis in plasma and urine, respectively, which were mainly involved in energy metabolism, amino acid metabolism, and gut microbe metabolism, were identified. HG could reverse the pathological process of MSU-induced gouty arthritis through regulating the disturbed metabolic pathways. These results provided important mechanistic insights into the protective effects of HG against MSU-induced gouty arthritis in rats.

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