Tripterine ameliorates monosodium urate crystal-induced gouty arthritis by altering macrophage polarization via the miR-449a/NLRP3 axis

The present study was undertaken to evaluate the therapeutic effects of Huzhang-Guizhi herb pair (HG), firstly included in Hu-Zhang Power documented in Taiping Shenghui Fang, on monosodium urate (MSU) crystals-induced gouty arthritis in rats. We found that pretreatment with HG in rats with gouty arthritis could significantly attenuate the ankle joint swelling, and this beneficial antigout effect might be mediated, at least in part, by inhibiting tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) production in synovial fluid as well as nuclear transcription factor-κB p65 (NF-κB p65) protein expression in synovial tissue. Moreover, metabonomic analysis demonstrated that 5 and 6 potential biomarkers associated with gouty arthritis in plasma and urine, respectively, which were mainly involved in energy metabolism, amino acid metabolism, and gut microbe metabolism, were identified. HG could reverse the pathological process of MSU-induced gouty arthritis through regulating the disturbed metabolic pathways. These results provided important mechanistic insights into the protective effects of HG against MSU-induced gouty arthritis in rats.

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Inhibition of Monosodium Urate Crystal-Induced Inflammation by Withaferin A

Journal of Pharmacy & Pharmaceutical Sciences ◽

10.18433/j35k58 ◽

2009 ◽

Vol 11 (4) ◽

pp. 46 ◽

Cited By ~ 1

Author(s):

Mahaboobkhan Rasool ◽

Sonal Chandal ◽

Evan Prince Sabina

Keyword(s):

Lipid Peroxidation ◽

Lactate Dehydrogenase ◽

Lysosomal Enzymes ◽

Inflammatory Mediator ◽

Gouty Arthritis ◽

Withaferin A ◽

Polymorphonuclear Leucocytes ◽

Monosodium Urate ◽

Urate Crystal ◽

Anti Inflammatory

ABSTRACT Purpose. Gouty arthritis is a characteristically intense acute inflammatory reaction resulting from the formation of sodium urate crystals in the joint cavity. In the present study, the effect of withaferin A, a steroidal lactone was investigated on monosodium urate crystal-induced inflammation in mice; an experimental model for gouty arthritis and compared it with that of the non-steroidal anti-inflammatory drug, indomethacin. Methods. Paw volume and levels/activities of lysosomal enzymes, lipid peroxidation, anti-oxidant status and inflammatory mediator TNF-α were determined in control and monosodium urate crystal-induced mice. The levels of β-glucuronidase and lactate dehydrogenase were also measured in monosodium urate crystal-incubated polymorphonuclear leucocytes (PMNL). Results. Paw volume, the levels of lysosomal enzymes, lipid peroxidation, and inflammatory mediator tumour necrosis factor-α were found to be increased significantly and the activities of antioxidant status were in turn decreased in monosodium urate crystal-induced mice; however these changes were reverted back to near normal levels in withaferin A (30 mg/kg/b.wt, i.p.) treated monosodium urate crystal-induced mice. In addition, β-glucuronidase and lactate dehydrogenase level were reduced in withaferin A (100μg/ml) treated monosodium urate crystal-incubated polymorphonuclear leucocytes. Conclusion. The present findings clearly indicated that withaferin A exerted a strong anti-inflammatory effect against gouty arthritis.

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Morin, a dietary bioflavonol suppresses monosodium urate crystal-induced inflammation in an animal model of acute gouty arthritis with reference to NLRP3 inflammasome, hypo-xanthine phospho-ribosyl transferase, and inflammatory mediators

European Journal of Pharmacology ◽

10.1016/j.ejphar.2016.06.005 ◽

2016 ◽

Vol 786 ◽

pp. 116-127 ◽

Cited By ~ 32

Author(s):

Chitra Dhanasekar ◽

Mahaboobkhan Rasool

Keyword(s):

Animal Model ◽

Inflammatory Mediators ◽

Nlrp3 Inflammasome ◽

Gouty Arthritis ◽

Monosodium Urate ◽

Acute Gouty Arthritis ◽

Urate Crystal

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Studies on the Anti-Gouty Arthritis and Anti-hyperuricemia Properties of Astilbin in Animal Models

Open Chemistry ◽

10.1515/chem-2020-0023 ◽

2020 ◽

Vol 18 (1) ◽

pp. 207-214

Author(s):

Han Yan ◽

Lanzhou Li ◽

Xue Jiang ◽

Shaopeng Li ◽

Zecheng Chang ◽

...

Keyword(s):

Inflammatory Cell ◽

Gouty Arthritis ◽

Monosodium Urate ◽

Necrosis Factor Alpha ◽

Monocyte Chemoattractant Protein 1 ◽

Acute Gouty Arthritis ◽

Ankle Joints ◽

Urate Crystal ◽

Factor Alpha ◽

Necrosis Factor

AbstractThe purpose of this study was to investigate potential anti-gouty effect of astilbin (AS) and its possible mechanisms. In mice with hyperuricemia induced by potassium oxonate (OXO) and yeast extract powder (YEP), AS and febuxostat (FB) reduced the serum uric acid (UA) and xanthine oxidase (XO). Moreover, AS and FB reduced the levels of reactive oxygen species and increased the content of superoxide dismutase (SOD), glutathione peroxidase and catalase present in the serum. In acute gouty arthritis rats induced by intraarticular monosodium urate crystal injection, AS and Colchicine (COL) alleviated the ankle joints swelling, and reduced the inflammatory cell infiltration. AS also reduced the levels of interleukin 1β, interleukin 6, tumor necrosis factor alpha and monocyte chemoattractant protein 1 in liver. The present study first confirmed the anti-gouty effect of AS in mice with hyperuricemia and rats with acute gouty arthritis, which provides the experimental evidence for further evaluation of AS as a candidate for gout treatment.

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An Improved Acute Gouty Arthritis Rat Model and Therapeutic Effect of Rhizoma Dioscoreae Nipponicae on Acute Gouty Arthritis Based on the Protein-Chip Methods

The American Journal of Chinese Medicine ◽

10.1142/s0192415x12500103 ◽

2012 ◽

Vol 40 (01) ◽

pp. 121-134 ◽

Cited By ~ 6

Author(s):

Li Yao ◽

Wanru Dong ◽

Fang Lu ◽

Shumin Liu

Keyword(s):

Previous Experiment ◽

Gouty Arthritis ◽

Pathological Process ◽

Antibody Array ◽

Monosodium Urate ◽

Blood Samples ◽

Acute Gouty Arthritis ◽

Protein Quantitation ◽

Urate Crystal ◽

Set Up

Rhizoma Dioscoreae Nipponicae (RDN) is an herbal medicine. In the theories of Traditional Chinese Medicine (TCM), the function of RDN is to expel wind and remove dampness. Inflammatory mechanisms play an important role in the pathological process and prognosis of acute gouty arthritis (AGA). The aim of this study was to determine the specially expressed proteins through testing the proteins of the synovium in rats with AGA. The animal model of AGA was set up by Monosodium urate crystal (MSU) combined with hypoxanthine (HX), which was ameliorated in our previous experiment. Blood samples for measurement of serum uric acid were collected prior to sacrifice. Outcomes were assessed (two days after injection) by histological stain and protein quantitation. Three chips of RayBioⓇHuman Label-based Antibody Array I were applied to detect 90 proteins in the synovium tissue of AGA rats. 14 differently expressed proteins were found in the synovium of AGA rats, and nine of them were first found in this model. There were seven up-regulated and seven down-regulated proteins, both TRAIL and Neuropilin-2 could be identified as key contributors to the pathomechanism of AGA.

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A Role of Piperine on Monosodium Urate Crystal-Induced Inflammation—An Experimental Model of Gouty Arthritis

Inflammation ◽

10.1007/s10753-010-9222-3 ◽

2010 ◽

Vol 34 (3) ◽

pp. 184-192 ◽

Cited By ~ 43

Author(s):

Evan Prince Sabina ◽

Shruthi Nagar ◽

Mahaboobkhan Rasool

Keyword(s):

Experimental Model ◽

Gouty Arthritis ◽

Monosodium Urate ◽

Urate Crystal

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Galectin-9 Regulates Monosodium Urate Crystal-Induced Gouty Inflammation Through the Modulation of Treg/Th17 Ratio

Frontiers in Immunology ◽

10.3389/fimmu.2021.762016 ◽

2021 ◽

Vol 12 ◽

Author(s):

Adel Abo Mansour ◽

Federica Raucci ◽

Anella Saviano ◽

Samantha Tull ◽

Francesco Maione ◽

...

Keyword(s):

Inflammatory Response ◽

Molecular Mechanisms ◽

Gouty Arthritis ◽

Joint Inflammation ◽

Monosodium Urate ◽

T Helper 17 ◽

Inflammatory Monocytes ◽

Urate Crystal

Gout is caused by depositing monosodium urate (MSU) crystals within the articular area. The infiltration of neutrophils and monocytes drives the initial inflammatory response followed by lymphocytes. Interestingly, emerging evidence supports the view that in situ imbalance of T helper 17 cells (Th17)/regulatory T cells (Treg) impacts the subsequent damage to target tissues. Galectin-9 (Gal-9) is a modulator of innate and adaptive immunity with both pro- and anti-inflammatory functions, dependent upon its expression and cellular location. However, the specific cellular and molecular mechanisms by which Gal-9 modulates the inflammatory response in the onset and progression of gouty arthritis has yet to be elucidated. In this study, we sought to comprehensively characterise the functional role of exogenous Gal-9 in an in vivo model of MSU crystal-induced gouty inflammation by monitoring in situ neutrophils, monocytes and Th17/Treg recruited phenotypes and related cyto-chemokines profile. Treatment with Gal-9 revealed a dose-dependent reduction in joint inflammation scores, knee joint oedema and expression of different pro-inflammatory cyto-chemokines. Furthermore, flow cytometry analysis highlighted a significant modulation of infiltrating inflammatory monocytes (CD11b+/CD115+/LY6-Chi) and Th17 (CD4+/IL-17+)/Treg (CD4+/CD25+/FOXP-3+) cells following Gal-9 treatment. Collectively the results presented in this study indicate that the administration of Gal-9 could provide a new therapeutic strategy for preventing tissue damage in gouty arthritic inflammation and, possibly, in other inflammatory-based diseases.

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