Elevated Endomyocardial Biopsy Macrophage-Related Markers in Intractable Myocardial Diseases

Inflammation ◽  
2015 ◽  
Vol 38 (6) ◽  
pp. 2288-2299 ◽  
Author(s):  
Yuka Hayashi ◽  
Haruo Hanawa ◽  
Shuang Jiao ◽  
Go Hasegawa ◽  
Yukako Ohno ◽  
...  
Keyword(s):  
Endomyocardial Biopsy ◽  
Myocardial Diseases

scholarly journals Endomyocardial Biopsy in Pediatric Myocarditis and Dilated Cardiomyopathy: A Tool in Search for a Role

2022 ◽  
Vol 9 (1) ◽  
pp. 24
Author(s):  
Mara Pilati ◽  
Micol Rebonato ◽  
Roberto Formigari ◽  
Gianfranco Butera
Keyword(s):  
Endomyocardial Biopsy ◽  
Ventricular Dysfunction ◽  
Diagnostic Technique ◽  
Left Ventricular ◽  
Severe Left Ventricular Dysfunction ◽  
Myocardial Diseases ◽  
Pediatric Heart Failure ◽  
Diagnostic Work ◽  
Work Up

Endomyocardial biopsy (EMB) is a well-known diagnostic tool for the investigation and treatment of myocardial diseases and remains the gold standard for the diagnosis of myocarditis. Due to its invasiveness, with a complication rate ranging from 1 to 15%, its role in the diagnostic work-up of pediatric heart failure is not well established. The aim of this review is to define the role of EMB as diagnostic technique in the work up of children presenting with severe left ventricular dysfunction with the support of our center experience.

Clinical and pathologic analysis of myocardial diseases employing endomyocardial biopsy

1985 ◽  
Vol 1 (S1) ◽  
pp. 68-70
Author(s):  
Wang Pu ◽  
Li Chong-Ren ◽  
Li Xiang-Zhong ◽  
Yu Wei-Han
Keyword(s):  
Endomyocardial Biopsy ◽  
Myocardial Diseases ◽  
Pathologic Analysis

scholarly journals Rationale for endomyocardial biopsy in the diagnosis of heart disease in children and adults

2021 ◽  
Vol 26 (11) ◽  
pp. 4710
Author(s):  
L. B. Mitrofanova ◽  
B. E. Galkovsky ◽  
I. A. Danilova ◽  
D. S. Lebedev
Keyword(s):  
Heart Transplantation ◽  
Endomyocardial Biopsy ◽  
Immunohistochemical Study ◽  
Immunohistochemical Analysis ◽  
Arrhythmogenic Right Ventricular Dysplasia ◽  
Viral Envelope ◽  
Viral Envelope Protein ◽  
Exact Test ◽  
Myocardial Diseases ◽  
Wide Range

Endomyocardial biopsy (EMB) is the method of choice for diagnosing a wide range of myocardial diseases.Aim. To assess the rationale for diagnostic EMB in children and adults.Material and methods. Morphological and statistical analysis of 2803 diagnostic EMBs in adults (n=811) and children (n=83), including those in heart transplantation (n=1909), was carried out.Results. In 231 (28%) cases, adults were diagnosed with myocarditis, of which in 6 patients — granulomatous, in 5 — eosinophilic and in 6 — lymphocytic-macrophage myocarditis after coronavirus infection. In children, myocarditis was found in 22 cases (27%). Arrhythmogenic right ventricular dysplasia took the second place in detection rate in children and adults. Immunohistochemical study revealed viral envelope protein 1 (VP1) antigen of enteroviruses in one third of myocarditis cases, and in half — other cardiotropic viruses. Dotted dystrophin expression was observed in myocarditis. A correlation was established between the perforin expression and myocarditis presence (Pearson χ2=27,8; Fisher's exact test=27,3; p=0,01).Conclusion. Analysis of diagnostic EMB results confirmed its rationale in adults and children not only for heart transplantation, but also for identifying cardiac pathology, including for myocarditis diagnosis. It has been shown that immunohistochemical study with antiviral antibodies can be considered as an alternative method for detecting viral infection. An immunohistochemical analysis for perforin and dystrophin can be recommended as additional morphological markers of myocarditis.

Myocarditis: clinical presentation

ESC CardioMed ◽  
2018 ◽  
pp. 1515-1521
Author(s):  
Alida L. P Caforio ◽  
Renzo Marcolongo ◽  
Sabino Iliceto
Keyword(s):  
Heart Failure ◽  
Endomyocardial Biopsy ◽  
Clinical Presentation ◽  
Task Force ◽  
Signs And Symptoms ◽  
Myocardial Inflammation ◽  
Myocardial Diseases ◽  
Artery Disease ◽  
European Society Of Cardiology ◽  
New Criteria

Myocarditis has no typical clinical presentation, and may mimic a variety of non-inflammatory myocardial diseases. Cardiac signs and symptoms lack specificity, depending on the degree of myocardial inflammation and ventricular dysfunction, and may be subtle; thus the disease may be unrecognized. Myocarditis may resolve spontaneously, recur, or become chronic leading to dilated cardiomyopathy, sudden or heart failure-related death, or heart transplantation. In 2013, a European Society of Cardiology (ESC) Myocarditis Task Force proposed new criteria for clinically suspected myocarditis, using the combination of a plausible clinical presentation and diagnostic criteria. Importantly, diagnosis requires exclusion of non-inflammatory disease (e.g. coronary artery disease) that could explain specific presentations. The ESC 2013 Task Force also recommended a systematic consideration of endomyocardial biopsy in clinically suspected myocarditis, provided that endomyocardial biopsy includes standard histology, immunohistochemistry, and molecular analysis for infectious agents.

scholarly journals Usefulness and limitation of endomyocardial biopsy for the diagnosis of myocardial diseases in children

1995 ◽  
Vol 58 (SupplementIV) ◽  
pp. 1206-1209
Author(s):  
Tetsuro Kamiya ◽  
Hiroki Kajino ◽  
Yasuo Ono ◽  
Chikao / Yutani ◽  
Makoto Takamiya ◽  
...  
Keyword(s):  
Endomyocardial Biopsy ◽  
Myocardial Diseases

Myocarditis: clinical presentation

ESC CardioMed ◽  
2018 ◽  
pp. 1515-1521
Author(s):  
Alida L. P Caforio ◽  
Renzo Marcolongo ◽  
Sabino Iliceto
Keyword(s):  
Heart Failure ◽  
Endomyocardial Biopsy ◽  
Clinical Presentation ◽  
Task Force ◽  
Signs And Symptoms ◽  
Myocardial Inflammation ◽  
Myocardial Diseases ◽  
Artery Disease ◽  
European Society Of Cardiology ◽  
New Criteria

Myocarditis has no typical clinical presentation, and may mimic a variety of non-inflammatory myocardial diseases. Cardiac signs and symptoms lack specificity, depending on the degree of myocardial inflammation and ventricular dysfunction, and may be subtle; thus the disease may be unrecognized. Myocarditis may resolve spontaneously, recur, or become chronic leading to dilated cardiomyopathy, sudden or heart failure-related death, or heart transplantation. In 2013, a European Society of Cardiology (ESC) Myocarditis Task Force proposed new criteria for clinically suspected myocarditis, using the combination of a plausible clinical presentation and diagnostic criteria. Importantly, diagnosis requires exclusion of non-inflammatory disease (e.g. coronary artery disease) that could explain specific presentations. The ESC 2013 Task Force also recommended a systematic consideration of endomyocardial biopsy in clinically suspected myocarditis, provided that endomyocardial biopsy includes standard histology, immunohistochemistry, and molecular analysis for infectious agents.

scholarly journals Immunofluorescent Localization of Plakoglobin Is Altered in Endomyocardial Biopsy Samples from Dogs with Clinically Relevant Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC)

2021 ◽  
Vol 8 (11) ◽  
pp. 248
Author(s):  
Eva M. Oxford ◽  
Romain Pariaut ◽  
Massimiliano Tursi ◽  
Philip R. Fox ◽  
Roberto A. Santilli
Keyword(s):  
Right Ventricular ◽  
Cardiac Disease ◽  
Endomyocardial Biopsy ◽  
Arrhythmogenic Right Ventricular Cardiomyopathy ◽  
Clinical Status ◽  
Clinical Signs ◽  
Ventricular Cardiomyopathy ◽  
Immunofluorescent Localization ◽  
Myocardial Diseases ◽  
The Right

Diagnosing the early stages of canine Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) is complicated by day-to-day arrhythmia variability, and absence of reliable, transthoracic echocardiographic features. Definitive diagnosis requires histopathologic identification of transmural fibrofatty replacement of the right ventricle. Reduction of immunofluorescent signal for plakoglobin (PG) at the intercalated disc (ID) is reported in ARVC-affected humans and boxers. Our objective was to determine whether reduced immunofluorescent signal for PG in endomyocardial biopsy samples (EMBs) correspond with a histopathologic diagnosis of ARVC. Here, 49 dogs were evaluated: 43 with advanced cardiac disease and 6 non-clinical boxers with mild to moderate ventricular arrhythmia (VA) burden. EMBs were obtained from all dogs; samples were prepared with antibodies recognizing cadherin (PC) and PG and evaluated with confocal microscopy. Investigators were blinded to breed and clinical status. ARVC was histopathologically diagnosed in 8 out of 49 dogs. Of these, three out of eight had clinical signs consistent with ARVC (two boxers, one English bulldog) and reduced PG signal at ID; five out of eight were non-clinical boxers with moderate VA and no reduction in PG. A total of 41 out of 49 dogs were histopathologically diagnosed with non-ARVC cardiac disease; 1 out of 41 showed reduction of PG at ID, while 40 out of 41 had no PG reduction. These results suggest that EMB PG signal is reduced in dogs with advanced ARVC, but not in the occult phase of the disease. Additionally, presence of PG at ID supports a diagnosis of non-ARVC cardiac disease in dogs with clinical signs. These results may offer an additional test that helps differentiate advanced ARVC from other myocardial diseases.

Studies on Ultrastructure and Cytochemistry of Right Ventricular Endomyocardial Biopsy

1990 ◽  
Vol 48 (3) ◽  
pp. 256-257
Author(s):  
Xia Mingyu ◽  
Ma Wengshu ◽  
Wu Xiangh ◽  
Chen Dong
Keyword(s):  
Light Microscopy ◽  
Right Ventricular ◽  
Dilated Cardiomyopathy ◽  
Endomyocardial Biopsy ◽  
Heart Diseases ◽  
Ultrastructural Localization ◽  
Controlled Study ◽  
Myocardial Biopsy ◽  
Morphological Assessment ◽  
Rheumatic Heart

This paper describes morphological and cytochemistry changes of endomyocardial biopsy in 94 patients. The samples of myoicardium were taken from 32 patients with dilated cardiomyopathy, and sdudied with light and electron microscop. The cytochemical studies in some of these patients were performed at histological and ultrastructure level. This paper also reported the result of myocardial biopsy in 33 patients with serious dysrythmia.The result of this controlled study indicates that morphological assessment in both cardiomyopathy and congenital or rheumatic heart diseases showed no special changes. In patients of dilated cardiomyopathy, the decreased activity of myosin ATPase was secondary to cardial failure. The change of succinate dehydrogenase (SDHase) was not significant with light microscopy. But ultrastructural localization of SDHase activity is valuable. Its activity was found to be localized in endomembrane and ridge of the mitochondria, the activity of this enzyme was decrease, normal, or increase. SDHase activity was more intense in cardial myocytes well-functioning, or ultrastructurally well preserved hearts.

Myocarditis and Endomyocardial Biopsy

1984 ◽  
Vol 2 (4) ◽  
pp. 647-656 ◽  
Author(s):  
William D. Edwards
Keyword(s):  
Endomyocardial Biopsy
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