The state of the alveolar bone tissue depending on the vitality of the tooth pulp according to the saliva indicators of patients with moderate periodontitis

Aim. To evaluate the results of surgical treatment of patients with moderate chronic periodontitis with different dental pulp status by determining sRANKL, OPG and FGF-2 in mixed saliva and dynamic densitotomometry data.Materials and methods. Surgical treatment of 24 patients with moderate chronic periodontitis was performed. Three months before the operation, according to the indications, endodontic treatment of the examined teeth was performed in some patients. Depending on the status of the dental pulp, the patients were divided into two groups: I – patients with vital teeth, II – patients with teeth after endodontic treatment. Before and after surgery, all patients underwent clinical examination, enzyme immunoassay of saliva remodeling markers and optical densitotomometry of periodontal bone to compare the results of treatment. Results. The OHI-S index showed a significant decrease in the mean value by 12 months of observation in both groups, which was statistically significant. Higher indices of the PI index by the end of the observation period were detected in the area of depulpated teeth. An equivalent dynamics of a decrease in the value of periodontal attachment according to the CAL index was revealed in both groups after treatment. The saliva showed an equivalent decrease in sRANKL, an increase in OPG by the end of the observation period, statistically insignificant when compared between groups. FGF-2 remained undetected at any of the observation periods in all groups. Densitotomometry data indicated an increase in the density characteristics of periodontal bone by the end of observations with a stable dependence on lower values in the area of depulpated teeth. Conclusions. In the saliva of patients, the processes of periodontal bone resorption were found to be statistically equivalent in intensity, regardless of the status of the dental pulp. The absence of FGF-2 content in saliva until the end of the observation period should be considered as an indicator of periodontal healing of vital and depulpated teeth by the formation of fibrous connective tissue. Dynamic densitotomometry of alveolar bone tissue revealed statistically significantly lower density characteristics in the area of teeth with a condition after endodontic treatment.

Inhibition of the Nav1.7 Channel in the Trigeminal Ganglion Relieves Pulpitis Inflammatory Pain

Pulpitis causes significant changes in the peripheral nervous system, which induce hyperalgesia. However, the relationship between neuronal activity and Nav1.7 expression following pulpal noxious pain has not yet been investigated in the trigeminal ganglion (TG). The aim of our study was to verify whether experimentally induced pulpitis activates the expression of Nav1.7 peripherally and the neuronal activities of the TGs can be affected by Nav1.7 channel inhibition. Acute pulpitis was induced through allyl isothiocyanate (AITC) application to the rat maxillary molar tooth pulp. Three days after AITC application, abnormal pain behaviors were recorded, and the rats were euthanized to allow for immunohistochemical, optical imaging, and western blot analyses of the Nav1.7 expression in the TG. A significant increase in AITC-induced pain-like behaviors and histological evidence of pulpitis were observed. In addition, histological and western blot data showed that Nav1.7 expressions in the TGs were significantly higher in the AITC group than in the naive and saline group rats. Optical imaging showed that the AITC group showed higher neuronal activity after electrical stimulation of the TGs. Additionally, treatment of ProTxII, selective Nav1.7 blocker, on to the TGs in the AITC group effectively suppressed the hyperpolarized activity after electrical stimulation. These findings indicate that the inhibition of the Nav1.7 channel could modulate nociceptive signal processing in the TG following pulp inflammation.

Age Estimation Based on Pulp–Tooth Volume Ratio of Anterior Teeth in Cone-Beam Computed Tomographic Images in a Selected Population: A Cross-Sectional Study

The present study aimed to investigate the correlation between chronological age and pulp–tooth volume ratio in anterior teeth using cone beam computed tomographic (CBCT) images and provide equations for age estimation based on pulp–tooth volume ratio. In this cross-sectional study, CBCT images of 312 anterior teeth of the maxilla and mandible were examineαd. The chronological age and the sex of the patients were recorded. Tooth volume and pulp volume of the anterior teeth were measured using Mimics software, and then tooth–pulp volume ratio was calculated. Statistical analysis of data was performed using chi-square test, independent T-test, Pearson’s correlation, and linear regression (α = 0.05). Finally, equations were made based on the results of the regression analysis for age estimation in general and for males and females. The age of the subjects ranged between 16 and 69 years (mean 40.6 ± 12.74). The pulp–tooth volume ratio of the maxillary teeth was generally higher than the mandibular teeth. A significant inverse relationship between age and pulp–tooth volume ratio was observed for all anterior teeth. (p < 0.05). The strongest correlation between age and pulp–tooth volume ratio was reported for mandibular central incisor (r = −0.58, p < 0.001) and the weakest was for mandibular lateral incisor (r = −0.36, p = 0.012). Age estimation was most accurate among females using the maxillary central incisors (p < 0.001) and among males using the mandibular central incisors and maxillary canines (p = 0.003). Pulp–tooth volume ratio of mandibular central incisors, maxillary lateral incisors, and maxillary canines in males and maxillary and mandibular central incisors, maxillary lateral incisors, and maxillary and mandibular canines in females can be used for age estimation.

The features of metabolism and structural organization of dental system under conditions of experimental insulin resistance

Peculiarities of calcium homeostasis, the activity of energy synthesis enzymes and structural organization of the dental system in rats under conditions of insulin resistance were analyzed. It was found that impaired glucose tolerance is manifested by a decreased mineralizing ability of hard tissues of alveolar processes and teeth, accompanied by a decrease in calcium content in erythrocyte mass and alveolar processes and an increase in acid phosphatase activity in blood serum. In rats with insulin resistance a decrease in the activity of lactate- and succinate dehydrogenase was detected. Degenerative changes were observed in the bone thickness of the dental area of insulin resistant rats, which were accompanied by the development of hypercellularity of the osteogenic layer of periosteum. Reactive changes in the tooth pulp were manifested by a decrease in the area of its loose connective tissue. Hyperplastic changes with the formation of unexpressed acanthotic bands developed in the oral mucosa of experimental animals, the thickness of the basal and granular layers increased against the background of a decrease in the prickle layer of epitheliocytes. In experimental group we observed a decrease in the nuclear cytoplasmic index and an increase in keratin formation in the epithelium. A narrowing of the lumen of capillaries and arterioles of the dental area, hyperplasia of endothelial cells and an increased accumulation of glycoproteins, especially in small arteries, were also detected in experimental group. Thus, altered carbohydrate metabolism leads to the metabolic changes of teeth supporting apparatus and oral mucosa, aggravating the course of insulin resistance with the development of dental system pathology.

MORPHOLOGICAL ASPECTS OF THE RELATIONSHIP BETWEEN PERIODONTAL TISSUES AND DENTAL PULP

Researchers are currently paying more and more attention to the combined pathology of the tooth pulp and periodontal. At the same time, the peculiarities of the structure of the alveolar bone, the cement of the tooth root and the root canal system that cause their interrelation and mutual influence remain insufficiently studied. The main elements of the connection between the pulp and periodontal tissues are not only apical openings and additional channels, but also other ways of interaction, the study of which is of great interest for understanding the features of pathogenesis, prognosis and treatment of combined diseases of the tooth pulp and periodontal. The aim of the study is to identify the topographical and anatomical features of the structure of the roots of teeth of different functional groups and the surfaces of their holes when studying on human cadaver material. The hypothesis of the study is that the number of openings of the Haversov channels and their localization in the moons are due to the functional affiliation of the teeth and they have their own characteristics. Material and methods. The study was conducted on jawbones with teeth obtained from embalmed corpses of adults of different genders and ages. After appropriate preparation, at the first stage, microscopy of the alveolar surfaces was performed, followed by morphometric analysis of the photos. At the second stage, scanning electron microscopy (SEM) of the surfaces of the roots of teeth previously extracted from the same wells of the jaws was performed. Results and discussion. All the preparations were systematized according to the number and topography of the identified holes of the Haversov channels. Because of the assessment of morphometric indicators, the number of holes, the density of their location and the diameter were determined, depending on the functional affiliation of the teeth. The analysis of microphotographs obtained because of SEM allowed us to determine the structural features of the surfaces of the roots of the teeth. Conclusions. The number, density and diameter of the holes of the Haversov channels are determined by the functional affiliation of the tooth. With the help of SEM, micro cracks and areas of root dentin exposure were found on the surfaces of the roots of the teeth for the first time, which can contribute to the translocation of pathogenic microbiota from the periodontal pocket to the pulp of the tooth and vice versa.

Influence of anticholinergic drugs on the development of an experimental periodontitis model

The endogenous microbiome of the oral cavity plays an essential role in the development of periodontal disease. It also has a significant pathogenic effect on the inner-vation of the oral cavity organs. The experimental determination of the effectiveness of various drugs is required for the effective treatment of periodontal disease, and this involves the creation of a model of experimental periodontitis. The objective of this series of studies was to determine the possibility of reproduction of the experimental model of periodontitis and the study of the effects of anticholinergic drugs on the development of an experimental periodontitis model. The reproduction of the experimental model of periodontitis was performed by injecting the gums of rats with solutions of pathogenic factors: lipopolysaccharide, hyaluronidase and trypsin. We aimed to study the effect of anticholinergic drugs (pilocarpine and atropine) on the development of an experimental model of periodontitis after the injection of a hyaluronidase solution (2 mg/ml) into the rats' gums. The study was performed on white Wistar rats. Elastase activity, malonic dialdehyde content, urease activity (bacterial contamination index), lysozyme activity (an indicator of nonspecific immunity), and catalase activity (an antioxidant enzyme) were determined in the homogenate of the studied tissues. The results of a comparative study of the effect of three pathogenic factors (lipopolysaccharide, hyaluronidase, and trypsin) on the activity of elastase in different tissues of experimental animals (gums, tooth pulp, serum, and gastric mucosa) showed that hyaluronidase has the greatest proinflammatory effect. The action of pilocarpine and atropine was determined with an underline experimental periodontitis model. It was shown that both anticholinergic drugs stimulate the inflammatory process in the periodontium and that anticholinergic drugs enhance the proinflammatory effect of hyaluronidase.

Orthopedic treatment with fixed dentures taking into account the distribution and causes of defects in dental hard tissues

The aim of this work was to analyze the causes of complications of orthopedic treatment of dentition defects with metal-ceramic crowns and non-removable metal-ceramic bridges. Materials and methods. A total of 140 patients aged 34 to 49 years were examined during the work, who underwent the production of both single metal-ceramic crowns and non-removable metal-ceramic bridges. All patients were divided into 2 groups (70 patients each): the first one included patients with vital abutment teeth, the second one – with depulped. The abutment teeth had a different functional state – they were filled, had wedge-shaped defects or increased abrasion. Results. Orthopedic treatment showed that the manufacture of solid metal-ceramic crowns on dental teeth, which were filled more than 1 year ago, led to complications in 18 of 35 (51.4 %) patients in this group, which is insignificantly (P ˃ 0.05) by 17.2 % less than with similar prosthetics on depulped teeth. Regarding the manufacture of bridges, the trend of more complications in prosthetics on devital teeth persists, because in group I we had 20 (57.7 %) patients with complications, and in group II – 29 (82.9 %) cases, which is significantly different at the level of P < 0.05. In prosthetics of vital teeth, defects of hard tissues of which are replaced by filling material immediately before prosthetics, single crowns of complications occurred in 3 patients, i.e. in 8.6 %, which is insignificantly (P ˃ 0.05) less by 5.6 % than in group II where complications were found in 5 (14.2 %) people. Analysis of orthopedic treatment of patients with included defects of dentitions with metal-ceramic bridge prostheses revealed that when using filled teeth (filled for 1 year before prosthetics) in the case, when they were depulped, as abutment, complications were observed in 9 people, i.e. in 25.7 % of this group. This indicator insignificantly (P ˃ 0.05) exceeds the identical one from the first group (14.3 %) by 11.4 %, therefore, there is a tendency to reduce tooth resistance to negative external and internal factors, which has not acquired statistical significance. Conclusions. The study found that the frequency of complications that occur after prosthetics with fixed dentures depends on the condition of the tooth pulp, because the viable pulp provides metabolism, renewal of tooth cells and response to any damage. The need to replace fillings that were made more than 1 year ago to reduce the risk of secondary caries has been proven. Defects of hard tissues in the form of a wedge-shaped defect and the increased attrition do not increase frequency of complications in prosthetics by fixed dentures.

ABO Genotyping from Pulp and Dentin Using DNA - A PCR Study

Background: Blood grouping has a major role in forensic science in the field of human identification. Aim of the Study: Aim of this study was to determine ABO genotyping from pulp and dentin using PCR method. Objectives: To determine PCR based blood grouping from the DNA isolated from tooth Pulp and Dentin. Materials and Meathods: Dental pulp tissue and Dentin was collected from 20 permanent teeth and DNA extraction was carried out from pulp using (modified proteinase –K method) and from dentin using phenol/chloroform(organic method). PCR procedure was carried out and samples were subjected to agarose gel electrophoresis and blood group was determined using specific PCR primers for ABO genotyping. Results: Among the 20 samples of pulp tissue, 17 samples showed accurate results in PCR method in comparison with slide agglutination method. Among the 20 samples of dentin, blood grouping from dentin was not possible as the quantity and purity of DNA from dentin samples were not optimal. Sensitivity of 85% and specificity of 50% was noticed from the samples of pulp. Conclusion: PCR was found to be an effective method in determination of blood group from teeth. Thus our study states that isolation of DNA can be done from both pulp and dentin and the blood grouping can be done from tooth pulp by PCR method. Hence PCR method can be used for identification of individuals which adds beneficiary value to forensic dentistry.

Mesenchymal stem cell applications in polycystic ovary syndrome treatment

Mesenchymal stem cells (MSCs) are highly capable of self-renewal and differentiation. They can be isolated from a variety of sources such as adipose tissue, bone marrow, umbilical cord, tooth pulp and can be cultured under in vitro conditions. MSCs have anti-inflammatory, anti-apoptotic, angiogenic, immunomodulatory and many more therapeutic effects due to the effects of paracrine factors they secrete. Today, mesenchymal stem cells are used for treatment in more than twenty diseases, from spinal cord injuries to diabetes. However, there is little mention in the literature of the use of these cells in female reproductive system diseases. In this review, a limited number of clinical and experimental studies on the use of mesenchymal stem cells in the treatment of polycystic ovary syndrome, which is very common in women, were examined and analyzed.