scholarly journals Effect of nitrogen limitation on growth, biochemical composition, and cell ultrastructure of the microalga Picocystis salinarum

2021 ◽  
Author(s):  
Ronald Tarazona Delgado ◽  
Mayara dos Santos Guarieiro ◽  
Paulo Wagnner Antunes ◽  
Sérvio Túlio Cassini ◽  
Haydee Montoya Terreros ◽  
...  
Keyword(s):  
Biochemical Composition ◽  
Nitrogen Limitation ◽  
Cell Ultrastructure

scholarly journals Effect of Nitrogen Limitation on Growth, Biochemical Composition and Cell Ultrastructure of the Microalga Picocystis Salinarum

2020 ◽  
Author(s):  
Ronald Tarazona Delgado ◽  
Mayara dos Santos Guarieiro ◽  
Paulo Wagnner Antunes ◽  
Sérvio Túlio Cassini ◽  
Haydee Montoya Terreros ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Content ◽  
Biochemical Composition ◽  
Nitrogen Limitation ◽  
Dry Weight ◽  
Cell Ultrastructure ◽  
Raw Material ◽  
Biodiesel Production ◽  
Content Increase ◽  
N Limitation

Abstract In recent years, biodiesel production has attracted worldwide attention due to the awareness of fossil fuel depletion, and microalgae biomass is considered a promising raw material for its formulation. The present study evaluated the effects of different levels of nitrogen limitation (37.5, 18.75, 9.375 mg L-1 NaNO3) on the growth, cell ultrastructure and biochemical composition of Picocystis salinarum as a potential raw material source for biodiesel. During a culture period of 20 days, the growth measurements were estimated, and cell density, dry weight and chlorophylls a, b content decreased with time as nitrogen limitation increase, however, carotenoids content increased. The high N limitation (9.375 mg L-1) had a highly significant effect on the accumulation of total lipid content (33.87% dry weight), carbohydrate content increase (30.98% dry weight), but protein content decrease (1.89% dry weight). The lipid content showed a differential FAME profile with high saturated fatty acid values (996.08 µg g-1 dry weight) mainly C16:0, compare with the unsaturated ones that showed low values under high N limitation. The gradual increase of lipid content was also corroborated by transmission electron microscopy images with lipid droplet cell formation. Therefore, evaluation of the algal culture conditions such as N limitation, as a strategy to maximize lipid content and improve the fatty acid profile in unexplored halophilic P. salinarum showed a potential biomass yield as a suitable candidate for biodiesel production.

scholarly journals Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea

2017 ◽  
Vol 27 ◽  
pp. 177-189 ◽  
Author(s):  
Fiz da Costa ◽  
Fabienne Le Grand ◽  
Claudie Quéré ◽  
Gaël Bougaran ◽  
Jean Paul Cadoret ◽  
...  
Keyword(s):  
Growth Phase ◽  
Biochemical Composition ◽  
Nitrogen Limitation ◽  
Tisochrysis Lutea

Nitrogen Limitation in Neochloris oleoabundans: A Reassessment of Its Effect on Cell Growth and Biochemical Composition

2013 ◽  
Vol 171 (7) ◽  
pp. 1775-1791 ◽  
Author(s):  
Adriana Garibay-Hernández ◽  
Rafael Vazquez-Duhalt ◽  
Leobardo Serrano-Carreón ◽  
Alfredo Martinez
Keyword(s):  
Cell Growth ◽  
Biochemical Composition ◽  
Nitrogen Limitation ◽  
Neochloris Oleoabundans

Effects of the pseudomonad phytotoxin coronatine on tomato leaf structure and ultrastructure

1995 ◽  
Vol 53 ◽  
pp. 862-863
Author(s):  
D.A. Palmer ◽  
C.L. Bender
Keyword(s):  
Pseudomonas Syringae ◽  
Membrane Integrity ◽  
Leaf Tissue ◽  
Cell Number ◽  
Cell Ultrastructure ◽  
Response To Treatment ◽  
Prominent Symptom ◽  
Chlorophylls A And B ◽  
Cell Dimensions ◽  
Structure And Ultrastructure

Coronatine is a non-host-specific phytotoxin produced by several members of the Pseudomonas syringae group of pathovars. The toxin acts as a virulence factor in P. syringae pv. tomato, allowing the organism to multiply to a higher population density and develop larger lesions than mutant strains unable to produce the toxin. The most prominent symptom observed in leaf tissue treated with coronatine is an intense spreading chlorosis; this has been attributed to a loss of chlorophylls a and b in tobacco. Coronatine's effects on membrane integrity and cell ultrastructure have not been previously investigated. The present study describes changes in tomato leaves in response to treatment with purified coronatine, infection by a coronatine-producing strain of P. syringae pv. tomato, and infection by a cor" mutant.In contrast to H2O-treated tissue, coronatine-treated tissue showed a diffuse chlorosis extending approximately 5 mm from the inoculation site. Leaf thickness, cell number, and cell dimensions were similar for both healthy and coronatine-treated, chlorotic tissue; however, the epidermal cell walls were consistently thicker in coronatine-treated leaves (Figs, la and lb).

Dynamics of the macromolecular composition of the biomass of microalgae in the morning under natural light. Model

2020 ◽  
pp. 45-52
Author(s):  
Alexander S. Lelekov ◽  
Anton V. Shiryaev
Keyword(s):  
Biochemical Composition ◽  
Initial Conditions ◽  
Natural Light ◽  
Photoautotrophic Growth ◽  
Structural Part ◽  
Basic Model ◽  
Microalgae Culture ◽  
Simulation Results ◽  
Structural Parts ◽  
Macromolecular Composition

The work is devoted to modeling the growth of optically dense microalgae cultures in natural light. The basic model is based on the idea of the two-stage photoautotrophic growth of microalgae. It is shown that the increase in the intensity of sunlight in the first half of the day can be described by a linear equation. Analytical equations for the growth of biomass of microalgae and its macromolecular components are obtained. As the initial conditions, it is assumed that at the time of sunrise, the concentration of reserve biomass compounds is zero. The simulation results show that after sunrise, the growth of the microalgae culture is due only to an increase in the reserve part of the biomass, while the structural part practically does not change over six hours. Changes in the ratio of the reserve and structural parts of the biomass indicate a change in the biochemical composition of cells.

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