Immobilization and bioactivity evaluation of FGF-1 and FGF-2 on powdered silicon-doped hydroxyapatite and their scaffolds for bone tissue engineering

2010 ◽  
Vol 22 (2) ◽  
pp. 405-416 ◽  
Author(s):  
María José Feito ◽  
Rosa María Lozano ◽  
María Alcaide ◽  
Cecilia Ramírez-Santillán ◽  
Daniel Arcos ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silicon Doped ◽  
Powdered Silicon

Upgrading Calcium Phosphate Scaffolds for Tissue Engineering Applications

2008 ◽  
Vol 377 ◽  
pp. 19-42 ◽  
Author(s):  
Sandra Sánchez-Salcedo ◽  
Daniel Arcos ◽  
Maria Vallet-Regí
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Clinical Performance ◽  
Bone Ingrowth ◽  
Calcium Phosphates ◽  
Synthesis Methods ◽  
Charge Imbalance ◽  
Silicon Doped

The research on ceramic scaffolds for bone tissue engineering is, nowadays, one of the newest and most attractive topics in the field of materials for biomedical applications. These scaffolds are aimed to provide supporting or even enhance the reparative capacity of body. Biphasic calcium phosphates (BCPs) and silicon doped BCP are very interesting candidates to be used as materials for scaffolds fabrication in bone tissue engineering. BCPs and silicon doped BCP consist of a mixture of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) or HA and α-tricalcium phosphate (α-TCP), respectively. For the regenerative purposes BCPs show better performance than HA because of the higher solubility of β-TCP compound, which facilitate the subsequent bone ingrowth in the implant. On the other, silicon doped BCP involve silicon that substituted into the apaptite crystal lattice for phosphorous with the subsequent charge imbalance. HA/α-TCP based bioceramics exhibits an important improvement of the bioactive behaviour with respect to non-substituted apatites. This work reviews the procedures to synthesise and fabricate scaffolds based on HA/β-TCP and silicon stabilised HA/α-TCP. Special attraction has been paid in the different synthesis methods and to the shaping of final scaffolds. By knowing the scaffold features at the crystallinity and macrostuctural level, the biocompatibility and clinical performance can be better understood, which will be also considered in this review.

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

PLGA+HA/βTCP scaffold incorporating simvastatin: a promising biomaterial for bone tissue engineering

2020 ◽  
Author(s):  
Mariane Beatriz Sordi ◽  
Ariadne Cristiane Cabral da Cruz ◽  
Águedo Aragones ◽  
Mabel Mariela Rodríguez Cordeiro ◽  
Ricardo de Souza Magini
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Thermal Analyses ◽  
Chemical Properties ◽  
Biological Properties ◽  
Scanning Calorimetry ◽  
Evaporation Technique ◽  
Porosity Analysis ◽  
Biphasic Ceramic

The aim of this study was to synthesize, characterize, and evaluate degradation and biocompatibility of poly(lactic-co-glycolic acid) + hydroxyapatite / β-tricalcium phosphate (PLGA+HA/βTCP) scaffolds incorporating simvastatin (SIM) to verify if this biomaterial might be promising for bone tissue engineering. Samples were obtained by the solvent evaporation technique. Biphasic ceramic particles (70% HA, 30% βTCP) were added to PLGA in a ratio of 1:1. Samples with SIM received 1% (m:m) of this medication. Scaffolds were synthesized in a cylindric-shape and sterilized by ethylene oxide. For degradation analysis, samples were immersed in PBS at 37 °C under constant stirring for 7, 14, 21, and 28 days. Non-degraded samples were taken as reference. Mass variation, scanning electron microscopy, porosity analysis, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetry were performed to evaluate physico-chemical properties. Wettability and cytotoxicity tests were conducted to evaluate the biocompatibility. Microscopic images revealed the presence of macro, meso, and micropores in the polymer structure with HA/βTCP particles homogeneously dispersed. Chemical and thermal analyses presented very similar results for both PLGA+HA/βTCP and PLGA+HA/βTCP+SIM. The incorporation of simvastatin improved the hydrophilicity of scaffolds. Additionally, PLGA+HA/βTCP and PLGA+HA/βTCP+SIM scaffolds were biocompatible for osteoblasts and mesenchymal stem cells. In summary, PLGA+HA/βTCP scaffolds incorporating simvastatin presented adequate structural, chemical, thermal, and biological properties for bone tissue engineering.

Bone tissue engineering and repair by gene therapy

10.2741/2724 ◽  
2008 ◽  
Vol 13 (13) ◽  
pp. 833 ◽  
Author(s):  
Volker, M. Betz
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue

Template-Mediated Biomineralization for Bone Tissue Engineering

2016 ◽  
Vol 12 (2) ◽  
pp. 103-123 ◽  
Author(s):  
Alexander Leiendecker ◽  
Steffen Witzleben ◽  
Margit Schulze ◽  
Edda Tobiasch
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue

Application of Bioreactors to Improve Functionality of Bone Tissue Engineering Constructs: A Systematic Review

2017 ◽  
Vol 12 (7) ◽  
Author(s):  
Hanieh Nokhbatolfoghahaei ◽  
Maryam Rezai Rad ◽  
Mohammad-Mehdi Khani ◽  
Shayan Shahriari ◽  
Nasser Nadjmi ◽  
...  
Keyword(s):  
Systematic Review ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue

scholarly journals Correlating cell morphology and osteoid mineralization relative to strain profile for bone tissue engineering applications

2007 ◽  
Vol 5 (25) ◽  
pp. 899-907 ◽  
Author(s):  
M.A Wood ◽  
Y Yang ◽  
E Baas ◽  
D.O Meredith ◽  
R.G Richards ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Morphology ◽  
Bone Cells ◽  
Calcium Deposition ◽  
Cell Behaviour ◽  
Local Strains ◽  
Strain Profile ◽  
Pore Model

A number of bone tissue engineering strategies use porous three-dimensional scaffolds in combination with bioreactor regimes. The ability to understand cell behaviour relative to strain profile will allow for the effects of mechanical conditioning in bone tissue engineering to be realized and optimized. We have designed a model system to investigate the effects of strain profile on bone cell behaviour. This simplified model has been designed with a view to providing insight into the types of strain distribution occurring across a single pore of a scaffold subjected to perfusion–compression conditioning. Local strains were calculated at the surface of the pore model using finite-element analysis. Scanning electron microscopy was used in secondary electron mode to identify cell morphology within the pore relative to local strains, while backscattered electron detection in combination with X-ray microanalysis was used to identify calcium deposition. Morphology was altered according to the level of strain experienced by bone cells, where cells subjected to compressive strains (up to 0.61%) appeared extremely rounded while those experiencing zero and tensile strain (up to 0.81%) were well spread. Osteoid mineralization was similarly shown to be dose dependent with respect to substrate strain within the pore model, with the highest level of calcium deposition identified in the intermediate zones of tension/compression.

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