Green synthesis of silver, iron and gold nanoparticles of lycopene extracted from tomato: their characterization and cytotoxicity against COLO320DM, HT29 and Hella cell

AbstractOur study aimed at development of Silver, Iron and Gold nanoparticles of Lycopene isolated from tomato by using green synthesis technique and to evaluate its anticancer potential against colorectal and cervical cancer. Lycopene was extracted by benzene extraction method and the silver, iron and gold nanoparticles were developed by green synthesis method. 1% aqueous extract of isolated Lycopene was mixed with 1% solutions of AgNO3, FeCl3 and HAuCl4 solutions and incubated at ambient temperature for 3–4 h separately and observed for the color change which is an indicative of formation of the nanoparticles. The prepared nanoparticles were characterized by FTIR, SEM, XRD analysis and evaluated for their antimicrobial potential. The cytotoxicity studies were carried out by in vitro assay like MTT, SRB and Tryphan blue method against Colo 320 DM, HT 29, and Hella. SEM showed nanosized particles of 50–100 nm range, whereas no antimicrobial activity was exhibited by the prepared nanoparticles. In MTT assay the LyAgNP showed maximum 41.41 ± 0.4124% inhibition against COLO320DM, whereas LyGNP exhibited 41.47 ± 0.4469% inhibition against HT 29 and LyAgNP showed 40.9 ± 0.6908% inhibition against Hella cells. In SRB assay LyAgNP showed maximum 82.68 ± 1.1798% inhibition against COLO320DM, whereas LyGNP exhibited maximum 91.21 ± 0.2372% inhibition against HT29 and 87.98 ± 0.5878% inhibition against Hella cells. In tryphan blue assay against COLO320DM, HT29 and Hella cells, the maximum inhibition exhibited by the prepared nanoparticles were observed as LyGNP 83.45 ± 0.4694%, LyAgNP 88.05 ± 0.1870% and LyAgNP65.47 ± 0.4766%. We conclude that the developed nanoparticles of Lycopene exhibited potential anticancer activity against Colorectal and cervical cancer cell as compared with pure Lycopene.

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GREEN SYNTHESIS OF GOLD NANOPARTICLES OF ISOLATED CITRUS BIOFLAVONOID FROM ORANGE: CHARACTERIZATION AND IN VITRO CYTOTOXICITY AGAINST COLON CANCER CELL LINES COLO 320DM AND HT29

INDIAN DRUGS ◽

10.53879/id.57.08.12514 ◽

2020 ◽

Vol 57 (08) ◽

pp. 61-69

Author(s):

D. S Randive ◽

K. P. Shejawal ◽

S. D Bhinge ◽

M. A Bhutkar ◽

P. D. Patil ◽

...

Keyword(s):

Colon Cancer ◽

Gold Nanoparticles ◽

Green Synthesis ◽

Cancer Cell ◽

Orange Peel ◽

In Vitro Cytotoxicity ◽

Colon Cancer Cell ◽

Cytotoxicity Studies ◽

Ht 29

The aim of the present research was to perform green synthesis of gold nanoparticles of isolated citrus bioflavonoid from Citrus sinensis (orange) peel extract and to evaluate its anticancer potential. Methanolic extract of orange peel was obtained by Soxhlet extraction and citrus bioflavonoid was isolated by using column chromatography. Gold nanoparticles were developed by green synthesis method, wherein 1 % aqueous solution of isolated citrus bioflavonoid were mixed with 1% solution of HAuCl4 and incubated at ambient temperature for 4 to 5 hours and observed for the color change which indicated formation of nanoparticles. Obtained gold nanoparticles were evaluated by UV visible spectra, FTIR, SEM, XRD analysis and for antimicrobial potential against E coli, S.aureus and P. aeruginosa. Cytotoxicity study was carried out by using in vitro assays, namely MTT, SRB and Tryphan blue assay, against colon cancer cell line Colo 320 DM, and HT 29. results of SEM showed that nanosized particles in the range of 80-100nm were formed. Results of cytotoxicity studies revealed that CBFGNP exhibited 72.28% inhibition, against Colo320 DM whereas pure CBF showed 70.46% inhibition. Against HT 29, CBFGNP exhibited 69.79% inhibition, whereas for MTT assay and SRB assay, CBFGNP showed 80.15% and 58.29% inhibition, respectively. Moreover, CBFGNP exhibited 90.29% and 85% non viability against Colo320 DM and HT29. Based on the results, it can be concluded that gold nanoparticles of citrus bioflavonoid (CBFGNP) exhibits more cytotoxicity against Colo320 DM and HT29 as compared to pure citrus bioflavonoid when assessed by three different in vitro cytotoxicity assays.

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Synthesis and Biological Evaluation of Amoxicillin Loaded Hybrid Material Composite Spheres Against Methicillin-Resistant Staphylococcus aureus

Current Pharmaceutical Biotechnology ◽

10.2174/1389201021666201221143537 ◽

2020 ◽

Vol 21 ◽

Author(s):

Muhammad Arfat Yameen ◽

Amir Zeb ◽

Raza E Mustafa ◽

Sana Mushtaq ◽

Nargis Aman ◽

...

Keyword(s):

Mtt Assay ◽

Hybrid Material ◽

Ag Nanoparticles ◽

Hybrid Composite ◽

Synthesis Method ◽

Biological Evaluation ◽

Vitro Assay ◽

Cytotoxicity Studies ◽

Material Composite

Background: Incoherent use of antibiotics has led toward resistance in MRSA, which is becoming multidrugresistant with high rate of virulence in the community and hospital settings. Objective: Synergistic anti-MRSA activity was investigated in this study for hybrid material composite spheres of amoxicillin, Ag nanoparticles and chitosan which were prepared by one-step synthesis method and various characterizations were performed. Methods: Antimicrobial-susceptibility assay on MRSA was achieved by disc diffusion and agar dilution techniques while agar well diffusion was used for hybrid composite spheres. The in vitro and cytotoxicity studies was done by skin abrasion mouse model and MTT assay on RD cell respectively. Results: All isolates were resistant with the tested antibiotics except vancomycin. MIC against MRSA showed high resistance with amoxicillin from 4 to 128 mg L-1. The mean diameter of chitosan spheres and Ag nanoparticles was 02 mm and 277 nm respectively. Morphology of spheres was uneven, varied, porous and irregular in SEM and Ag nanoparticles presence and formation was also seen in micrograph. No substantial interface among drug, nanoparticles and polymer was found in XRD and IR showed characteristic peaks of all compound in the formulation. The in vitro assay showed augmented anti-MRSA activity with amoxicillin loaded hybrid composite spheres (22-29 mm). A significant reduction in microbial burden (~6.5 log10 CFU ml-1) was seen in vivo with loaded hybrid composite spheres formulation. The MTT assay indicated no potential cytotoxicity with hybrid composite spheres. Conclusion: Synergistic effect, amoxicillin, new hybrid formulation, anti-MRSA activity, composite spheres. nanoparticles.

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Green synthesis of gold nanoparticles from Dunaliella salina, its characterization and in vitro anticancer activity on breast cancer cell line

Journal of Drug Delivery Science and Technology ◽

10.1016/j.jddst.2019.02.023 ◽

2019 ◽

Vol 51 ◽

pp. 164-176 ◽

Cited By ~ 16

Author(s):

Ankit Kumar Singh ◽

Ratnakar Tiwari ◽

Vikas Kumar Singh ◽

Prabhakar Singh ◽

Sk Riyazat Khadim ◽

...

Keyword(s):

Breast Cancer ◽

Gold Nanoparticles ◽

Green Synthesis ◽

Anticancer Activity ◽

Cell Line ◽

Breast Cancer Cell ◽

Breast Cancer Cell Line ◽

Dunaliella Salina ◽

Cancer Cell Line

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A COMPARATIVE ANTICANCER STUDY OF BIOSYNTHESIZED NANOPARTICLES, DOXORUBICIN & NANO-DOX CONJUGATE AGAINST CERVICAL CANCER HELA CELL LINE

10.36106/ijsr/2509427 ◽

2020 ◽

pp. 4-7

Author(s):

M. R. Kamala Priya ◽

Priya R. Iyer

Keyword(s):

Cervical Cancer ◽

Gold Nanoparticles ◽

Cell Death ◽

Cancer Therapy ◽

Hela Cell ◽

Cell Line ◽

In Vitro Cytotoxicity ◽

Hela Cell Line ◽

Vero Cell Line

Doxorubicin is the most common chemotherapy drug used in cancer therapy. Its usage is associated with various side-effects. In order to overcome the challenges in Doxorubicin administration, the present study has focussed on synthesizing a drug conjugate with biosynthesized gold nanoparticles and doxorubicin. The gold nanoparticles were biosynthesized using green extracts of medicinal plants with potential anticancer activities. The nanoparticle that possesses anticancer activity was conjugated with the drug for a combinatorial effect of the nanoparticles and the drug. The in vitro cytotoxicity was checked in Vero cell line through MTT assay. The in vitro anti proliferative effects were screened against cervical cancer in HeLa cell line. Fluorescence activated cell sorting analysis was carried out to detect the difference between live and dead cell populations. The preliminary confirmation was carried out in UV-VIS spectrophotometer. The morphological characterization was carried out by SEM and stability by Zeta potential. The IC50 of the nanocompounds demonstrated anti-proliferative activity against cervical cancer similar to the chemotherapeutic drug, Doxorubicin; additionally in a much lesser concentration of the drug. The in vitro cytotoxicity exhibited high viability of cells in Vero cell line with minimum viability of 80% in all the tested concentrations. There was a synergistic effect of the nanoparticles along with the drug; thereby an enhanced therapeutic efficiency was achieved. FACS analysis showed 36% of cell death in Dox treated HeLa cells whereas 96% of cell death in Nano-Dox treated HeLa cells. Nano-Dox conjugate has demonstrated high anticancer effects than drug alone Doxorubicin. Further biosynthesized nanomaterials based drug formulation can be developed as a potential strategy in cancer therapy.

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Green Synthesis of Gold Nanoparticles from Vitex negundo Leaf Extract to Inhibit Lipopolysaccharide-Induced Inflammation Through In Vitro and In Vivo

Journal of Cluster Science ◽

10.1007/s10876-019-01661-1 ◽

2019 ◽

Vol 31 (2) ◽

pp. 463-477 ◽

Cited By ~ 5

Author(s):

Ningaraju Sunayana ◽

Munawer Uzma ◽

Rudra Prasad Dhanwini ◽

Mellappa Govindappa ◽

Harischandra Sripathy Prakash ◽

...

Keyword(s):

Gold Nanoparticles ◽

Green Synthesis ◽

Leaf Extract ◽

Vitex Negundo

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Garlic, green tea and turmeric extracts-mediated green synthesis of silver nanoparticles: Phytochemical, antioxidant and in vitro cytotoxicity studies

Journal of Photochemistry and Photobiology B Biology ◽

10.1016/j.jphotobiol.2018.02.014 ◽

2018 ◽

Vol 180 ◽

pp. 243-252 ◽

Cited By ~ 54

Author(s):

D. Arumai Selvan ◽

D. Mahendiran ◽

R. Senthil Kumar ◽

A. Kalilur Rahiman

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Green Tea ◽

In Vitro Cytotoxicity ◽

Cytotoxicity Studies

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In vitro Antibacterial and Anticancer Potential of CeO2 Nanoparticles Prepared by Co-precipitation and Green Synthesis Method

Journal of Nanosciences Current Research ◽

10.4172/2572-0813.1000111 ◽

2017 ◽

Vol 02 (02) ◽

Cited By ~ 6

Author(s):

D. S Parvathya ◽

Venkatramanb BR

Keyword(s):

Green Synthesis ◽

Synthesis Method ◽

Ceo2 Nanoparticles ◽

Co Precipitation

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Optimisation of Folate-Mediated Liposomal Encapsulated Arsenic Trioxide for Treating HPV-Positive Cervical Cancer Cells In Vitro

International Journal of Molecular Sciences ◽

10.3390/ijms20092156 ◽

2019 ◽

Vol 20 (9) ◽

pp. 2156 ◽

Cited By ~ 5

Author(s):

Akhtar ◽

Ghali ◽

Wang ◽

Bell ◽

Li ◽

...

Keyword(s):

Cervical Cancer ◽

Folic Acid ◽

Cancer Cells ◽

Arsenic Trioxide ◽

Folate Receptor ◽

Hpv Infection ◽

Cytotoxicity Studies ◽

Cervical Cancer Cells ◽

Infected Cells

High-risk human papilloma virus (HPV) infection is directly associated with cervical cancer development. Arsenic trioxide (ATO), despite inducing apoptosis in HPV-infected cervical cancer cells in vitro, has been compromised by toxicity and poor pharmacokinetics in clinical trials. Therefore, to improve ATO’s therapeutic profile for HPV-related cancers, this study aims to explore the effects of length of ligand spacers of folate-targeted liposomes on the efficiency of ATO delivery to HPV-infected cells. Fluorescent ATO encapsulated liposomes with folic acid (FA) conjugated to two different PEG lengths (2000 Da and 5000 Da) were synthesised, and their cellular uptake was examined for HPV-positive HeLa and KB and HPV-negative HT-3 cells using confocal microscopy, flow cytometry, and spectrophotometer readings. Cellular arsenic quantification and anti-tumour efficacy was evaluated through inductively coupled plasma-mass spectrometry (ICP-MS) and cytotoxicity studies, respectively. Results showed that liposomes with a longer folic acid-polyethylene glycol (FA-PEG) spacer (5000 Da) displayed a higher efficiency in targeting folate receptor (FR) + HPV-infected cells without increasing any inherent cytotoxicity. Targeted liposomally delivered ATO also displayed superior selectivity and efficiency in inducing higher cell apoptosis in HPV-positive cells per unit of arsenic taken up than free ATO, in contrast to HT-3. These findings may hold promise in improving the management of HPV-associated cancers.

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