Development and Characterization of Phytoniosome Nano Vesicle Loaded with Aqueous Leaf Extracts of Justicia adhatoda and Psidium guajoava Against Dengue Virus (DEN-2)

2020 ◽  
Author(s):  
Dhanya K. Wilson ◽  
Govindarajan Shyamala ◽  
Manickam Paulpandi ◽  
Arul Narayanasamy ◽  
Karthik Siram ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Leaf Extracts ◽  
Justicia Adhatoda

scholarly journals Genomic investigation of a dengue virus outbreak in Thiès, Senegal, in 2018

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Amy Gaye ◽  
Tolla Ndiaye ◽  
Mouhamad Sy ◽  
Awa B. Deme ◽  
Alphonse B. Thiaw ◽  
...  
Keyword(s):  
West Africa ◽  
Dengue Virus ◽  
Clinical Signs ◽  
Health Concern ◽  
Public Health Concern ◽  
Local Circulation ◽  
Viral Genomes ◽  
Genomic Characterization ◽  
The City

AbstractDengue virus is a major and rapidly growing public health concern in tropic and subtropic regions across the globe. In late 2018, Senegal experienced its largest dengue virus outbreak to date, covering several regions. However, little is known about the genetic diversity of dengue virus (DENV) in Senegal. Here we report complete viral genomes from 17 previously undetected DENV cases from the city of Thiès. In total we identified 19 cases of DENV in a cohort of 198 individuals with fever collected in October and November 2018. We detected 3 co-circulating serotypes; DENV 3 was the most frequent accounting for 11/17 sequences (65%), 4 (23%) were DENV2 and 2 (12%) were DENV1. Sequences were most similar to recent sequences from West Africa, suggesting ongoing local circulation of viral populations; however, detailed inference is limited by the scarcity of available genomic data. We did not find clear associations with reported clinical signs or symptoms, highlighting the importance of testing for diagnosing febrile diseases. Overall, these findings expand the known range of DENV in Senegal, and underscore the need for better genomic characterization of DENV in West Africa.

scholarly journals DETECTION OF BIOACTIVE FRACTIONS OF JUSTICIA ADHATODA L. LEAVES

2013 ◽  
Vol 3 ◽  
pp. 388
Author(s):  
Faiza Rasheed ◽  
Keyword(s):  
Crude Oil ◽  
Metal Ions ◽  
Dietary Fiber ◽  
Total Alkaloid ◽  
Antioxidant Activities ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Essential Metal ◽  
Justicia Adhatoda

In vitro antibacterial and antioxidant activities of various leaf extracts of Justicia adhatoda L. (locally known as Bhaikar) were assessed. The leaves were also subjected to various phytochemical analysis. Results revealed that leaves of J. adhatoda L. contain significant amount of total alkaloid, phenols flavonoid, saponins, tannins , protein, crude oil, dietary fiber, essential and non essential metal ions. The methanol, ethanol, butanol, chloroform and n-hexane leaf extracts of J

scholarly journals Textural Characterization of Dietetic Amrakhand Prepared by using Stevia Leaf Extracts Powder

2021 ◽  
Author(s):  
Jairaj C. Tondare ◽  
Ashok S. Hembade
Keyword(s):  
Leaf Extract ◽  
Sugar Content ◽  
Control Sample ◽  
Textural Properties ◽  
Leaf Extracts ◽  
Health Consciousness ◽  
Textural Characterization ◽  
High Sugar Content ◽  
Insulin Dependent Diabetic

Background: The name Amrakhand comes from two words ‘aam’ and ‘shrikhand’ literally meaning mango shrikhand. It is popular mango yoghurt in Maharashtra and Gujarat. Amrakhand contains high sugar content, but in the context of health consciousness refined carbohydrate is necessary to curb in the diet of both insulin and non-insulin dependent diabetic patient. Hence dietetic Amrakhand was prepared using stevia to study its effect and suitability as a natural sweetener.Methods: Amrakhand prepared using different proportions of sucrose and powdered stevia leaf extract. The proportions used are 30:70 (T1), 25:75 (T2), 20: 80 (T3), 15:85 (T4), 10:90 (T5) with control sample 100:0 (T0). Textural properties of dietetic Amrakhand such as hardness, consistency, index of viscosity and cohesiveness were evaluated by using a texture analyzer (TA-XT2I, Stable Micro System, UK).Result: Textural parameters reveals that effect of increasing the concentration level of powdered stevia leaf extract, decreases the hardness, consistency, cohesiveness and index of viscosity values of the product. Whereas good sensory results were found at 70% (T1) of sucrose replacement by stevia leaf extract in dietetic Amrakhand. Hence, present paper focused on evaluation of textural characterization of dietetic Amrakhand prepared by using Stevia leaf extracts powder.

scholarly journals A versatile reporter system to monitor virus infected cells and its application to dengue virus and SARS-CoV-2

2020 ◽  
Author(s):  
Felix Pahmeier ◽  
Christoper J Neufeldt ◽  
Berati Cerikan ◽  
Vibhu Prasad ◽  
Costantin Pape ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Viral Replication ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Live Cell ◽  
Reporter System ◽  
Infected Cells ◽  
Positive Strand Rna

ABSTRACTPositive-strand RNA viruses have been the etiological agents in several major disease outbreaks over the last few decades. Examples of that are flaviviruses, such as dengue virus and Zika virus that cause millions of yearly infections and spread around the globe, and coronaviruses, such as SARS-CoV-2, which is the cause of the current pandemic. The severity of outbreaks caused by these viruses stresses the importance of virology research in determining mechanisms to limit virus spread and to curb disease severity. Such studies require molecular tools to decipher virus-host interactions and to develop effective interventions. Here, we describe the generation and characterization of a reporter system to visualize dengue virus and SARS-CoV-2 replication in live cells. The system is based on viral protease activity causing cleavage and nuclear translocation of an engineered fluorescent protein that is expressed in the infected cells. We show the suitability of the system for live cell imaging and visualization of single infected cells as well as for screening and testing of antiviral compounds. Given the modular building blocks, the system is easy to manipulate and can be adapted to any virus encoding a protease, thus offering a high degree of flexibility.IMPORTANCEReporter systems are useful tools for fast and quantitative visualization of viral replication and spread within a host cell population. Here we describe a reporter system that takes advantage of virus-encoded proteases that are expressed in infected cells to cleave an ER-anchored fluorescent protein fused to a nuclear localization sequence. Upon cleavage, the fluorescent protein translocates to the nucleus, allowing for rapid detection of the infected cells. Using this system, we demonstrate reliable reporting activity for two major human pathogens from the Flaviviridae and the Coronaviridae families: dengue virus and SARS-CoV-2. We apply this reporter system to live cell imaging and use it for proof-of-concept to validate antiviral activity of a nucleoside analogue. This reporter system is not only an invaluable tool for the characterization of viral replication, but also for the discovery and development of antivirals that are urgently needed to halt the spread of these viruses.

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