Regurgitant Derived From the Tea Geometrid Ectropis obliqua Suppresses Wound-Induced Polyphenol Oxidases Activity in Tea Plants

Polyphenol oxidases (PPOs) have been reported to play an important role in protecting plants from attacks by herbivores. Though PPO genes in other plants have been extensively studied, research on PPO genes in the tea plant (Camellia sinensis) is lacking. In particular, which members of the PPO gene family elicit the defense response of the tea plant are as yet unknown. Here, two new PPO genes, CsPPO1 and CsPPO2, both of which had high identity with PPOs from other plants, were obtained from tea leaves. The full length of CsPPO1 contained an open reading frame (ORF) of 1740 bp that encoded a protein of 579 amino acids, while CsPPO2 contained an ORF of 1788 bp that encoded a protein of 595 amino acids. The deduced CsPPO1 and CsPPO2 proteins had calculated molecular masses of 64.6 and 65.9 kDa; the isoelectric points were 6.94 and 6.48, respectively. The expression products of recombinant CsPPO1 and CsPPO2 in Escherichia coli were about 91 and 92 kDa, respectively, but the recombinant proteins existed in the form of an inclusion body. Whereas CsPPO1 is highly expressed in stems, CsPPO2 is highly expressed in roots. Further results showed that the expression of CsPPO1 and CsPPO2 was wound- and Ectropis obliqua-induced, and that regurgitant, unlike treatment with wounding plus deionized water, significantly upregulated the transcriptional expression of CsPPO2 but not of CsPPO1. The difference between regurgitant and wounding indicates that CsPPO2 may play a more meaningful defensive role against E. obliqua than CsPPO1. Meanwhile, we found the active component(s) of the regurgitant elicited by the expression of CsPPO may contain small molecules (under 3-kDa molecular weight). These conclusions advance the understanding of the biological function of two new PPO genes and show that one of these, CsPPO2, may be a promising gene for engineering tea plants that are resistant to E. obliqua.

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Responses of Tea Plants to Climate Change: From Molecules to Ecosystems

10.3389/978-2-88966-368-2 ◽

2021 ◽

Keyword(s):

Climate Change ◽

Tea Plants

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Changes of Tea Plants’ Growth according to the Type of Sequential Double Shading

The Korean Tea Society ◽

10.29225/jkts.2020.26.1.57 ◽

2020 ◽

Vol 26 (1) ◽

pp. 51-57

Author(s):

Doobo Shim ◽

Mi Jin Kwon ◽

Eun-Ju Kang ◽

Kyung Hwan Cho ◽

Yong-Hwi Son ◽

...

Keyword(s):

Tea Plants

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(E)-Nerolidol is a volatile signal that induces defenses against insects and pathogens in tea plants

Horticulture Research ◽

10.1038/s41438-020-0275-7 ◽

2020 ◽

Vol 7 (1) ◽

Cited By ~ 7

Author(s):

Shenglong Chen ◽

Liping Zhang ◽

Xiaoming Cai ◽

Xin Li ◽

Lei Bian ◽

...

Keyword(s):

Tea Plants

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Effects of polyphenol oxidases on proteolysis and lipolysis during ensiling of Moringa oleifera leaves with or without pyrocatechol

Animal Feed Science and Technology ◽

10.1016/j.anifeedsci.2021.114870 ◽

2021 ◽

Vol 275 ◽

pp. 114870

Author(s):

Cheng Wang ◽

Ruiqi Pian ◽

Xiaoyang Chen ◽

Qing Zhang

Keyword(s):

Moringa Oleifera ◽

Polyphenol Oxidases ◽

Moringa Oleifera Leaves

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Biochemical characterization of specific Alanine Decarboxylase (AlaDC) and its ancestral enzyme Serine Decarboxylase (SDC) in tea plants (Camellia sinensis)

BMC Biotechnology ◽

10.1186/s12896-021-00674-x ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Peixian Bai ◽

Liyuan Wang ◽

Kang Wei ◽

Li Ruan ◽

Liyun Wu ◽

...

Keyword(s):

Gene Duplication ◽

Camellia Sinensis ◽

Biochemical Characterization ◽

Specific Activity ◽

Protein Sequences ◽

Biochemical Properties ◽

High Similarity ◽

New Gene ◽

Tea Plants

Abstract Background Alanine decarboxylase (AlaDC), specifically present in tea plants, is crucial for theanine biosynthesis. Serine decarboxylase (SDC), found in many plants, is a protein most closely related to AlaDC. To investigate whether the new gene AlaDC originate from gene SDC and to determine the biochemical properties of the two proteins from Camellia sinensis, the sequences of CsAlaDC and CsSDC were analyzed and the two proteins were over-expressed, purified, and characterized. Results The results showed that exon-intron structures of AlaDC and SDC were quite similar and the protein sequences, encoded by the two genes, shared a high similarity of 85.1%, revealing that new gene AlaDC originated from SDC by gene duplication. CsAlaDC and CsSDC catalyzed the decarboxylation of alanine and serine, respectively. CsAlaDC and CsSDC exhibited the optimal activities at 45 °C (pH 8.0) and 40 °C (pH 7.0), respectively. CsAlaDC was stable under 30 °C (pH 7.0) and CsSDC was stable under 40 °C (pH 6.0–8.0). The activities of the two enzymes were greatly enhanced by the presence of pyridoxal-5′-phosphate. The specific activity of CsSDC (30,488 IU/mg) was 8.8-fold higher than that of CsAlaDC (3467 IU/mg). Conclusions Comparing to CsAlaDC, its ancestral enzyme CsSDC exhibited a higher specific activity and a better thermal and pH stability, indicating that CsSDC acquired the optimized function after a longer evolutionary period. The biochemical properties of CsAlaDC might offer reference for theanine industrial production.

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Spring Frost Damage to Tea Plants Can Be Identified with Daily Minimum Air Temperatures Estimated by MODIS Land Surface Temperature Products

Remote Sensing ◽

10.3390/rs13061177 ◽

2021 ◽

Vol 13 (6) ◽

pp. 1177

Author(s):

Peijuan Wang ◽

Yuping Ma ◽

Junxian Tang ◽

Dingrong Wu ◽

Hui Chen ◽

...

Keyword(s):

Surface Temperature ◽

Air Temperature ◽

Land Surface Temperature ◽

Land Surface ◽

Frost Damage ◽

Estimation Model ◽

Spring Frost ◽

Tea Plants ◽

Elevation Model ◽

Minimum Air Temperature

Tea (Camellia sinensis) is one of the most dominant economic plants in China and plays an important role in agricultural economic benefits. Spring tea is the most popular drink due to Chinese drinking habits. Although the global temperature is generally warming, spring frost damage (SFD) to tea plants still occurs from time to time, and severely restricts the production and quality of spring tea. Therefore, monitoring and evaluating the impact of SFD to tea plants in a timely and precise manner is a significant and urgent task for scientists and tea producers in China. The region designated as the Middle and Lower Reaches of the Yangtze River (MLRYR) in China is a major tea plantation area producing small tea leaves and low shrubs. This region was selected to study SFD to tea plants using meteorological observations and remotely sensed products. Comparative analysis between minimum air temperature (Tmin) and two MODIS nighttime land surface temperature (LST) products at six pixel-window scales was used to determine the best suitable product and spatial scale. Results showed that the LST nighttime product derived from MYD11A1 data at the 3 × 3 pixel window resolution was the best proxy for daily minimum air temperature. A Tmin estimation model was established using this dataset and digital elevation model (DEM) data, employing the standard lapse rate of air temperature with elevation. Model validation with 145,210 ground-based Tmin observations showed that the accuracy of estimated Tmin was acceptable with a relatively high coefficient of determination (R2 = 0.841), low root mean square error (RMSE = 2.15 °C) and mean absolute error (MAE = 1.66 °C), and reasonable normalized RMSE (NRMSE = 25.4%) and Nash–Sutcliffe model efficiency (EF = 0.12), with significantly improved consistency of LST and Tmin estimation. Based on the Tmin estimation model, three major cooling episodes recorded in the "Yearbook of Meteorological Disasters in China" in spring 2006 were accurately identified, and several highlighted regions in the first two cooling episodes were also precisely captured. This study confirmed that estimating Tmin based on MYD11A1 nighttime products and DEM is a useful method for monitoring and evaluating SFD to tea plants in the MLRYR. Furthermore, this method precisely identified the spatial characteristics and distribution of SFD and will therefore be helpful for taking effective preventative measures to mitigate the economic losses resulting from frost damage.

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