Degradation of Poly(acrylic acid)s with Phenolic Side-Chains by Manganese Peroxidase from White Rot Fungi

2005 ◽  
Vol 13 (4) ◽  
pp. 357-363 ◽  
Author(s):  
Shinji Ito ◽  
Yasunari Okada ◽  
Hirofumi Hirai ◽  
Tomoaki Nishida
Keyword(s):  
Acrylic Acid ◽  
Manganese Peroxidase ◽  
White Rot Fungi ◽  
White Rot ◽  
Side Chains ◽  
Poly Acrylic Acid

Production of laccase and manganese peroxidase by white-rot fungi from sugarcane bagasse in solid bed: Use for dyes decolourisation

Sugar Tech ◽  
2008 ◽  
Vol 10 (3) ◽  
pp. 260-264 ◽  
Author(s):  
Gilda Guerra ◽  
Osmel Domínguez ◽  
Miguel Ramos-Leal ◽  
Ana M. Manzano ◽  
María I. Sánchez ◽  
...  
Keyword(s):  
Sugarcane Bagasse ◽  
Manganese Peroxidase ◽  
White Rot Fungi ◽  
White Rot

scholarly journals Functional Expression and One-Step Protein Purification of Manganese Peroxidase 1 (rMnP1) from Phanerochaete chrysosporium Using the E. coli-Expression System

2020 ◽  
Vol 21 (2) ◽  
pp. 416
Author(s):  
Angel De La Cruz Pech-Canul ◽  
Javier Carrillo-Campos ◽  
María de Lourdes Ballinas-Casarrubias ◽  
Rosa Lidia Solis-Oviedo ◽  
Selena Karina Hernández-Rascón ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Protein Purification ◽  
Phanerochaete Chrysosporium ◽  
Manganese Peroxidase ◽  
Expression System ◽  
White Rot Fungi ◽  
Functional Expression ◽  
White Rot ◽  
E Coli ◽  
One Step

Manganese peroxidases (MnP) from the white-rot fungi Phanerochaete chrysosporium catalyse the oxidation of Mn2+ to Mn3+, a strong oxidizer able to oxidize a wide variety of organic compounds. Different approaches have been used to unravel the enzymatic properties and potential applications of MnP. However, these efforts have been hampered by the limited production of native MnP by fungi. Heterologous expression of MnP has been achieved in both eukaryotic and prokaryotic expression systems, although with limited production and many disadvantages in the process. Here we described a novel molecular approach for the expression and purification of manganese peroxidase isoform 1 (MnP1) from P. chrysosporium using an E. coli-expression system. The proposed strategy involved the codon optimization and chemical synthesis of the MnP1 gene for optimised expression in the E. coli T7 shuffle host. Recombinant MnP1 (rMnP1) was expressed as a fusion protein, which was recovered from solubilised inclusion bodies. rMnP1 was purified from the fusion protein using intein-based protein purification techniques and a one-step affinity chromatography. The designated strategy allowed production of an active enzyme able to oxidize guaiacol or Mn2+.

Nanoporous Ultra-Low-κ Films Prepared from Fluorinated Polyimide with Grafted Poly(acrylic acid) Side Chains

2004 ◽  
Vol 16 (1) ◽  
pp. 54-57 ◽  
Author(s):  
W.-C. Wang ◽  
R. H. Vora ◽  
E.-T. Kang ◽  
K.-G. Neoh ◽  
C.-K. Ong ◽  
...  
Keyword(s):  
Acrylic Acid ◽  
Side Chains ◽  
Fluorinated Polyimide ◽  
Poly Acrylic Acid

Large scale production of manganese-peroxidase using agaric white-rot fungi

2002 ◽  
Vol 30 (4) ◽  
pp. 556-561 ◽  
Author(s):  
J. Nüske ◽  
K. Scheibner ◽  
U. Dornberger ◽  
R. Ullrich ◽  
M. Hofrichter
Keyword(s):  
Manganese Peroxidase ◽  
Large Scale ◽  
White Rot Fungi ◽  
White Rot ◽  
Scale Production ◽  
Large Scale Production
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