Production of laccase and manganese peroxidase by white-rot fungi from sugarcane bagasse in solid bed: Use for dyes decolourisation

Sugar Tech ◽  
2008 ◽  
Vol 10 (3) ◽  
pp. 260-264 ◽  
Author(s):  
Gilda Guerra ◽  
Osmel Domínguez ◽  
Miguel Ramos-Leal ◽  
Ana M. Manzano ◽  
María I. Sánchez ◽  
...  
Keyword(s):  
Sugarcane Bagasse ◽  
Manganese Peroxidase ◽  
White Rot Fungi ◽  
White Rot

scholarly journals Functional Expression and One-Step Protein Purification of Manganese Peroxidase 1 (rMnP1) from Phanerochaete chrysosporium Using the E. coli-Expression System

2020 ◽  
Vol 21 (2) ◽  
pp. 416
Author(s):  
Angel De La Cruz Pech-Canul ◽  
Javier Carrillo-Campos ◽  
María de Lourdes Ballinas-Casarrubias ◽  
Rosa Lidia Solis-Oviedo ◽  
Selena Karina Hernández-Rascón ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Protein Purification ◽  
Phanerochaete Chrysosporium ◽  
Manganese Peroxidase ◽  
Expression System ◽  
White Rot Fungi ◽  
Functional Expression ◽  
White Rot ◽  
E Coli ◽  
One Step

Manganese peroxidases (MnP) from the white-rot fungi Phanerochaete chrysosporium catalyse the oxidation of Mn2+ to Mn3+, a strong oxidizer able to oxidize a wide variety of organic compounds. Different approaches have been used to unravel the enzymatic properties and potential applications of MnP. However, these efforts have been hampered by the limited production of native MnP by fungi. Heterologous expression of MnP has been achieved in both eukaryotic and prokaryotic expression systems, although with limited production and many disadvantages in the process. Here we described a novel molecular approach for the expression and purification of manganese peroxidase isoform 1 (MnP1) from P. chrysosporium using an E. coli-expression system. The proposed strategy involved the codon optimization and chemical synthesis of the MnP1 gene for optimised expression in the E. coli T7 shuffle host. Recombinant MnP1 (rMnP1) was expressed as a fusion protein, which was recovered from solubilised inclusion bodies. rMnP1 was purified from the fusion protein using intein-based protein purification techniques and a one-step affinity chromatography. The designated strategy allowed production of an active enzyme able to oxidize guaiacol or Mn2+.

Bioconversion of sugarcane bagasse with white rot fungi

1988 ◽  
Vol 10 (5) ◽  
pp. 369-372 ◽  
Author(s):  
Neelam Kewalramani ◽  
D. N. Kamra ◽  
D. Lall ◽  
N. N. Pathak
Keyword(s):  
Sugarcane Bagasse ◽  
White Rot Fungi ◽  
White Rot

Sugarcane bagasse degradation and characterization of three white-rot fungi

2013 ◽  
Vol 131 ◽  
pp. 443-451 ◽  
Author(s):  
Xiu Qin Dong ◽  
Jin Shui Yang ◽  
Ning Zhu ◽  
En Tao Wang ◽  
Hong Li Yuan
Keyword(s):  
Sugarcane Bagasse ◽  
White Rot Fungi ◽  
White Rot

Large scale production of manganese-peroxidase using agaric white-rot fungi

2002 ◽  
Vol 30 (4) ◽  
pp. 556-561 ◽  
Author(s):  
J. Nüske ◽  
K. Scheibner ◽  
U. Dornberger ◽  
R. Ullrich ◽  
M. Hofrichter
Keyword(s):  
Manganese Peroxidase ◽  
Large Scale ◽  
White Rot Fungi ◽  
White Rot ◽  
Scale Production ◽  
Large Scale Production

scholarly journals Lignin-degrading activity and ligninolytic enzymes of different white-rot fungi: effects of manganese and malonate

1997 ◽  
Vol 75 (1) ◽  
pp. 61-71 ◽  
Author(s):  
Tamara Vares ◽  
Annele Hatakka
Keyword(s):  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Growth Conditions ◽  
Fungal Species ◽  
White Rot ◽  
Trametes Hirsuta ◽  
Air Atmosphere ◽  
Trametes Trogii

Ten species of white-rot fungi, mainly belonging to the family Polyporaceae (Basidiomycotina), were studied in terms of their ability to degrade14C-ring labelled synthetic lignin and secrete ligninolytic enzymes in liquid cultures under varying growth conditions. Lignin mineralization by the fungi in an air atmosphere did not exceed 14% within 29 days. Different responses to the elevated Mn2+concentration and the addition of a manganese chelator (sodium malonate) were observed among various fungal species. This could be related with the utilization of either lignin peroxidase (LiP) or manganese peroxidase (MnP) for lignin depolymerization, i.e., some fungi apparently had an LiP-dominating ligninolytic system and others an MnP-dominating ligninolytic system. The LiP isoforms were purified from Trametes gibbosa and Trametes trogii. Isoelectric focusing of purified ligninolytic enzymes revealed the expression of numerous MnP isoforms in Trametes gibbosa, Trametes hirsuta, Trametes trogii, and Abortiporus biennis grown under a high (50-fold) Mn2+level (120 μM) with the addition of the chelator. In addition, two to three laccase isoforms were detected. Key words: white-rot fungi, lignin degradation, lignin peroxidase, manganese peroxidase, manganese, malonate.

scholarly journals Chemical composition and in-vitro digestibility of sugarcane bagasse and rice husk treated with three strains of white rot fungi and effective microorganism

2019 ◽  
Vol 35 (1) ◽  
pp. 71-83
Author(s):  
Regasa Begna ◽  
Mengistu Urge ◽  
Tegene Negesse ◽  
Getechewu Animut
Keyword(s):  
Chemical Composition ◽  
Sugarcane Bagasse ◽  
Rice Husk ◽  
White Rot Fungi ◽  
Neutral Detergent Fiber ◽  
White Rot ◽  
In Vitro Digestibility ◽  
Biological Treatments ◽  
Effective Microorganism

A study was conducted to evaluate the effect of biological treatments of sugarcane bagasse (SCB) and rice husk (RH) with three strains of white-rot fungi (WRF) (Pleurotusostreatus (Po), Pleurotusflorida (Pf) and Trichodermaviride (Tv) and effective microorganism (EM) on the chemical composition and in-vitro digestibility. The experiment consisted of 2x5 factorial arrangements, two levels of feed (SCB and RH) and five levels of biological treatments (Control, Po, Pf, Tv, and EM).Treatment of RH with EM, Tv, Po and Pf, significantly increased crude protein content from 7.90% in untreated to 7.92, 10.46, 10.61 and 11.35%, respectively. The corresponding increase in CP% of sugarcane from 2.61% was 3.41, 5.96, 5.89 and 5.95%.Treatments significantly (P<0.001) decreased neutral detergent fiber, acid detergent fiber, acid detergent lignin cellulose and hemicelluloses contents with the lowest value recorded for Tv. The IVOMD, IVDMD and metabolizable energy (ME) were significantly (P<0.001) increased. In conclusion, the study indicates that treatment of RH with Trichodermaviride and SCB with EM is more effective than others in improving the nutritive value of the roughages. We suggest evaluation of the treated roughages on animal performance.

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