Investigation of radiolabelled chitosan nanoparticles bearing Cefpodoxime Proxetil, and in vitro antibacterial effect on Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli

2020 ◽  
Vol 326 (3) ◽  
pp. 1551-1558
Author(s):  
Derya Özel ◽  
Fatma Yurt
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Chitosan Nanoparticles ◽  
Gram Positive ◽  
Gram Negative ◽  
Cefpodoxime Proxetil

scholarly journals Antibacterial effect of the hydroalcoholic extract of Mauritia flexuosa leaves on gram-negative and gram-positive bacteria

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 1487
Author(s):  
Ana Sandoval Vergara ◽  
Marlon Farfán Córdova ◽  
Marco Leoncio Salazar Castillo ◽  
Icela Marissa Rodríguez Haro ◽  
Ana Paula Vizconde Rodríguez
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Hydroalcoholic Extract ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Extract Concentration ◽  
Mauritia Flexuosa

Background: Plant-derived compounds are sometimes used as substitutes for pharmaceuticals. Mauritia flexuosa is a palm tree that is widely distributed in South America, especially in the Amazon region. The San Martín region of Peru, in which this species of the Arecaceae family is found, has great biological diversity and there is economic potential in the utilization of natural resources in the region. Methods: In this study, the antibacterial effect of the hydroalcoholic extract of Mauritia flexuosa leaves was evaluated for gram-positive bacteria Staphylococcus aureus ATCC 25923 and Bacillus subtilis ATCC 6633 and gram-negative Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Salmonella enterica subsp. enterica ser. Typhi ATCC 11011. Mauritia flexuosa leaves were used to prepare concentrations of 10, 20, 40 and 60mg/ml. Phytochemical analysis was performed to identify secondary metabolites in the plants. For the experiment, 10 Mueller-Hinton agar plates were prepared and 1ml of bacterial inoculum, standardized to 0.5 McFarland, was added to each plate. The hydroalcoholic extract was added via the diffusion method, making five holes of 5mm each (four with extract concentrations and one with distilled water as a control group), and the plates were incubated for 24 hours at 36°C. The inhibition halo was measured in mm using a digital vernier caliper. Results: For gram-negative bacteria, an antibacterial effect was demonstrated for Pseudomonas aeruginosa only, at an extract concentration of 60mg/ml, with an inhibition halo of 14.8 mm. For gram-positive bacteria Baccillus subtilis and Staphylococcus aureus, an antibacterial effect was demonstrated at an extract concentration of 60mg/ml, with inhibition halos of 13.2mm and 15.4mm in diameter, respectively. Conclusion: It can be concluded that the hydroalcoholic extract of Mauritia flexuosa does not inhibit bacterial growth for gram-negative bacteria Salmonella Typhi and Escherichia coli.

scholarly journals IN VITRO AND IN SILICO ANTIMICROBIAL STUDY OF STANNANE OF PYRIDOXAL 5-PHOSPHATE

2017 ◽  
Vol 9 (2) ◽  
pp. 145
Author(s):  
Rohit Babu Aniyery ◽  
Anita Gupta ◽  
Prashant Singh ◽  
Sanju .
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
In Silico ◽  
Research Work ◽  
The Novel ◽  
Gram Positive ◽  
Antibacterial Study ◽  
Gram Negative ◽  
Novel Complex

<p><strong>Objective</strong>:<strong> </strong>The main objective of this research work is to synthesize a novel stannane of pyridoxal 5-phosphateand to study its antibacterial property.</p><p><strong>Methods: </strong>Conventional method was used to synthesize the stannane of pyridoxal 5-phosphateand its characterization was carried using UV-visible and <sup>1</sup>H NMR. The antibacterial study was carried against, <em>Staphylococcus aureus </em>(gram positive) and <em>Escherichia coli 1610</em>(gram negative) using well diffusion method. <em>In silico</em> antimicrobial was carried out using computational software iGemDock v2.1 tool.(Graphical Drug Design system for Docking, Screening, and Post-analysis), computational docking was carried out using different PDB (Protein Data Bank) files (2I42, 3EOO, 3D2U and 3D2Y). The structure was optimized prior docking using Gaussian software, and the method followed was Energy (Ground state) Hartree-Fork.</p><p><strong>Results: </strong>It was observed that the stannane of pyridoxal 5-phosphateinhibited bacterial growth of <em>Staphylococcus aureus</em> (gram positive) <em>and Escherichia coli 1610 </em>(gram negative) <em>in vitro</em> antibacterial study. The complex has shown good docking results on almost all the receptors, with interaction supporting the fitting of the drug to the target molecules. The novel complex has shown good antibacterial activity (theoretically) in insilico studies. It was found to having a good potency to efficiently inhibit the microbes <em>Burkholderia pseudomallei, Human cytomegalovirus</em>,<em> Yersinia enterocolitica and Escherichia coli,</em> based on the interaction profile. The synthesized stannane was found to be effective in halting the undesirable effects of selected PDB files.</p><p><strong>Conclusion</strong>:<strong> </strong>On the basis of the above findings in the present research work, the novel complex was found to be a good antimicrobial agent and our future studies will aim design of novel selective and potent inhibitors. Further <em>in vitro</em> studies of this compound against these bacteria will lead to a new pathway to a novel antibacterial drug discovery.</p>

scholarly journals Screening of Escherichia coli Species Biodiversity Reveals New Biofilm-Associated Antiadhesion Polysaccharides

mBio ◽  
2011 ◽  
Vol 2 (3) ◽  
Author(s):  
Olaya Rendueles ◽  
Laetitia Travier ◽  
Patricia Latour-Lambert ◽  
Thierry Fontaine ◽  
Julie Magnus ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Adhesion ◽  
Bioactive Molecules ◽  
Bacterial Biofilms ◽  
Gram Positive ◽  
Content Type ◽  
E Coli ◽  
Species Biodiversity

ABSTRACTBacterial biofilms often form multispecies communities in which complex but ill-understood competition and cooperation interactions occur. In light of the profound physiological modifications associated with this lifestyle, we hypothesized that the biofilm environment might represent an untapped source of natural bioactive molecules interfering with bacterial adhesion or biofilm formation. We produced cell-free solutions extracted fromin vitromature biofilms formed by 122 naturalEscherichia coliisolates, and we screened these biofilm extracts for antiadhesion molecules active on a panel of Gram-positive and Gram-negative bacteria. Using this approach, we showed that 20% of the tested biofilm extracts contained molecules that antagonize bacterial growth or adhesion. We characterized a compound, produced by a commensal animalE. colistrain, for which activity is detected only in biofilm extract. Biochemical and genetic analyses showed that this compound corresponds to a new type of released high-molecular-weight polysaccharide whose biofilm-associated production is regulated by the RfaH protein. We demonstrated that the antiadhesion activity of this polysaccharide was restricted to Gram-positive bacteria and that its production reduced susceptibility to invasion and provided rapid exclusion ofStaphylococcus aureusfrom mixedE. coliandS. aureusbiofilms. Our results therefore demonstrate that biofilms contain molecules that contribute to the dynamics of mixed bacterial communities and that are not or only poorly detected in unconcentrated planktonic supernatants. Systematic identification of these compounds could lead to strategies that limit pathogen surface colonization and reduce the burden associated with the development of bacterial biofilms on medical devices.IMPORTANCEWe sought to demonstrate that bacterial biofilms are reservoirs for unknown molecules that antagonize bacterial adhesion. The use of natural strains representative ofEscherichia colispecies biodiversity showed that nonbiocidal antiadhesion polysaccharides are frequently found in mature biofilm extracts (bacterium-free suspensions which contain soluble molecules produced within the biofilm). Release of an antiadhesion polysaccharide confers a competitive advantage upon the producing strain against clinically relevant pathogens such asStaphylococcus aureus. Hence, exploring the biofilm environment provides a better understanding of bacterial interactions within complex communities and could lead to improved control of pathogen colonization.

scholarly journals A New Prenylated Flavanoid with Antibacterial Activity from Propolis Collected in Egypt

2010 ◽  
Vol 5 (1) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Ashraf El-Bassuony ◽  
Sameh AbouZid
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Spectroscopic Methods ◽  
Gram Positive ◽  
Gram Negative

A novel prenylated flavanoid, isonymphaeol-D (1), together with two known compounds, isonymphaeol-B (2) and nymphaeol-B (3), were isolated from Egyptian propolis. The structures of the isolated compounds were determined by various spectroscopic methods. 1 exhibited antibacterial activity against Gram-positive (Bacillus cereus, Staphylococcus aureus) and Gram-negative strains (Serratia sp., Pseudomonos sp., Escherichia coli).

scholarly journals Nitroxide Functionalized Antibiotics Are Promising Eradication Agents against Staphylococcus aureus Biofilms

2019 ◽  
Vol 64 (1) ◽  
Author(s):  
Anthony D. Verderosa ◽  
Rabeb Dhouib ◽  
Kathryn E. Fairfull-Smith ◽  
Makrina Totsika
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Biofilms ◽  
Gram Positive ◽  
Gram Negative ◽  
Content Type ◽  
Microscopy Analysis ◽  
Biofilm Dispersal ◽  
Biofilm Structure ◽  
Dispersal Activity

ABSTRACT Treatment of biofilm-related Staphylococcus aureus infections represents an important medical challenge worldwide, as biofilms, even those involving drug-susceptible S. aureus strains, are highly refractory to conventional antibiotic therapy. Nitroxides were recently shown to induce the dispersal of Gram-negative biofilms in vitro, but their action against Gram-positive bacterial biofilms remains unknown. Here, we demonstrate that the biofilm dispersal activity of nitroxides extends to S. aureus, a clinically important Gram-positive pathogen. Coadministration of the nitroxide CTEMPO (4-carboxy-2,2,6,6-tetramethylpiperidin-1-yloxyl) with ciprofloxacin significantly improved the biofilm eradication activity of the antibiotic against S. aureus. Moreover, covalently linking the nitroxide to the antibiotic moiety further reduced the ciprofloxacin minimal biofilm eradication concentration. Microscopy analysis revealed that fluorescent nitroxide-antibiotic hybrids could penetrate S. aureus biofilms and enter cells localized at the surface and base of the biofilm structure. No toxicity to human cells was observed for the nitroxide CTEMPO or the nitroxide-antibiotic hybrids. Taken together, our results show that nitroxides can mediate the dispersal of Gram-positive biofilms and that dual-acting biofilm eradication antibiotics may provide broad-spectrum therapies for the treatment of biofilm-related infections.

Antibacterial Activity of Amine-Functionalized Zeolite NaY against Staphylococcus aureus ATCC6538 and Escherichia coli ATCC11229

2015 ◽  
Vol 761 ◽  
pp. 402-406 ◽  
Author(s):  
Siti Aishah Mohd Hanim ◽  
Nik Ahmad Nizam Nik Malek ◽  
Zaharah Ibrahim ◽  
Mashitah Mad Salim ◽  
Nur Isti'anah Ramli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Gram Positive ◽  
Gram Negative ◽  
Zeolite Nay ◽  
Amine Functionalized ◽  
Diffusion Technique

The antibacterial activity of functionalized zeolite NaY (CBV100) with different concentrations of 3-aminopropyltriethoxysilane (APTES) (0.01, 0.05, 0.20 and 0.40 M) was studied against Staphylococcus aureus ATCC 6538 (Gram positive) and Escherichia coli ATCC 11229 (Gram negative) through disc diffusion technique (DDT). The characterization of functionalized zeolite NaY with fourier transform infrared (FTIR) spectroscopy indicated the attachment of APTES on zeolite NaY. Through DDT, the inhibition zone of functionalized zeolite NaY increased proportionally to the amount of the amine-functional group attached onto zeolite NaY. Functionalized zeolite NaY showed higher antibacterial activity against Gram-positive compared to Gram-negative bacteria. It can be concluded from this study that amine-functionalized zeolite NaY shows evidence of antibacterial activities.

Survival of some non-starter bacteria in naturally ripened and enzyme-accelerated Cheddar cheese

1988 ◽  
Vol 55 (4) ◽  
pp. 597-602 ◽  
Author(s):  
Lydia Bautista ◽  
Rohan G. Kroll
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Enterococcus Faecalis ◽  
Enzyme System ◽  
Cheddar Cheese ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Ripening Period ◽  
Gram Positive Bacteria

SummaryEffects of the addition of a proteinase (Neutrase 1–5S) and a peptidase (aminopeptidase DP-102) as agents for accelerating the ripening of Cheddar cheese on the survival of some non-starter bacteria (Staphylococcus aureus, Enterococcus faecalis, Escherichia coliand aSalmonellasp.) were studied throughout a 4-month ripening period. The enzymes were found to have no significant effect on the survival of the Gram-positive bacteria but some significant effects were observed, at some stages of the ripening period, with the Gram-negative bacteria in that lower levels were recovered from cheeses treated with the enzyme system.

scholarly journals Burdock (Arctium lappa) Leaf Extracts Increase the In Vitro Antimicrobial Efficacy of Common Antibiotics on Gram-positive and Gram-negative Bacteria

2017 ◽  
Vol 15 (1) ◽  
pp. 92-102 ◽  
Author(s):  
Lucia Pirvu ◽  
Isabela Nicorescu ◽  
Cristina Hlevca ◽  
Bujor Albu ◽  
Valentin Nicorescu
Keyword(s):  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Leaf Extracts ◽  
Gram Positive ◽  
Polyphenol Compounds ◽  
Arctium Lappa ◽  
Gram Negative ◽  
E Coli ◽  
Polar Extracts

AbstractThis work aimed to study the potential effects of four Arctii folium extracts, 5 mg gallic [GAE] acid equivalents per 1 mL sample, on six antibiotics (Ampicillin/AM, Tetracycline/TE, Ciprofloxacin/CIP, Sulfamethoxazole-Trimethoprim/SXT, Chloramphenicol/C and Gentamicin/CN) tested on four Gram-positive (Staphylococcus aureus ATCC 6538, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, and Staphylococcus epidermidis ATCC 12228) and five Gram-negative (Proteus mirabilis ATCC 29245, Escherichia coli ATCC 35218, E. coli ATCC 11229, E. coli ATCC 8739, and Bacillus cereus ATCC 11778) bacteria. Arctii folium extracts were the whole ethanol extract/W and subsequent ethyl acetate/EA, aqueous/AQ, and chloroform/CHL fractions. Chemical qualitative analysis (HPTLC method) emphasized five main polyphenol compounds in Arctii folium polar extracts: chlorogenic acid (Rf≈0.52/0.55) and its isomer, 1,5-di-O-caffeoylquinic acid (Rf≈0.90/0.92), plus cynarin (Rf≈0.77), hyperoside (Rf≈0.68/0.64) and isoquercitrin (Rf≈0.69/0.71). Microbiological screening indicated Arctii folium polar extracts (AQ and W) efficacy on S. epidermidis ATCC 12228; the MIC values were in the range of common antibiotics, being 32 and 128 μg GAE per mL sample respectively. The unpredictable effects (stimulatory or inhibitory) of Arctii folium extracts in combination with typical antibiotics as well as a potential use of the whole ethanol extract/W for restoring the antimicrobial potency of susceptible antibiotics have also been evidenced.

Lactoferrin-Thymol Complex for Disinfection of Gram-Positive Staphylococcus aureus and Gram-Negative Escherichia coli

2021 ◽  
Author(s):  
Mi Yan ◽  
Shuyue Dong ◽  
Xue Shen ◽  
Chengwen Lu ◽  
Haiqing Ye ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Food Industry ◽  
Antibacterial Agents ◽  
Environmentally Friendly ◽  
Gram Positive ◽  
Gram Negative ◽  
Human Safety

Seeking all-nature derived antibacterial agents with effective disinfection function, high human safety as well as environmentally-friendly characteristics is highly required in food industry. Herein, we report lactoferrin-thymol (LF-Thy) complex as...

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