Histidine-Lacked Aβ(1–16) Peptides: pH-Dependent Conformational Changes in Metal Ion Binding

Relative order of the pH-dependent conditional stability of the hydroxamate type (O,O) and (Namino,Nhydroxamato) chelates determines the coordination modes in the various mono- and dinuclear complexes of [(η5-Cp*)RhIII(H2O)3]2+ with α-, β- and γ-aminohydroxamic acids.

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Urea Gel Electrophoresis in Studies of Conformational Changes of Transferrin on Binding and Transport of Non-Ferric Metal Ions

Gels ◽

10.3390/gels8010019 ◽

2021 ◽

Vol 8 (1) ◽

pp. 19

Author(s):

Aviva Levina ◽

Boer Wang ◽

Peter A. Lay

Keyword(s):

Metal Ions ◽

Conformational Changes ◽

Gel Electrophoresis ◽

Metal Ion ◽

Polyacrylamide Gel Electrophoresis ◽

Ion Binding ◽

Metal Ion Binding ◽

Transporter Protein ◽

Systematic Exploration ◽

Acidic Conditions

Transferrin (Tf) is a crucial transporter protein for Fe(III), but its biological role in binding other metal ions and their delivery into cells remain highly controversial. The first systematic exploration of the effect of non-Fe(III) metal ion binding on Tf conformation has been performed by urea-polyacrylamide gel electrophoresis (urea-PAGE), which is commonly used for nucleic acids but rarely for proteins. Closed Tf conformation, similar to that caused by Fe(III)-Tf binding, was formed for In(III), V(III) or Cr(III) binding to Tf. In all these cases, metal distribution between Tf lobes and/or the rate of metal release under acidic conditions differed from that of Fe(III)-Tf. By contrast, Ga(III) and V(IV) did not form closed Tf conformation under urea-PAGE conditions. Apart from Fe(III), only In(III) was able to increase the proportion of closed Tf conformation in whole serum. These results suggest that Tf is unlikely to act as a natural carrier of any metal ion, except Fe(III), into cells but can reduce toxicity of exogenous metal ions by binding them in serum and preventing their entry into cells.

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Faculty Opinions recommendation of Fusogenic supramolecular vesicle systems induced by metal ion binding to amphiphilic ligands.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1021994.251625 ◽

2004 ◽

Author(s):

Bradley Smith

Keyword(s):

Metal Ion ◽

Ion Binding ◽

Metal Ion Binding

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Photophysical Characterisation, Metal Ion Binding and Enantiomeric Recognition of Chiral Ligands Containing Phenazine Fluorophore

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc20040885 ◽

2004 ◽

Vol 69 (4) ◽

pp. 885-896 ◽

Cited By ~ 8

Author(s):

Luisa Stella Dolci ◽

Péter Huszthy ◽

Erika Samu ◽

Marco Montalti ◽

Luca Prodi ◽

...

Keyword(s):

Metal Ion ◽

Photophysical Properties ◽

Ion Binding ◽

Metal Ion Binding ◽

Ammonium Ions ◽

Enantiomerically Pure ◽

Binding Process ◽

High Affinity ◽

Enantiomeric Recognition ◽

Chiral Species

Enantiomerically pure dimethyl- and diisobutyl-substituted phenazino-18-crown-6 ligands bind metal and ammonium ions and also primary aralkylammonium perchlorates in acetonitrile with high affinity, causing pronounced changes in their luminescence properties. In addition, they show enantioselectivity towards chiral primary aralkylammonium perchlorates. The possibility to monitor the binding process by photoluminescence spectroscopy can gain ground for the design of very efficient enantioselective chemosensors for chiral species. The observed changes in the photophysical properties are also an important tool for understanding the interactions present in the adduct.

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