De novo characterization of the antler tip of Chinese Sika deer transcriptome and analysis of gene expression related to rapid growth

2011 ◽  
Vol 364 (1-2) ◽  
pp. 93-100 ◽  
Author(s):  
Baojin Yao ◽  
Yu Zhao ◽  
Qun Wang ◽  
Mei Zhang ◽  
Meichen Liu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rapid Growth ◽  
Sika Deer ◽  
De Novo ◽  
Chinese Sika Deer ◽  
Antler Tip

Sequencing and de novo analysis of the Chinese Sika deer antler-tip transcriptome during the ossification stage using Illumina RNA-Seq technology

2012 ◽  
Vol 34 (5) ◽  
pp. 813-822 ◽  
Author(s):  
Baojin Yao ◽  
Yu Zhao ◽  
Haishan Zhang ◽  
Mei Zhang ◽  
Meichen Liu ◽  
...  
Keyword(s):  
Sika Deer ◽  
De Novo ◽  
Rna Seq ◽  
Deer Antler ◽  
Chinese Sika Deer ◽  
Antler Tip

scholarly journals Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Magda Grudniewska ◽  
Stijn Mouton ◽  
Daniil Simanov ◽  
Frank Beltman ◽  
Margriet Grelling ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
De Novo ◽  
Transcriptome Assembly ◽  
Model Organism ◽  
Proliferating Cells ◽  
Macrostomum Lignano ◽  
Germline Cells

The regeneration-capable flatworm Macrostomum lignano is a powerful model organism to study the biology of stem cells in vivo. As a flatworm amenable to transgenesis, it complements the historically used planarian flatworm models, such as Schmidtea mediterranea. However, information on the transcriptome and markers of stem cells in M. lignano is limited. We generated a de novo transcriptome assembly and performed the first comprehensive characterization of gene expression in the proliferating cells of M. lignano, represented by somatic stem cells, called neoblasts, and germline cells. Knockdown of a selected set of neoblast genes, including Mlig-ddx39, Mlig-rrm1, Mlig-rpa3, Mlig-cdk1, and Mlig-h2a, confirmed their crucial role for the functionality of somatic neoblasts during homeostasis and regeneration. The generated M. lignano transcriptome assembly and gene expression signatures of somatic neoblasts and germline cells will be a valuable resource for future molecular studies in M. lignano.

scholarly journals De Novo Characterization of Japanese Scallop Mizuhopecten yessoensis Transcriptome and Analysis of Its Gene Expression following Cadmium Exposure

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e64485 ◽  
Author(s):  
Xiao-lin Meng ◽  
Mei Liu ◽  
Ke-yong Jiang ◽  
Bao-jie Wang ◽  
Xue Tian ◽  
...  
Keyword(s):  
Gene Expression ◽  
De Novo ◽  
Cadmium Exposure ◽  
Japanese Scallop ◽  
Mizuhopecten Yessoensis ◽  
Its Gene

scholarly journals Differential Gene Expression Between Polymorphic Zooids of the Marine Bryozoan Bugulina stolonifera

2020 ◽  
Vol 10 (10) ◽  
pp. 3843-3857
Author(s):  
Kira A. Treibergs ◽  
Gonzalo Giribet
Keyword(s):  
Gene Expression ◽  
Division Of Labor ◽  
Differential Gene Expression ◽  
High Throughput Sequencing ◽  
De Novo ◽  
Transcriptome Assembly ◽  
Cdna Libraries ◽  
Living Species ◽  
Differential Gene

Bryozoans are a diverse phylum of marine and freshwater colonial invertebrates containing approximately 6,300 described living species. Bryozoans grow by budding new physiologically connected colony members (zooids) from a founding individual that forms from a metamorphosed larva. In some species these zooids come in different shapes and sizes and are specialized to serve different tasks within the colony. A complex interaction of genotype, environment, and developmental pathway shapes zooid fate, however, the specific mechanisms underlying the establishment of this division of labor remain unknown. Here, the first characterization of differential gene expression between polymorphic zooids of a bryozoan colony is presented. The development of different zooid types of lab-cultured Bugulina stolonifera colonies including feeding autozooids, avicularia (derived non-feeding zooids that are homologous to feeding autozooids but shaped like a bird’s beak), and rhizoids (a branching network of non-feeding anchoring zooids) was explored using RNA sequencing, de novo transcriptome assembly, and differential gene expression analyses. High throughput sequencing of cDNA libraries yielded an average of 14.9 ± 1.3 (SE) million high-quality paired-end reads per sample. Data for the first de novo transcriptome assemblies of B. stolonifera and the first characterization of genes involved in the formation and maintenance of zooid types within a bryozoan colony are presented. In a comparison between autozooid and avicularium tissues, 1,097 significant differentially expressed genes were uncovered. This work provides a much-needed foundation for understanding the mechanisms involved in the development of polymorphic zooids and the establishment of division of labor in bryozoans.

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