Accumulation of quantitative trait loci conferring broad-spectrum clubroot resistance in Brassica oleracea

2013 ◽  
Vol 32 (4) ◽  
pp. 889-900 ◽  
Author(s):  
Hiroya Tomita ◽  
Motoki Shimizu ◽  
Md. Asad-ud Doullah ◽  
Ryo Fujimoto ◽  
Keiichi Okazaki
Keyword(s):  
Quantitative Trait Loci ◽  
Brassica Oleracea ◽  
Broad Spectrum ◽  
Quantitative Trait ◽  
Clubroot Resistance ◽  
Trait Loci

scholarly journals Identification of Candidate Genes for Clubroot-Resistance in Brassica oleracea Using Quantitative Trait Loci-Sequencing

2021 ◽  
Vol 12 ◽  
Author(s):  
Fuquan Ce ◽  
Jiaqin Mei ◽  
Haiyan He ◽  
Yu Zhao ◽  
Wenhui Hu ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Brassica Oleracea ◽  
Candidate Genes ◽  
Quantitative Trait ◽  
Plasmodiophora Brassicae ◽  
Potential Candidate ◽  
Clubroot Resistance ◽  
Group I ◽  
Trait Loci ◽  
Group Ii

Clubroot caused by Plasmodiophora brassicae is a devastating disease of cabbage (Brassica oleracea). To identify quantitative trait loci (QTLs) for clubroot resistance (CR) in B. oleracea, genomic resequencing was carried out in two sets of extreme pools, group I and group II, which were constructed separately from 110 and 74 F2 cloned lines derived from the cross between clubroot-resistant (R) cabbage “GZ87” (against race 4) and susceptible (S) cabbage “263.” Based on the QTL-sequencing (QTL-Seq) analysis of group I and group II, three QTLs (i.e., qCRc7-2, qCRc7-3, and qCRc7-4) were determined on the C07 chromosome. RNA-Seq and qRT-PCR were conducted in the extreme pools of group II before and after inoculation, and two potential candidate genes (i.e., Bol037115 and Bol042270), which exhibiting upregulation after inoculation in the R pool but downregulation in the S pool, were identified from the three QTLs on C07. A functional marker “SWU-OA” was developed from qCRc7-4 on C07, exhibiting ∼95% accuracy in identifying CR in 56 F2 lines. Our study will provide valuable information on resistance genes against P. brassicae and may accelerate the breeding process of B. oleracea with CR.

Quantitative trait loci affecting plant regeneration from protoplasts of Brassica oleracea

2004 ◽  
Vol 108 (8) ◽  
pp. 1513-1520 ◽  
Author(s):  
I. B. Holme ◽  
A. M. Torp ◽  
L. N. Hansen ◽  
S. B. Andersen
Keyword(s):  
Quantitative Trait Loci ◽  
Plant Regeneration ◽  
Brassica Oleracea ◽  
Quantitative Trait ◽  
Trait Loci

scholarly journals Polygenic Resistance of Pepper to Potyviruses Consists of a Combination of Isolate-Specific and Broad-Spectrum Quantitative Trait Loci

1997 ◽  
Vol 10 (7) ◽  
pp. 872-878 ◽  
Author(s):  
Carole Caranta ◽  
Véronique Lefebvre ◽  
Alain Palloix
Keyword(s):  
Quantitative Trait Loci ◽  
Broad Spectrum ◽  
Quantitative Trait ◽  
Quantitative Resistance ◽  
Specific Effect ◽  
Major Effect ◽  
Minor Effect ◽  
Resistance Factors ◽  
Specific Effects ◽  
Trait Loci

At least six potyviruses infect pepper crops around the world. Only monogenic resistance with isolate-specific effects or broad-spectrum effects against potyviruses has been identified. Quantitative trait loci (QTLs) for resistance to two potato virus Y (PVY) isolates and to potyvirus E have been mapped in a doubled-haploid progeny from the hybrid between the line Perennial with resistance factors against several potyviruses and the line Yolo Wonder, with 172 DNA markers. Eleven chromosomal regions were found to be associated with quantitative resistance to PVY and potyvirus E by both analysis of variance and non-parametric tests. This resistance results from the association of a major-effect QTL and several minor-effect QTLs. Two minor-effect QTLs originated from the susceptible parent. In addition to additive-effect QTLs, interactions between QTLs were also identified. Based on their spectrum of action, we discriminated QTLs with PVY isolate-or potyvirus-specific effect and loci involved in the resistance to several distinct potyviruses. The locations of QTLs were compared with loci having known qualitative effects against potyviruses; QTLs for PVY and potyvirus E resistance were detected in the vicinity of the pvr2 and pvr6 loci. The origin of the broad-spectrum resistance against potyviruses and the relationships between major genes and QTLs are discussed.

Comparative Mapping of Quantitative Trait Loci Sculpting the Curd of Brassica oleracea

Genetics ◽  
2000 ◽  
Vol 155 (4) ◽  
pp. 1927-1954 ◽  
Author(s):  
Tien-Hung Lan ◽  
Andrew H Paterson
Keyword(s):  
Quantitative Trait Loci ◽  
Brassica Oleracea ◽  
Quantitative Trait ◽  
Genetic Basis ◽  
Allelic Variation ◽  
Morphological Data ◽  
Human Consumption ◽  
Trait Loci ◽  
Challenges And Opportunities ◽  
Different Populations

Abstract The enlarged inflorescence (curd) of cauliflower and broccoli provide not only a popular vegetable for human consumption, but also a unique opportunity for scientists who seek to understand the genetic basis of plant growth and development. By the comparison of quantitative trait loci (QTL) maps constructed from three different F2 populations, we identified a total of 86 QTL that control eight curd-related traits in Brassica oleracea. The 86 QTL may reflect allelic variation in as few as 67 different genetic loci and 54 ancestral genes. Although the locations of QTL affecting a trait occasionally corresponded between different populations or between different homeologous Brassica chromosomes, our data supported other molecular and morphological data in suggesting that the Brassica genus is rapidly evolving. Comparative data enabled us to identify a number of candidate genes from Arabidopsis that warrant further investigation to determine if some of them might account for Brassica QTL. The Arabidopsis/Brassica system is an important example of both the challenges and opportunities associated with extrapolation of genomic information from facile models to large-genome taxa including major crops.

Identification of quantitative trait loci controlling partial clubroot resistance in new mapping populations of Arabidopsis thaliana

2008 ◽  
Vol 117 (2) ◽  
pp. 191-202 ◽  
Author(s):  
Mélanie Jubault ◽  
Christine Lariagon ◽  
Matthieu Simon ◽  
Régine Delourme ◽  
Maria J. Manzanares-Dauleux
Keyword(s):  
Arabidopsis Thaliana ◽  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Clubroot Resistance ◽  
Trait Loci ◽  
Mapping Populations
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