Association analysis, genetic diversity and structure analysis of tobacco based on AFLP markers

2014 ◽  
Vol 41 (5) ◽  
pp. 3317-3329 ◽  
Author(s):  
Ahmad Reza Dadras ◽  
Hossein Sabouri ◽  
Ghasem Mohammadi Nejad ◽  
Atefeh Sabouri ◽  
Mardavij Shoai-Deylami
2021 ◽  
Author(s):  
Varun Hiremath ◽  
Kanwar Pal Singh ◽  
Neelu Jain ◽  
Kishan Swaroop ◽  
Pradeep Kumar Jain ◽  
...  

Abstract Genetic diversity and structure analysis using molecular markers is necessary for efficient utilization and sustainable management of gladiolus germplasm. Genetic analysis of gladiolus germplasm using SSR markers is largely missing due to scarce genomic information. In the present investigation, we report 66.66% cross transferability of Gladiolus palustris SSRs whereas 48% of Iris EST-SSRs were cross transferable across the gladiolus genotypes used in the study. A total of 17 highly polymorphic SSRs revealed a total 58 polymorphic loci ranging from two to six in each locus with an average of 3.41 alleles per marker. PIC values ranged from 0.11 to 0.71 with an average value of 0.48. Four SSRs were selectively neutral based on Ewens-Watterson test. Analysis of genetic structure of 84 gladiolus genotypes divided whole germplasm into two subpopulations. 35 genotypes were assigned to subpopulation 1 whereas 37 to subpopulation 2 and rest of the genotypes recorded as admixture. Analysis of molecular variance indicated maximum variance (53.59%) among individuals within subpopulations whereas 36.55% of variation observed among individuals within total population. Least variation (9.86%) was noticed between two subpopulations. Moderate (FST = 0.10) genetic differentiation of two subpopulations was observed. Grouping pattern of population structure was consistent with UPGMA dendrogram based on simple matching dissimilarity coefficient (ranged from 01.6 to 0.89) and PCoA. Genetic relationships assessed among the genotypes of respective clusters assist the breeders in selecting desirable parents for crossing. SSR markers from present study can be utilized for cultivar identification, conservation and sustainable utilization of gladiolus genotypes for crop improvement.


2014 ◽  
Vol 89 (6) ◽  
pp. 707-711 ◽  
Author(s):  
SH. Kolahi-Zonoozi ◽  
M. Mardi ◽  
M. Zeinalabedini ◽  
S. M. Pirseyedi ◽  
P. Mahmoodi ◽  
...  

2020 ◽  
Vol 26 (11) ◽  
pp. 2255-2266
Author(s):  
Rahul Kumar ◽  
Sumeet Parkash Kaundal ◽  
Vikas Sharma ◽  
Ashutosh Sharma ◽  
Gagandeep Singh ◽  
...  

2016 ◽  
Vol 2 (2) ◽  
pp. 15-32 ◽  
Author(s):  
Fatemeh Sahranavard Azartamar ◽  
Mortaza Ghadimzadeh ◽  
Reza Darvishzadeh ◽  
◽  
◽  
...  

HortScience ◽  
2014 ◽  
Vol 49 (5) ◽  
pp. 531-537 ◽  
Author(s):  
Sara Melito ◽  
Angela Fadda ◽  
Emma Rapposelli ◽  
Maurizio Mulas

Myrtle is an aromatic plant typical of the Mediterranean maquis. It is widely exploited in pharmaceutical, cosmetic, and food industries, whereas in Italy, it is mainly used for the production of the typical liqueur. Amplified fragment length polymorphism (AFLP) markers were used to evaluate the genetic variability of some Sardinian Myrtus communis L. candidate cultivars. The AFLP selective amplification produced 138 reproducible AFLP fragments, 96% of which were polymorphic. STRUCTURE analysis divided the myrtle accessions into two main genetic groups (K = 2). The two clusters showed different numbers of individuals. Most of the individuals belonged to Cluster B, whereas only eight genotypes were attributed to Cluster A. Unweighted pair group method with Arithmetic Mean (UPGMA) dendrogram segregated all the myrtle cultivars into five main groups, displaying a partial congruence with the division observed by STRUCTURE analysis. The analysis of the genetic diversity distribution in a candidate cultivar selection displayed a geographical gradient of myrtle from north to south, which reflects the Sardinian shape, and from west to east, which reflects the Sardinian mountain distribution.


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