scholarly journals Assessment of Genetic Diversity and Structure in the Wild Melon (Cucumis melo var. agrestis) Genotypes from Southern Coastline of Caspian Sea using AFLP Markers

2017 ◽  
Vol 9 (21) ◽  
pp. 67-75
Author(s):  
Fatemeh Vafadar Sham Asbi ◽  
Ali Dehestani ◽  
Saber Golkari ◽  
Mosafa Hagh Panah
2014 ◽  
Vol 41 (5) ◽  
pp. 3317-3329 ◽  
Author(s):  
Ahmad Reza Dadras ◽  
Hossein Sabouri ◽  
Ghasem Mohammadi Nejad ◽  
Atefeh Sabouri ◽  
Mardavij Shoai-Deylami

HortScience ◽  
2003 ◽  
Vol 38 (2) ◽  
pp. 256-259 ◽  
Author(s):  
Margaret R. Pooler

Many popular crapemyrtle (Lagerstroemia L.) cultivars grown in the United States are interspecific hybrids between L. indica L. and L. fauriei Koehne. The 22 hybrid cultivars released from the U.S. National Arboretum contain primarily genetic material from L. fauriei PI 237884. Examining the genetic diversity ofL. fauriei specimens in the U.S. is valuable because of the historical and economic significance of the species, the increasing interest it is receiving as a source of new cultivars, and its threatened status in the wild. Our objectives were to examine molecular genetic diversity among L. fauriei accessions using Randomly Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphisms (AFLP) markers. Our results indicate: 1) RAPD and AFLP markers are generally consistent in the genetic relationships that they suggest; 2) the L. fauriei germplasm we examined falls into at least three distinct clusters; and 3) the genetic base of cultivated Lagerstroemia could be broadened significantly by incorporating some of this more diverse L. fauriei germplasm into breeding programs.


Crustaceana ◽  
2015 ◽  
Vol 88 (1) ◽  
pp. 1-17
Author(s):  
Xiaoying Li ◽  
Zhiguo Dong ◽  
Hongbo Su ◽  
Qingqi Zhang ◽  
Huan Gao ◽  
...  

Using fluorescent AFLP-markers, the genetic diversity and structure of six geographical populations of the swimming crab,Portunus trituberculatus(Miers, 1876), from the coast of China were analysed to assess their genetic resource state. Eight primer combinations generated 925 loci among 85 individuals, and revealed a high polymorphism within these populations that varied from 57.41% (Zhoushan population) to 76.86% (Dalian population). According to the combination of percentage of polymorphic loci (PPL) and Shannon’s information index (I), the populations from Zhangzhou, Dongying and Dalian showed more genetic variation than the Lianyungang, Zhanjiang and Zhoushan populations. The gene flow () and across all populations overall were 1.935 and 0.205, respectively, showing moderate genetic differentiation. Differentiation was highest in the Zhoushan and Zhanjiang populations, and the geographical distribution and UPGMA cluster tree were not fully accordant. Neutrality test results revealed that the Zhangzhou population faced a smaller selective pressure than the other five populations (). In general, a high genetic diversity among theP. trituberculatuspopulations was observed along the coast of China, especially in the Zhangzhou, Dongying, Dalian and Lianyungang populations, while a pronounced level () of genetic differentiation has occurred between the Zhoushan population and the other populations. Therefore, these findings have the following implications for conservation and genetic improvement: (1) high diversity levels and good genetic resources, especially in the Zhangzhou population, which could lay the foundations for breeding research and genetic improvement; (2) the importance of preventing species escape and gene pool contamination in ex-situ conservation and species introduction programs in the Zhoushan population.


Hereditas ◽  
2019 ◽  
Vol 156 (1) ◽  
Author(s):  
Wen-Dan Wu ◽  
Wen-Hui Liu ◽  
Ming Sun ◽  
Ji-Qiong Zhou ◽  
Wei Liu ◽  
...  

2019 ◽  
Vol 12 ◽  
pp. 194008291984950 ◽  
Author(s):  
Yayan Wahyu C. Kusuma ◽  
Siti R. Ariati ◽  
Rosniati A. Risna ◽  
Chika Mitsuyuki ◽  
Yoshihisa Suyama ◽  
...  

Ex situ conservation is an important complementary strategy for in situ to conserve endangered plant species. However, the limited areas designated for ex situ conservation such as in botanic gardens have become a great challenge for conservation practitioners and scientists attempting to optimally conserve the genetic diversity of targeted plant species. Our study aimed to assess genetic diversity and structure of wild seedlings of Vatica bantamensis, an endemic and critically endangered dipterocarp from Java (Indonesia). We also estimated genetic differentiation between the wild seedlings and existing ex situ collection and evaluated the genetic diversity preserved in the ex situ collection. Our analysis, using 730 single-nucleotide polymorphisms loci, showed that wild seedlings exhibited higher genetic diversity than the ex situ collection (nucleotide diversity, µ = 0.26 and 0.16, respectively). Significant genetic differentiation was also detected ( FST = 0.32) between wild seedlings and ex situ collection. Furthermore, we found high kinship within the ex situ collection suggesting low genetic diversity since the founding collection. We also detected three distinct genetic clusters from all samples combined (analysis of molecular variance, ϕ = 0.48, p < .001), with two clusters present in the wild seedlings that were not represented in the ex situ collection. We recommend that supplementary collections from the two newly identified genetic clusters in the wild seedlings should be incorporated to increase genetic diversity in the ex situ collection. Furthermore, our study demonstrated that understanding the population genetics of targeted endangered species provides better results for ex situ conservation strategies.


Genetica ◽  
2016 ◽  
Vol 144 (6) ◽  
pp. 627-638 ◽  
Author(s):  
Eleonora Zambrano Blanco ◽  
Miklos Maximiliano Bajay ◽  
Marcos Vinícius Bohrer Monteiro Siqueira ◽  
Maria Imaculada Zucchi ◽  
José Baldin Pinheiro

2015 ◽  
Vol 43 (2) ◽  
pp. 528-535 ◽  
Author(s):  
Jamila MOUHADDAB ◽  
Naima AIT AABD ◽  
Hafid ACHTAK ◽  
Fouad MSANDA ◽  
Abdelaziz ZAHIDI ◽  
...  

The preservation of the diversity of endangered populations of argan trees, in their natural habitat, is a crucial step toward their conservation. The aim of the present study was to evaluate the genetic diversity of the argan trees in the wild, and to establish a phylogenetic map using DNA fingerprints. The ultimate goal was to develop a core set that would represent the existing diversity in the whole germplasm. In regard to this, 200 samples of Argania spinosa individual trees were collected from 10 different provenances in the region of Essaouira (Morocco). The genetic variation between and within these argan trees was investigated using previously described Inter-Simple Sequence Repeat markers. These markers generated a total of 149 fragments, in which 148 (99.33%) were polymorphic. The samples collected in the ‘Ouled Lhaj’ provenance showed the lowest diversity (% of polymorphic locus P=48.32%; genetic diversity Nei h=0.153; allelic richness A=1.483), compared to those collected in the ‘Mramer’ provenance (%P=68.46%; h=0.233; A=1.685). Also, the results showed a high level of genetic differentiation among provenances (AMOVA=44%, Gst=0.40), and a limited gene flow (Nm=0.73) between the provenances. In addition, these data suggested a low correlation between the genetic diversity of the tree and their respective geographical location in relation to the proximity to the littoral. Finally, a core collection of 13 genotypes that represent the essential of the detected diversity was established. The distribution pattern of this genetic diversity provides an important baseline data for the conservation strategies of argan tree species in the wild.


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