Genetic diversity in Corchorus olitorius L. revealed by morphophysiological and molecular analyses

2019 ◽  
Vol 46 (3) ◽  
pp. 2933-2940 ◽  
Author(s):  
Ahmed Fathy Youssef ◽  
Nabil Ahmed Younes ◽  
Muhammad Youssef
Keyword(s):  
Genetic Diversity ◽  
Corchorus Olitorius ◽  
Molecular Analyses

scholarly journals The Associations ofLeishmania majorandLeishmania tropicaAspects by Focusing Their Morphological and Molecular Features on Clinical Appearances in Khuzestan Province, Iran

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Adel Spotin ◽  
Soheila Rouhani ◽  
Parviz Parvizi
Keyword(s):  
Genetic Diversity ◽  
Its Rdna ◽  
Molecular Analyses ◽  
Khuzestan Province ◽  
Molecular Features ◽  
Low Genetic Diversity ◽  
Irregular Shapes ◽  
Ocular Micrometer ◽  
Reservoir Hosts ◽  
Genetic Characters

Cutaneous leishmaniasis has various phenotypic aspects consisting of polymorphic amastigotes with different genetic ranges. Samples were collected from suspected patients of Khuzestan province. Prepared smears were stained, scaled, and measured using ocular micrometer. The Cytb, ITS-rDNA, and microsatellite genes ofLeishmaniawere amplified andLeishmaniaspecies were identified by molecular analyses. Of 150 examined suspected patients, 102 were identified toLeishmaniaspecies (90L. major, nineL. tropica, and three unidentified). The amastigotes of 90L. majorhad regular and different irregular shapes within three clinical lesions with no and/or low genetic diversity. Three haplotypes of CytbofL. majorwere found but no variation was observed using ITS-rDNA gene. Interesting findings were that all nineL. tropicahad regular amastigote shapes with more genetic variations, also a patient which had coinfection ofL. major,L. tropica, andCrithidia.At least twoL. majorandL. tropicawere identified in suspected patients of the regions. Different irregular amastigotes’ shapes ofL. majorcan be explained by various reservoir hosts and vectors. In contrast, more molecular variations inL. tropicacould be justified by genetic characters. UnidentifiedLeishmaniacould be mixed pathogens or nonpathogens with mammals’LeishmaniaorCrithidia.

scholarly journals Does the genetic diversity among pubescent white oaks in southern Italy, Sicily and Sardinia islands support the current taxonomic classification?

2020 ◽  
Author(s):  
Romeo Di Pietro ◽  
Antonio Luca Conte ◽  
Piera Di Marzio ◽  
Paola Fortini ◽  
Emmanuele Farris ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Expressed Sequence Tag ◽  
Taxonomic Classification ◽  
Genetic Assignment ◽  
Molecular Analyses ◽  
Genetic Groups ◽  
Ecological Features ◽  
Weak Separation ◽  
Expressed Sequence ◽  
Simple Sequence

AbstractMolecular diversity analysis of deciduous pubescent oaks was conducted for populations from Calabria, Sicily and Sardinia. The aims of this study were twofold. First, to provide data on the genetic diversity of pubescent oaks from an understudied area which currently exhibits one of the highest concentrations of pubescent oak species in Europe. Second, to verify if these groups of oaks are genetically distinct and if their identification is in accordance with the current taxonomic classification. Molecular analyses of leaf material of 480 trees from seventeen populations belonging to putatively different pubescent oak species (Quercus amplifolia, Q. congesta, Q. dalechampii, Q. ichnusae, Q. leptobalanos, Q. virgiliana) were performed. Twelve gene-based Expressed Sequence Tag-Simple Sequence Repeat markers were selected, and genetic diversity and differentiation were calculated. The results showed relatively high values of allelic richness, heterozygosity and number of private alleles for the populations investigated. A weak but positive correlation between geographical and genetic distance was detected. Genetic assignment (STRUCTURE) and principle coordinate analyses exhibited a weak separation into two genetic groups which, however, did not correspond to the taxonomic, chorological and ecological features of the populations investigated. Sardinian populations formed one group which was separated from the Calabrian and Sicilian populations. In light of the results obtained, the taxonomic classification for the pubescent white oaks currently reported in the major Italian floras and checklists for the study area was not confirmed by molecular analyses.

Investigation of IRAP transposon-based molecular markers for analysis of genetic diversity in pea germplasm

2018 ◽  
Author(s):  
Sajjad Ahmad ◽  
Rajvinder Kaur ◽  
Mark Lefsrud ◽  
Jaswinder Singh
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Dna Polymorphism ◽  
Breeding Programs ◽  
Molecular Analyses ◽  
Marker System ◽  
Varietal Identification ◽  
D Values ◽  
Mean Similarity ◽  
Germplasm Accessions

Retrotransposons diversity has been extensively studied in monocots, but it is not well documented in dicot species. Transposition activity of transposons creates DNA polymorphism and their abundant presence in genomes is making transposons a promising marker system for varietal identification and fingerprinting. In this study, four transposon-based markers (two DNA- and two RNA-transposons) were employed to evaluate the effectiveness of Inter-Retrotransposon Amplified Polymorphism (IRAP) transposon system in assessing genetic diversity in pea germplasm accessions. A total of 28 alleles were detected across the 35 pea accessions with number of alleles per locus ranged from 5 (Mutator) to 9 (Cyclops). RNA transposons produced a higher number of polymorphic alleles (Ogre: 8, Cyclops: 9) than DNA transposon markers (Mutator: 5, MITE: 6). Overall mean PIC value and D values for these transposon markers were 0.810 and 0.817 respectively. Genetic similarity values ranged from 0.143 to 0.823 with a mean similarity value of 0.403. Cluster analysis classified pea genotypes into six major groups that were somewhat consistent with their geographical origins. The molecular analyses differentiated all the 35 accessions and generated higher PIC and D values that can be useful for MAS-based breeding programs in pea.

scholarly journals The diversity of the Baikal lineage of Hydra oligactis Pallas, 1766: molecular and morphological evidence

ZooKeys ◽  
2020 ◽  
Vol 912 ◽  
pp. 1-12
Author(s):  
Tatiana E. Peretolchina ◽  
Igor V. Khanaev ◽  
Ilya V. Enushchenko ◽  
Dmitry Y. Sherbakov ◽  
Lyubov S. Kravtsova
Keyword(s):  
Genetic Diversity ◽  
Environmental Conditions ◽  
Morphological Analysis ◽  
Genetic Distances ◽  
Morphological Evidence ◽  
Molecular Analyses ◽  
Low Genetic Diversity ◽  
Morphological Approach

In this paper, molecular analyses of Baikal hydras from the ‘oligactis group’, based on COI and ITS1–5.8S–ITS2, and morphological analysis of their holotrichous isorhizas, were performed. Low genetic diversity and shared haplotypes were found between Hydra oligactis Pallas, 1766 and Hydra baikalensis Swarczewsky, 1923 specimens, which is evidence of the mixing of these lineages. Genetic distances among all Baikal hydras (0.006) were less than the interspecific distances of other hydras. The size of hydras and proportions of their holotrichous isorhizas varied depending on microhabitat and environmental conditions. Our combined molecular and morphological approach proves that H. baikalensis is synonymous with H. oligactis

scholarly journals Assessment of genetic diversity in Jute (Corchorus olitorius L.) under rain fed condition

2016 ◽  
Vol 7 (2) ◽  
pp. 454
Author(s):  
Sonika Yumnam ◽  
Ashutosh Sawarkar ◽  
S. Mukherjee ◽  
K. K. Sarkar
Keyword(s):  
Genetic Diversity ◽  
Corchorus Olitorius

scholarly journals Genetic diversity and relationships in jute (Corchorus spp.) revealed by SSR markers

1970 ◽  
Vol 38 (2) ◽  
pp. 153-161 ◽  
Author(s):  
Saaimatul Huq ◽  
Md Shahidul Islam ◽  
Abu Ashraqur Sajib ◽  
Nadim Ashraf ◽  
Samiul Haque ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Marker ◽  
Group Method ◽  
Individual Plant ◽  
Corchorus Olitorius ◽  
Corchorus Capsularis ◽  
Pair Group ◽  
Ssr Primers ◽  
Upgma Cluster Analysis

Characterization of sixteen jute genotypes, from Corchorus olitorius L. and Corchorus capsularis L. using jute specific SSR marker attained a high polymorphism value of 92.20%. A total of 171 different alleles were amplified by 27 primer pairs with a mean of 6.33 ± 2.04 alleles per locus. The genetic diversity was also relatively high (0.81 ± 0.06). The Un-weighted Pair-group Method with Arithmetic averages (UPGMA) cluster analysis of the 16 jute genotypes produced a dendogram, which was in concordance with known information. The study reinforces the utility of SSR primers for providing useful and high levels of markers for individual plant genotypes even with a narrow genetic base. Key words: Jute; Genetic diversity; SSR; Genotypes; Polymorphism DOI: 10.3329/bjb.v38i2.5140 Bangladesh J. Bot. 38(2): 153-161, 2009 (December)  

Disparity between morphology and genetics in Urtica dioica (Urticaceae)

2020 ◽  
Author(s):  
Ludmila Rejlová ◽  
Alžběta Böhmová ◽  
Zuzana Chumová ◽  
Šárka Hořčicová ◽  
Jiřina Josefiová ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Morphological Variation ◽  
Evolutionary History ◽  
Molecular Data ◽  
Urtica Dioica ◽  
Southwest Asia ◽  
Molecular Analyses ◽  
Evolutionary Force ◽  
Morphological And Molecular Data ◽  
Molecular Evaluation

Abstract Polyploidization is generally considered a major evolutionary force that can alter the genetic diversity, morphology, physiology and ecology of plants. One striking example is the polyploid Urtica dioica complex, in which diploid taxa are often found in remote and partly relictual geographical ranges, in contrast to tetraploid individuals, which have an unknown evolutionary history and occur in a variety of synanthropic habitats. We used a set of 279 plants, evenly representing the geographical and morphological variation of U. dioica s.l. in Europe and Southwest Asia, and employed multivariate and geometric morphometrics and Hyb-Seq sequencing to estimate the extent of differentiation of diploid taxa and the ubiquitous tetraploid cytotype. Diploid subspecies form more-or-less separate clusters in morphological analyses, but our molecular evaluation did not reveal any structure. Moreover, tetraploids coalesced with diploids in both morphological and molecular analyses. This disparity between morphological and molecular data might be driven by (1) local adaptation of the diploid cytotype that is mirrored in specific phenotypes, (2) only recent genetic diversification of the group and (3) homoploid and heteroploid hybridization events.

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