The synergistic anti-proliferative effect of the combination of diosmin and BEZ-235 (dactolisib) on the HCT-116 colorectal cancer cell line occurs through inhibition of the PI3K/Akt/mTOR/NF-κB axis

2020 ◽  
Vol 47 (3) ◽  
pp. 2217-2230 ◽  
Author(s):  
Maged. W. Helmy ◽  
Asser I. Ghoneim ◽  
Mohamed A. Katary ◽  
Rana K. Elmahdy
Keyword(s):  
Colorectal Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Line ◽  
Proliferative Effect ◽  
Hct 116

scholarly journals Anti-cancer potential of rhenium carbonyl complexes containing pyrrole ligands on colorectal cancer cell line HCT 116

2021 ◽  
Author(s):  
Veeranna Yempally ◽  
Queenie Fernandez ◽  
Lobna Safwan Al_Zaidan ◽  
Varghese Inchakalody ◽  
Maysaloun Merhi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Line ◽  
Carbonyl Complexes ◽  
Anti Cancer ◽  
Rhenium Carbonyl ◽  
Hct 116

Silencing the wild-type and mutant K-ras increases the resistance to 5-flurouracil in HCT-116 as a colorectal cancer cell line

2015 ◽  
Vol 26 (2) ◽  
pp. 187-196 ◽  
Author(s):  
Ladan Teimoori-Toolabi ◽  
Saba Hashemi ◽  
Kayhan Azadmanesh ◽  
Farnaz Eghbalpour ◽  
Farnaz Safavifar ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Line ◽  
Wild Type ◽  
Hct 116

Proliferative Effect of FadA Recombinant Protein from Fusobacterium nucleatum on SW480 Colorectal Cancer Cell Line

2020 ◽  
Vol 20 ◽  
Author(s):  
Masoud Dadashi ◽  
Bahareh Hajikhani ◽  
Ebrahim faghihloo ◽  
Parviz Owlia ◽  
Somayeh Yaslianifard ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Fusobacterium Nucleatum ◽  
Cell Counting ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Line ◽  
Proliferative Effect ◽  
Counting Methods

Background and Aim: Colorectal Cancer (CRC) is one of the most frequent cancers diagnosed in both men and women worldwide. Fusobacterium nucleatum adhesin A (FadA) has an important potential factor in the development or progression of CRC. The aim of the present study was to evaluate the proliferative effect of recombinant FadA on SW480 colorectal cancer cell line. Materials and Methods: The recombinant pET21(b)-fadA plasmid was synthesized and transformed into competent E.coli DH5α. In the next step, induction and expression of recombinant FadA were carried out in E. coli BL21 (DE3) competent cells. Expression and purification of protein were successfully done and it was analyzed and confirmed by SDS-PAGE and western blotting. The proliferative effect of purified FadA on SW480 CRC cell line was evaluated using MTT assay and cell counting methods. Results: Visualization of the specific band isolated from the linear plasmid on the agarose gel confirmed the presence of the desired gene. After electrophoresis and Coomassie blue staining, the protein of interest with an approximate molecular weight of 13KDa was detected. The MTT assay, similar to cell counting methods, revealed that FadA dose and timedependently promoted SW480 cell growth and proliferation in 24, 48 and 72 hours. Conclusion: The results showed that FadA stimulates proliferation of SW480 colorectal cancer cell line with a dose and time-dependent manner.

scholarly journals Anti-migration Effect of Aaptos suberitoides Fraction in HCT-116 Colorectal Cancer Cell Line

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Dany Muhammad Daffa ◽  
Muhammad Hasan Bashari ◽  
Eko Fuji Ariyanto ◽  
Tenny Putri ◽  
Nurul Qomarilla
Keyword(s):  
Colorectal Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Cellular Migration ◽  
Colorectal Cancer Cell Line ◽  
Migration Inhibition ◽  
Migration Activity ◽  
Migration Effect ◽  
Hct 116

Background: Colorectal cancer is the second leading cause of mortality and the most prevalent cancer worldwide. Most patients, who come with late-stage, have ineffective treatments and some side effects in chemotherapy. Aaptos suberitoides has potential anti-cancer effects due to its bioactive compounds such as aptamine. This study aimed to evaluate the migration inhibition effect of Aaptos suberitoides fraction in HCT-116 cell line.Methods: This study was an experimental study. Aaptos suberitoides specimen was taken in Tinjil Island and fractionated with ethyl acetate. HCT-116 cell line was added with Aaptos suberitoides fraction and cellular migration activity was observed in 48 hours of which the scratch assay was performed. The gap closure area was determined with ImageJ software.Results: The data showed that a low concentration of Aaptos suberitoides fraction inhibited migration activity in HCT-116 cell line as follow; 1 and 5 mg/L Aaptos suberitoides fraction inhibit 3-4 % cancer cell migration in 24 hours, and 10-11% inhibition in 48 hours, respectively. However, 10 mg/L fraction concentration only inhibited 7-14% of the migration effect.Conclusion: Aaptos suberitoides fraction suggests insignificant migration inhibition in colorectal cancer cells and only inhibits less than 15 % HCT-116 cell line.

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