Monochloramine suppresses the proliferation of colorectal cancer cell line Caco-2 by both apoptosis and G2/M cell cycle arrest

2013 ◽  
Vol 32 (2) ◽  
pp. 188-193 ◽  
Author(s):  
Tetsuya Kohda ◽  
Satoru Sakuma ◽  
Muneyuki Abe ◽  
Yohko Fujimoto
Keyword(s):  
Colorectal Cancer ◽  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Line ◽  
M Cell ◽  
Cycle Arrest

Ganoderma tsugae extracts inhibit colorectal cancer cell growth via G2/M cell cycle arrest

2008 ◽  
Vol 120 (3) ◽  
pp. 394-401 ◽  
Author(s):  
Shih-Chung Hsu ◽  
Chien-Chih Ou ◽  
Jhy-Wei Li ◽  
Tzu-Chao Chuang ◽  
Han-Pon Kuo ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Cycle ◽  
Cell Growth ◽  
Cell Cycle Arrest ◽  
Cancer Cell ◽  
Colorectal Cancer Cell ◽  
M Cell ◽  
Cancer Cell Growth ◽  
Cycle Arrest

A Comparison of the Anti-Cancer Effects of Free and PLGA-PAA Encapsulated Hydroxytyrosol on the HT-29 Colorectal Cancer Cell Line

2021 ◽  
Vol 21 ◽  
Author(s):  
Nasrin S. Sani ◽  
Habib Onsori ◽  
Somayeh Akrami ◽  
Mohammad Rahmati
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Line ◽  
Cycle Arrest ◽  
Anti Cancer ◽  
Colorectal Cancer Cells

Background: Hydroxytyrosol is one of the phenolic compounds of olive oil and can induce anti-cancer effects on the colorectal cancer cells. Objective: The aim of the present study was to evaluate the free hydroxytyrosol and nano-capsulated hydroxytyrosol effects on the cell cycle arrest in HT-29 colorectal cancer cell line. Methods: The nano-capsulated hydroxytyrosol was synthesized in poly lactide-co-glycolide-co-polyacrylic acid (PLGA-PAA) copolymer. MTT assay was performed to evaluate the anti- proliferative and anti-tumor effects of the free hydroxytyrosol and nano-capsulated hydroxytyrosol. Finally, the relative expression of CDKN1A, CDKN1B and CCND1 genes was evaluated in the control and treated colorectal cancer cells by using Real-Time PCR. Results: The obtained results from the MTT assay showed that the cytotoxic effects of the nano-capsulated hydroxytyrosol on the colorectal cancer cell line (IC50= 6PPM) was significantly more than free hydroxytyrosol (IC50= 12PPM) after 72h. Also, nano-capsulated hydroxytyrosol showed more significant effects on the up-regulation of CDKN1A and CDKN1B genes, and down-regulation of the CCND1 gene in the colorectal cancer cells. Conclusion: In conclusion, the present study showed that the hydroxytyrosol led to die the colorectal cancer cell through the cell cycle arrest. Also, the PLGA-PAA copolymer dramatically caused to increase the cytotoxic effects of the hydroxytyrosol on the colorectal cancer cells.

scholarly journals Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin

2009 ◽  
Vol 33 (2) ◽  
pp. 155-160 ◽  
Author(s):  
KetLi Ho ◽  
Latifah Saiful Yazan ◽  
Norsharina Ismail ◽  
Maznah Ismail
Keyword(s):  
Colorectal Cancer ◽  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cell Line ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell Line ◽  
Human Colorectal Cancer ◽  
Cycle Arrest ◽  
Human Colorectal Cancer Cell ◽  
Ht 29

New synthetic sulfonamide chalcone induced cell cycle arrest and cell death in colorectal adenocarcinoma metastatic cells (SW-620)

2021 ◽  
Vol 21 ◽  
Author(s):  
Andréa Felinto Moura ◽  
Mirian Rita Carrilho de Castro ◽  
Raquel Ferreira Naves ◽  
Ana Jérsia Araújo ◽  
Maria Claudia Luciano dos Santos ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Cycle ◽  
Cell Death ◽  
Cell Cycle Arrest ◽  
Cancer Cell ◽  
Cytotoxic Effect ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Line ◽  
Dependent Manner ◽  
Cycle Arrest

Background: New chalcones have been developed from the insertion of organic groups, among them sulfonamides, presenting varied biological activity. Objective: The aim of this work was to determine the antitumor potential of a new synthetic sulfonamide chalcone (SSC185) against a colorectal metastatic lymph node-derived colorectal cancer cell line (SW-620). Method: Synthesis and characterization, including crystallography, of SSC185 were performed. SSC185 showed a selective cytotoxic effect against colorectal cancer cell lines. Therefore, the cytotoxic effect of SSC185 against SW-620 was further investigated. We used optical and fluorescence microscopy, flow cytometry and Western blot to determine the antitumor effects of SSC185. Results: SSC185 induced cytotoxicity in SW-620 cells in a time and concentration-dependent manner. Cell cycle progression was disrupted, with increased G2/M cell number and consequent cell death, with morphological alterations associated with apoptosis and necrosis. Cell death was associated with the activation and cleavage of PARP, and with reduced expression of the pro-apoptotic Bax protein and caspase 8, depending on the SSC185 concentration tested. Expression of the necroptosis pathway proteins RIP and MLKL was also reduced. These proteins are phosphorylated during the process of necroptosis. Conclusion: We suggest that the mechanism involved in the cytotoxic effect of SSC185 against SW-620 in vitro may be related to the induction of cell cycle arrest in the G2/M phase and cell death by apoptosis or necroptosis, depending on the concentration used.

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