Calcium hypochlorite on mouse embryonic fibroblast cells (NIH3T3) in vitro cytotoxicity and genotoxicity: MTT and comet assay

2020 ◽  
Vol 47 (7) ◽  
pp. 5377-5383
Author(s):  
Şehnaz Yilmaz ◽  
Oguz Yoldas ◽  
Aysin Dumani ◽  
Gizem Guler ◽  
Seda Ilgaz ◽  
...  
Keyword(s):  
Comet Assay ◽  
Mouse Embryonic Fibroblast ◽  
In Vitro Cytotoxicity ◽  
Fibroblast Cells ◽  
Calcium Hypochlorite ◽  
Embryonic Fibroblast

scholarly journals In Vitro Evaluation of the Biocompatibility and Stability of Fluorescent Dil Dye on Mouse Embryonic Fibroblast Cells

2019 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Marzyeh Haghshenas ◽  
Elham Hoveizi ◽  
Tayebeh Mohammadi ◽  
Seyed Reza Kazemi Nezhad
Keyword(s):  
Mouse Embryonic Fibroblast ◽  
Fibroblast Cells ◽  
In Vitro Evaluation ◽  
Embryonic Fibroblast

Ectopic overexpression of Nanog induces tumorigenesis in non-tumorous fibroblasts

2016 ◽  
Vol 397 (3) ◽  
pp. 249-255 ◽  
Author(s):  
Yo Seph Park ◽  
Judee Grace E. Nemeño ◽  
Na Young Choi ◽  
Jeong Ik Lee ◽  
Kisung Ko ◽  
...  
Keyword(s):  
Stem Cells ◽  
Morphological Changes ◽  
Embryonic Stem ◽  
Mouse Embryonic Fibroblast ◽  
Tumor Formation ◽  
Proliferation Rate ◽  
Fibroblast Cells ◽  
Reprogramming Factors ◽  
Embryonic Fibroblast

Abstract Key regulatory genes in pluripotent stem cells are of interest not only as reprogramming factors but also as regulators driving tumorigenesis. Nanog is a transcription factor involved in the maintenance of embryonic stem cells and is one of the reprogramming factors along with Oct4, Sox2, and Lin28. Nanog expression has been detected in different types of tumors, and its expression is a poor prognosis for cancer patients. However, there is no clear evidence that Nanog is functionally involved in tumorigenesis. In this study, we induced overexpression of Nanog in mouse embryonic fibroblast cells and subsequently assessed their morphological changes, proliferation rate, and tumor formation ability. We found that Nanog overexpression induced immortalization of mouse embryonic fibroblast cells (MEFs) and increased their proliferation rate in vitro. We also found that formation of tumors after subcutaneous injection of retroviral-Nanog infected MEFs (N-MEFs) into athymic mouse. Cancer-related genes such as Bmi1 were expressed at high levels in N-MEFs. Hence, our results demonstrate that Nanog is able to transform normal somatic cells into tumor cells.

scholarly journals Slow growth and unstable ribosomal RNA lacking pseudouridine in mouse embryonic fibroblast cells expressing catalytically inactive dyskerin

FEBS Letters ◽  
2013 ◽  
Vol 587 (14) ◽  
pp. 2112-2117 ◽  
Author(s):  
Bai-Wei Gu ◽  
Jingping Ge ◽  
Jian-Meng Fan ◽  
Monica Bessler ◽  
Philip J. Mason
Keyword(s):  
Ribosomal Rna ◽  
Slow Growth ◽  
Mouse Embryonic Fibroblast ◽  
Fibroblast Cells ◽  
Embryonic Fibroblast

scholarly journals Effect of BIX-01294 on H3K9me2 levels and the imprinted gene Snrpn in mouse embryonic fibroblast cells

2015 ◽  
Vol 35 (5) ◽  
Author(s):  
Peng Chen ◽  
Jian-Feng Yao ◽  
Rong-Fu Huang ◽  
Fang-Fang Zheng ◽  
Xiao-Hong Jiang ◽  
...  
Keyword(s):  
Epigenetic Regulation ◽  
Biological Effects ◽  
Mouse Embryonic Fibroblast ◽  
Epigenetic Modifications ◽  
Fibroblast Cells ◽  
Imprinted Gene ◽  
Amine Derivative ◽  
Small Nuclear Ribonucleoprotein ◽  
Nuclear Ribonucleoprotein ◽  
Embryonic Fibroblast

BIX-01294 (a diazepin-quinazolin-amine derivative) has important biological effects and its epigenetic regulation at imprinting control regions is highly complex. BIX-01294 may reduce global H3K9me2 levels and affect epigenetic modifications of small nuclear ribonucleoprotein N (Snrpn) in MEFs.

scholarly journals Preparation and evaluation of chitosan based thermoreversible gels for intraperitoneal delivery of 5-fluorouracil (5-FU)

2013 ◽  
Vol 63 (4) ◽  
pp. 479-491 ◽  
Author(s):  
Bhavesh P. Depani ◽  
Anuja A. Naik ◽  
Hema A. Nair
Keyword(s):  
Antineoplastic Agent ◽  
In Vitro Cytotoxicity ◽  
In Vivo Studies ◽  
Comparable Efficacy ◽  
Prolonged Release ◽  
Fibroblast Cells ◽  
Glycerol Phosphate ◽  
Drug Free

Abstract Sterile thermoreversibly gelling systems based on chitosan- glycerol phosphate were developed for intraperitoneal delivery of the antineoplastic agent 5-FU. The formulation was evaluated for gelling characteristics and in vitro drug release. Drug free gels were evaluated for in vitro cytotoxicity in L-929 mouse fibroblast cells. Drug loaded gels were subjected to acute toxicity studies in Swiss albino mice via intraperitoneal route and efficacy studies via intratumoral injections in subcutaneous colon carcinoma bearing BALB/c mice. The formulations gelled reversibly in 8 min at 37 °C and provided prolonged release of the drug. Drug free systems showed dose dependent cytotoxicity in fibroblast cells, while in vivo studies revealed a 2.8-fold increase in LD50 of 5-FU administered intraperitoneally as the developed system. Tumor volume measurements showed comparable efficacy of 5-FU administered as gel and commercial injection with a greatly improved safety profile of the former as adjudged from mortality and body weight measurements.

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