Arthrospira platensis transglutaminase derived antioxidant peptide-packed electrospun chitosan/ poly (vinyl alcohol) nanofibrous mat accelerates wound healing, in vitro, via inducing mouse embryonic fibroblast proliferation

2020 ◽  
Vol 193 ◽  
pp. 111124
Author(s):  
Anbazahan Sannasimuthu ◽  
Madhura Ramani ◽  
Bilal Ahamad Paray ◽  
Mukesh Pasupuleti ◽  
Mohammad K. Al-Sadoon ◽  
...  
Keyword(s):  
Wound Healing ◽  
Vinyl Alcohol ◽  
Arthrospira Platensis ◽  
Mouse Embryonic Fibroblast ◽  
Poly Vinyl Alcohol ◽  
Fibroblast Proliferation ◽  
Antioxidant Peptide ◽  
Embryonic Fibroblast

Electrospun poly(vinyl) alcohol/collagen nanofibrous scaffold hybridized by graphene oxide for accelerated wound healing

2018 ◽  
Vol 41 (8) ◽  
pp. 467-473 ◽  
Author(s):  
Rethinam Senthil ◽  
Robert Berly ◽  
Thimmiah Bhargavi Ram ◽  
Nallathambi Gobi
Keyword(s):  
Wound Healing ◽  
Graphene Oxide ◽  
Vinyl Alcohol ◽  
Collagen Type ◽  
Collagen Type I ◽  
Fish Bone ◽  
Poly Vinyl Alcohol ◽  
Type I

Purpose: In this study, a blend of synthetic polymer (poly(vinyl) alcohol), natural polymer (collagen type I from fish bone), and graphene oxide nanoparticles is used to fabricate a composite nanofibrous scaffold, by electrospinning, for their potential application in accelerated wound healing. Methods: The scaffold was characterized for its physicochemical and mechanical properties. In vitro studies were carried out using human keratinocyte cell line (HaCaT) which proved the biocompatibility of the scaffold. In vivo study using mice model was carried out and the healing pattern was evaluated using histopathological studies. Results: Scaffold prepared from poly(vinyl) alcohol, collagen type I from fish bone, and graphene oxide possessed better physicochemical and mechanical properties. In addition, in vivo and in vitro studies showed its accelerated wound healing properties. Conclusion: The scaffold with required strength and biocompatibility may be tried as a wound dressing material in large animals after getting necessary approval.

Bi-Layer Composite Dressing of Gelatin Nanofibrous Mat and Poly Vinyl Alcohol Hydrogel for Drug Delivery and Wound Healing Application:In-Vitro and In-Vivo Studies

2013 ◽  
Vol 9 (9) ◽  
pp. 1495-1508 ◽  
Author(s):  
Maneesh Jaiswal ◽  
Asheesh Gupta ◽  
Ashwini K. Agrawal ◽  
Manjeet Jassal ◽  
Amit Kr. Dinda ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Wound Healing ◽  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
Layer Composite

scholarly journals Fabrication of Hybrid Nanofibers from Biopolymers and Poly (Vinyl Alcohol)/Poly (ε-Caprolactone) for Wound Dressing Applications

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2104
Author(s):  
Sibusiso Alven ◽  
Blessing Atim Aderibigbe
Keyword(s):  
Mechanical Properties ◽  
Wound Healing ◽  
Vinyl Alcohol ◽  
Wound Dressing ◽  
Chronic Wounds ◽  
Cellular Adhesion ◽  
Wound Dressings ◽  
Poly Vinyl Alcohol ◽  
High Porosity ◽  
Hybrid Nanofibers

The management of chronic wounds is challenging. The factors that impede wound healing include malnutrition, diseases (such as diabetes, cancer), and bacterial infection. Most of the presently utilized wound dressing materials suffer from severe limitations, including poor antibacterial and mechanical properties. Wound dressings formulated from the combination of biopolymers and synthetic polymers (i.e., poly (vinyl alcohol) or poly (ε-caprolactone) display interesting properties, including good biocompatibility, improved biodegradation, good mechanical properties and antimicrobial effects, promote tissue regeneration, etc. Formulation of these wound dressings via electrospinning technique is cost-effective, useful for uniform and continuous nanofibers with controllable pore structure, high porosity, excellent swelling capacity, good gaseous exchange, excellent cellular adhesion, and show a good capability to provide moisture and warmth environment for the accelerated wound healing process. Based on the above-mentioned outstanding properties of nanofibers and the unique properties of hybrid wound dressings prepared from poly (vinyl alcohol) and poly (ε-caprolactone), this review reports the in vitro and in vivo outcomes of the reported hybrid nanofibers.

scholarly journals Dual Network Composites of Poly(vinyl alcohol)-Calcium Metaphosphate/Alginate with Osteogenic Ions for Bone Tissue Engineering in Oral and Maxillofacial Surgery

2021 ◽  
Vol 8 (8) ◽  
pp. 107
Author(s):  
Lilis Iskandar ◽  
Lucy DiSilvio ◽  
Jonathan Acheson ◽  
Sanjukta Deb
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Vinyl Alcohol ◽  
Maxillofacial Surgery ◽  
Bone Defects ◽  
Bone Tissue Regeneration ◽  
Poly Vinyl Alcohol ◽  
Oral And Maxillofacial Surgery

Despite considerable advances in biomaterials-based bone tissue engineering technologies, autografts remain the gold standard for rehabilitating critical-sized bone defects in the oral and maxillofacial (OMF) region. A majority of advanced synthetic bone substitutes (SBS’s) have not transcended the pre-clinical stage due to inferior clinical performance and translational barriers, which include low scalability, high cost, regulatory restrictions, limited advanced facilities and human resources. The aim of this study is to develop clinically viable alternatives to address the challenges of bone tissue regeneration in the OMF region by developing ‘dual network composites’ (DNC’s) of calcium metaphosphate (CMP)—poly(vinyl alcohol) (PVA)/alginate with osteogenic ions: calcium, zinc and strontium. To fabricate DNC’s, single network composites of PVA/CMP with 10% (w/v) gelatine particles as porogen were developed using two freeze–thawing cycles and subsequently interpenetrated by guluronate-dominant sodium alginate and chelated with calcium, zinc or strontium ions. Physicochemical, compressive, water uptake, thermal, morphological and in vitro biological properties of DNC’s were characterised. The results demonstrated elastic 3D porous scaffolds resembling a ‘spongy bone’ with fluid absorbing capacity, easily sculptable to fit anatomically complex bone defects, biocompatible and osteoconductive in vitro, thus yielding potentially clinically viable for SBS alternatives in OMF surgery.

scholarly journals Multifunctional Coatings for Robotic Implanted Device

2019 ◽  
Vol 20 (20) ◽  
pp. 5126 ◽  
Author(s):  
Caterina Cristallini ◽  
Serena Danti ◽  
Bahareh Azimi ◽  
Veronika Tempesti ◽  
Claudio Ricci ◽  
...  
Keyword(s):  
Acrylic Acid ◽  
Vinyl Alcohol ◽  
Soft Tissues ◽  
Functional Characterization ◽  
Titanium Substrate ◽  
Repair Process ◽  
Chemical Imaging ◽  
In Vitro Cytotoxicity ◽  
Poly Vinyl Alcohol

The objective of this study was the preparation and physico-chemical, mechanical, biological, and functional characterization of a multifunctional coating for an innovative, fully implantable device. The multifunctional coating was designed to have three fundamental properties: adhesion to device, close mechanical resemblance to human soft tissues, and control of the inflammatory response and tissue repair process. This aim was fulfilled by preparing a multilayered coating based on three components: a hydrophilic primer to allow device adhesion, a poly(vinyl alcohol) hydrogel layer to provide good mechanical compliance with the human tissue, and a layer of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) fibers. The use of biopolymer fibers offered the potential for a long-term interface able to modulate the release of an anti-inflammatory drug (dexamethasone), thus contrasting acute and chronic inflammation response following device implantation. Two copolymers, poly(vinyl acetate-acrylic acid) and poly(vinyl alcohol-acrylic acid), were synthetized and characterized using thermal analysis (DSC, TGA), Fourier transform infrared spectroscopy (FT-IR chemical imaging), in vitro cell viability, and an adhesion test. The resulting hydrogels were biocompatible, biostable, mechanically compatible with soft tissues, and able to incorporate and release the drug. Finally, the multifunctional coating showed a good adhesion to titanium substrate, no in vitro cytotoxicity, and a prolonged and controlled drug release.

Tailoring the in vitro characteristics of poly(vinyl alcohol)-nanohydroxyapatite composite scaffolds for bone tissue engineering

2016 ◽  
Vol 36 (8) ◽  
pp. 771-784 ◽  
Author(s):  
Tejinder Kaur ◽  
Arunachalam Thirugnanam ◽  
Krishna Pramanik
Keyword(s):  
Mechanical Strength ◽  
Vinyl Alcohol ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Poly Vinyl Alcohol ◽  
Apatite Formation ◽  
Composite Scaffolds ◽  
Alizarin Red ◽  
Casting Technique

Abstract Poly(vinyl alcohol) reinforced with nanohydroxyapatite (PVA-nHA) composite scaffolds were developed by varying the nHA (1%, 2%, 3%, 4%, and 5%, w/v) composition in the PVA matrix by solvent casting technique. The developed composite scaffolds were characterized using scanning electron microscopy (SEM), X-ray powder diffraction (XRD), attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, and contact angle measurement. The stability of the composite scaffolds in physiological environment was evaluated by swelling and degradation studies. Further, these composite scaffolds were tested for in vitro bioactivity, hemolysis, biocompatibility, and mechanical strength. SEM micrographs showed a homogenous distribution of nHA (3%, w/v) in the PVA matrix. XRD and ATR-FTIR analysis confirmed no phase contamination and the existence of the chemical bond between PVA-nHA at approximately 2474 cm-1. PVA-nHA composite scaffolds with 3% (w/v) concentration of nHA showed nominal swelling and degradation behavior with good mechanical strength. The mechanical strength and degradation properties of the scaffold above 3% (w/v) of nHA was found to deteriorate, which is due to the agglomeration of nHA. The in vitro bioactivity and hemolysis studies showed improved apatite formation and hemocompatibility of the developed scaffolds. In vitro cell adhesion, proliferation, alkaline phosphatase activity, and Alizarin red S staining confirmed the biocompatibility of the composite scaffolds.

scholarly journals The Design, Characterization and Antibacterial Activity of Heat and Silver Crosslinked Poly(Vinyl Alcohol) Hydrogel Forming Dressings Containing Silver Nanoparticles

Nanomaterials ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 96
Author(s):  
John Jackson ◽  
Helen Burt ◽  
Dirk Lange ◽  
In Whang ◽  
Robin Evans ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Silver Nitrate ◽  
Vinyl Alcohol ◽  
Unmet Need ◽  
Chemical Characterization ◽  
Wound Dressings ◽  
Poly Vinyl Alcohol ◽  
Burn Wound

The prompt treatment of burn wounds is essential but can be challenging in remote parts of Africa, where burns from open fires are a constant hazard for children and suitable medical care may be far away. Consequently, there is an unmet need for an economical burn wound dressing with a sustained antimicrobial activity that might be manufactured locally at low cost. This study describes and characterizes the novel preparation of a silver nitrate-loaded/poly(vinyl alcohol) (PVA) film. Using controlled heating cycles, films may be crosslinked with in situ silver nanoparticle production using only a low heat oven and little technical expertise. Our research demonstrated that heat-curing of PVA/silver nitrate films converted the silver to nanoparticles. These films swelled in water to form a robust, wound-compatible hydrogel which exhibited controlled release of the antibacterial silver nanoparticles. An optimal formulation was obtained using 5% (w/w) silver nitrate in PVA membrane films that had been heated at 140 °C for 90 min. Physical and chemical characterization of such films was complemented by in vitro studies that confirmed the effective antibacterial activity of the released silver nanoparticles against both gram positive and negative bacteria. Overall, these findings provide economical and simple methods to manufacture stable, hydrogel forming wound dressings that release antibiotic silver over prolonged periods suitable for emergency use in remote locations.

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