scholarly journals Mutational landscape of paired primary and synchronous metastatic lymph node in chemotherapy naive gallbladder cancer

2022 ◽  
Author(s):  
Boqiang Fan ◽  
Xianfeng Xu ◽  
Xuehao Wang
Keyword(s):  
Lymph Node ◽  
Gallbladder Cancer ◽  
Metastatic Lymph Node ◽  
Mutational Landscape ◽  
Metastatic Lymph

scholarly journals Application of nomogram containing log odds of metastatic lymph node in gallbladder cancer patients

2020 ◽  
Vol 8 (10) ◽  
pp. 655-655
Author(s):  
Zhan Shi ◽  
Zunqiang Xiao ◽  
Lijie Li ◽  
Linjun Hu ◽  
Yuling Gao ◽  
...  
Keyword(s):  
Lymph Node ◽  
Gallbladder Cancer ◽  
Cancer Patients ◽  
Metastatic Lymph Node ◽  
Metastatic Lymph ◽  
Log Odds

scholarly journals Mutational Landscape of Paired Primary and Synchronous Metastatic Lymph Node in Chemotherapy Naive Gallbladder Cancer

2021 ◽  
Author(s):  
Boqiang Fan ◽  
Xianfeng Xu ◽  
Xuehao Wang
Keyword(s):  
Phylogenetic Analysis ◽  
Lymph Node ◽  
Gallbladder Cancer ◽  
Primary Tumor ◽  
Copy Number ◽  
Metastatic Lymph Node ◽  
Copy Number Variations ◽  
Driver Mutations ◽  
Primary Tumors ◽  
Metastatic Lymph

Abstract Background: Comprehensive genomic analysis of paired primary tumors and their metastatic lesions may provide new insights into the biology of metastatic processes and therefore guide the development of novel strategies for intervention. To date, our knowledge of the genetic divergence and phylogenetic relationships in gallbladder cancer (GBC) is limited.Methods:We performed whole exome sequencing (WES) for 5 patients with primary tumor, metastatic lymph node (LNM) and corresponding normal tissue. Mutations, mutation signatures and copy number variations were analyzed with state-of-art bioinformatics methods. Phylogenetic tree was also generated to infer metastatic pattern. Results:Five driver mutations were detected in these patients. Among which, TP53 was the only shared mutation between primary tumor and LNM. Although tumor mutational burden was comparable between primary tumor and LNM, higher mutation burden was observed in LNM of one patient. Copy number variations (CNVs) burden was higher in LNM than their primary tumor. Phylogenetic analysis indicated both linear and parallel progression of metastasis exist in these patients. TP53 mutation and CNVs were homogenously between primary tumor and LNM.Conclusions:High consistence of genetic landscape were shown between primary tumor and LNM in GBC. However, heterogenicity still exist between primary tumor and LNM in particular patients in term of driver mutation, TMB and CNV burden. Phylogenetic analysis indicated both Linear and parallel progression of metastasis were exist among these patients.

236 Evaluation of PD-L1 expression in primary lung tumor and metastatic lymph nodes in the presence of immune cells

2020 ◽  
Vol 8 (Suppl 3) ◽  
pp. A253-A253
Author(s):  
Chris Hansis ◽  
Xiaomei Wang ◽  
Tao Wang ◽  
Gerald Feldman
Keyword(s):  
Lung Cancer ◽  
Squamous Cell Carcinoma ◽  
Lymph Node ◽  
Tumor Cells ◽  
Squamous Cell ◽  
Immune Cells ◽  
Lymph Node Metastases ◽  
Metastatic Lymph Node ◽  
Metastatic Lymph ◽  
Node Metastases

BackgroundImmunotherapies against programmed death ligand-1 (PD-L1) have been established as an effective treatment for a subset of lung cancer patients. Even though it is critical for a successful therapy to know prevalent PD-L1 expression patterns in all affected tissues, information on matching lymph node metastases and immune cells is particularly limited. The purpose of this study was thus to evaluate comparative PD-L1 expression profiles in those tissues.MethodsFDA-approved IHC assays for PD-L1 (Dako 22C3) were performed on a lung tissue array (LC814A, US Biomax) according to manufacturer’s instructions. Histopathological analysis by H-scoring was performed to determine the rate and intensity of positive tumor and immune cell staining for each of the 80 cores. The H score was calculated as follows: A total of up to 300 cells were assessed, per specimen, at 40x high-power magnification (typically over 7–10 fields). A staining level of 0–3 was then assigned to each cell, to designate the intensity of specific positive membranous-to-cytoplasmic staining. The H score was subsequently calculated as% cells staining at level 1 (x1) +% cells staining at level 2 (x2) +% cells staining at level 3 (x3) = total H score per sample. This resulted in a maximum possible H score of 300.ResultsOf the 16 adenocarcinoma tumor samples with a valid staining, 7 (44%) showed positive PD-L1 staining for tumor cells and 10 (63%) for primary immune cells. Importantly, 9 matching metastatic lymph node samples out of the 16 samples (56%) showed an increased PD-L1 H score compared to primary tumors for both tumor cells and immune cells (figure 1). Of the 15 squamous cell carcinoma samples with a valid staining, 11 (73%) showed detectable PD-L1 expression levels in the primary tumor and 12 (80%) in the primary immune cells, while 7 (47%) and 9 (60%) showed lower scores in matching metastatic lymph node tumor cells and their immune cells, respectively (figure 2). Very low or no expression of PD-L1 was detected in small cell lung cancer, as to be expected from previous studies.Abstract 236 Figure 1PD-L1 Staining in adenocarcinomaAbstract 236 Figure 2PD-L1 Staining in squamous cell carcinomaConclusionsSquamous cell carcinomas and adenocarcinomas display significant heterogeneity with regard to PD-L1 expression in associated lymph node metastases. While the reasons for this frequent discordant PD-L1 expression pattern involving both tumor and immune cells need to be investigated further, our findings may help guide the proper interpretation of PD-L1 companion diagnostic test results and subsequent therapeutic decisions.AcknowledgementsThe views in this Abstract have not been formally disseminated by the U.S. Food and Drug Administration and should not be construed to represent any agency determination or policy.

Automatic FDG-PET-based tumor and metastatic lymph node segmentation in cervical cancer

2014 ◽  
Author(s):  
Dídac R. Arbonès ◽  
Henrik G. Jensen ◽  
Annika Loft ◽  
Per Munck af Rosenschöld ◽  
Anders Elias Hansen ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Lymph Node ◽  
Fdg Pet ◽  
Metastatic Lymph Node ◽  
Metastatic Lymph
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