Effects of valerate on intestinal barrier function in cultured Caco-2 epithelial cell monolayers

2021 ◽  
Author(s):  
Guanzhen Gao ◽  
Jingru Zhou ◽  
Huiqin Wang ◽  
Yanan Ding ◽  
Jianwu Zhou ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Barrier Function ◽  
Intestinal Barrier ◽  
Intestinal Barrier Function ◽  
Cell Monolayers ◽  
Epithelial Cell Monolayers

scholarly journals Arctigenin fromFructus Arctii(Seed of Burdock) Reinforces Intestinal Barrier Function in Caco-2 Cell Monolayers

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Hee Soon Shin ◽  
Sun Young Jung ◽  
Su Yeon Back ◽  
Jeong-Ryong Do ◽  
Dong-Hwa Shon
Keyword(s):  
Barrier Function ◽  
Active Component ◽  
Inflammatory Diseases ◽  
Intestinal Barrier ◽  
Transepithelial Electrical Resistance ◽  
Intestinal Barrier Function ◽  
Intestinal Epithelial ◽  
Traditional Herbal Medicine ◽  
Cell Monolayers ◽  
Basolateral Side

Fructus Arctiiis used as a traditional herbal medicine to treat inflammatory diseases in oriental countries. This study aimed to investigate effect ofF. Arctiiextract on intestinal barrier function in human intestinal epithelial Caco-2 cells and to reveal the active component ofF. Arctii. We measured transepithelial electrical resistance (TEER) value (as an index of barrier function) and ovalbumin (OVA) permeation (as an index of permeability) to observe the changes of intestinal barrier function. The treatment ofF. Arctiiincreased TEER value and decreased OVA influx on Caco-2 cell monolayers. Furthermore, we found that arctigenin as an active component ofF. Arctiiincreased TEER value and reduced permeability of OVA from apical to the basolateral side but not arctiin. In the present study, we revealed thatF. Arctiicould enhance intestinal barrier function, and its active component was an arctigenin on the functionality. We expect that the arctigenin fromF. Arctiicould contribute to prevention of inflammatory, allergic, and infectious diseases by reinforcing intestinal barrier function.

scholarly journals TNFAIP3 Maintains Intestinal Barrier Function and Supports Epithelial Cell Tight Junctions

PLoS ONE ◽  
2011 ◽  
Vol 6 (10) ◽  
pp. e26352 ◽  
Author(s):  
Lauren E. Kolodziej ◽  
James P. Lodolce ◽  
Jonathan E. Chang ◽  
Jeffrey R. Schneider ◽  
Wesley A. Grimm ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Tight Junctions ◽  
Barrier Function ◽  
Intestinal Barrier ◽  
Intestinal Barrier Function

scholarly journals Research Communication: Glutamine and Barrier Function in Cultured Caco-2 Epithelial Cell Monolayers

2003 ◽  
Vol 133 (7) ◽  
pp. 2176-2179 ◽  
Author(s):  
Vincent G. DeMarco ◽  
Nan Li ◽  
Justin Thomas ◽  
Christopher M. West ◽  
Josef Neu
Keyword(s):  
Epithelial Cell ◽  
Barrier Function ◽  
Cell Monolayers ◽  
Research Communication ◽  
Epithelial Cell Monolayers

Collagen peptides ameliorate intestinal epithelial barrier dysfunction in immunostimulatory Caco-2 cell monolayers via enhancing tight junctions

2017 ◽  
Vol 8 (3) ◽  
pp. 1144-1151 ◽  
Author(s):  
Qianru Chen ◽  
Oliver Chen ◽  
Isabela M. Martins ◽  
Hu Hou ◽  
Xue Zhao ◽  
...  
Keyword(s):  
Barrier Function ◽  
Intestinal Barrier ◽  
Intestinal Barrier Function ◽  
Epithelial Barrier ◽  
Intestinal Epithelial ◽  
Barrier Dysfunction ◽  
Intestinal Epithelial Barrier ◽  
Cell Monolayers ◽  
Collagen Peptides ◽  
Epithelial Barrier Dysfunction

Alaska pollock skin derived collagen peptides could be considered as dietary supplements for intestinal barrier function promotion and associated diseases.

Glutamine regulates Caco-2 cell tight junction proteins

2004 ◽  
Vol 287 (3) ◽  
pp. G726-G733 ◽  
Author(s):  
Nan Li ◽  
Patricia Lewis ◽  
Don Samuelson ◽  
Kellym Liboni ◽  
Josef Neu
Keyword(s):  
Protein Expression ◽  
Tight Junction ◽  
Barrier Function ◽  
Cellular Localization ◽  
Intestinal Barrier ◽  
Intestinal Barrier Function ◽  
Intestinal Cell ◽  
Methionine Sulfoximine ◽  
Junctional Complex ◽  
Cell Monolayers

Intestinal epithelial tight junction (TJ) barrier dysfunction may lead to inflammation and mucosal injury. Glutamine (GLN) plays a role in maintenance of intestinal barrier function in various animal models and critically ill humans. Recent evidence from intestinal cell monolayers indicates that GLN maintains transepithelial resistance and decreases permeability. The mechanisms of these effects remain undefined. We hypothesized that GLN affects proteins involved in the intercellular junctional complex. GLN availability was controlled in Caco-2 monolayers by addition to the medium and treatment with methionine sulfoximine (MSO) to inhibit glutamine synthetase (GS). Expression of TJ proteins, claudin-1, occludin, and zonula occluden (ZO)-1 was measured by immunoblotting. Localization of TJ proteins was evaluated by immunofluorescence light microscopy. Structure of TJ was determined by transmission electron microscopy (TEM). Deprivation of GLN decreased claudin-1, occludin, and ZO-1 protein expression and caused a disappearance of perijunctional claudin-1 and a reduction of occludin but had no effect on ZO-1. TEM revealed that MSO-treated cells in the absence of GLN formed irregular junctional complexes between the apical lateral margins of adjoining cells. These findings indicate that TJ protein expression and cellular localization in Caco-2 cell monolayers rely on GLN. This mechanism may similarly relate to GLN-mediated modulation of intestinal barrier function in stressed animals and humans.

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