scholarly journals Relationship of matrix stiffness and cell morphology in regulation of osteogenesis and adipogenesis of BMSCs

2022 ◽  
Author(s):  
Yutong Guo ◽  
Yini Qiao ◽  
Shuqi Quan ◽  
Cai Yang ◽  
Juan Li
Keyword(s):  
Cell Morphology ◽  
Matrix Stiffness ◽  
Relationship Of

scholarly journals Relationship of Matrix Stiffness and Cell Morphology in Regulation of Osteo Genesis and Adipogenesis of BMSCs

2021 ◽  
Author(s):  
Yutong Guo ◽  
Yini Qiao ◽  
Shuqi Quan ◽  
Cai Yang ◽  
Juan Li
Keyword(s):  
Tissue Culture ◽  
Cell Morphology ◽  
Actin Polymerization ◽  
Nuclear Translocation ◽  
Cell Spreading ◽  
The Other ◽  
Matrix Stiffness ◽  
Robust Regulation ◽  
Relationship Of ◽  
Rat Bone

Abstract Backgrounds: Both matrix stiffness and cell morphology have been found as important factors directing MSCs (mesenchymal stem cells) differentiation, but cells also spontaneously adapt their morphology under matrix stiffness stimulation. This study aimed to investigate the interplay of cell morphology and matrix stiffness on osteogenesis and adipogenesis of rBMSCs(rat bone BMSCs) on 2D substrates. Methods and Results: First, we modulated MSCs morphology through different fibronectin (FN) concentrations on tissue culture plates (TCPs). We found FN promoted and osteogenesis of BMSCs while suppressing adipogenesis, mediated by FN-induced F-actin polymerization and cell spreading. Based on these findings, we modulated BMSCs morphology on 0.5 kPa and 32 kPa CytoSoft® plates through FN concentrations. We found BMSCs on 0.5 kPa substrates coated with 300μg/ml of FN manifested similarly spreading morphology with cells on 32 kPa substrates coated with 100 μg/ml of FN, and cells in both groups dominantly commit osteogenesis. On the other hand, BMSCs on 32 kPa substrates coated with 30μg/ml of FN manifested similarly restricted morphology with cells in on 0.5 kPa substrates with 100μg/ml of FN, and in both groups cells mainly commit adipogenesis. Immunofluorescence staining indicated YAP/TAZ mainly located in cytoplasm when cells exhibited restricted morphology on stiff matrices, while exhibiting significant nuclear translocation when cells spread on soft matrices. Conclusions: Cell morphology overrode effects of matrix stiffness on BMSCs differentiation through more robust regulation of YAP/TAZ. Matrix stiffness depended on cell morphology to regulate osteogenesis and adipogenesis of BMSCs.

scholarly journals The relationship of fibroblast translocations to cell morphology and stress fibre density

1982 ◽  
Vol 53 (1) ◽  
pp. 21-36
Author(s):  
L. Lewis ◽  
J.M. Verna ◽  
D. Levinstone ◽  
S. Sher ◽  
L. Marek ◽  
...  
Keyword(s):  
Cell Morphology ◽  
Inverse Relationship ◽  
Human Fibroblasts ◽  
Time Lapse ◽  
Stress Fibers ◽  
Population Doubling ◽  
Fibre Density ◽  
Foregoing Observation ◽  
Relationship Of ◽  
The Relationship

Translocation of human fibroblasts in culture was studied using techniques of time-lapse cinemicrography, indirect immunofluorescence, and computer analysis. An inverse relationship between the velocity of cells during the last hour of life and the density of stress fibers seen by immune staining was demonstrated. Translocating cells generally assumed one of two interconvertible morphologies: a triangular tailed shape or tailed fibroblast (TF), and a tailless form that resembled a half-moon, which we call a half-moon fibroblast (HMF). The tail of TFs formed only on regions of substrate that had been previously traversed by cells. The half-moon morphology developed either on previously used or on virgin substrate. Cells adopted the HMF rather than the TF morphology with a four-fold greater frequency. HMFs translocated slightly faster than TFs. The foregoing observation suggest that the fibroblast tail is not an organelle essential for translocation. Since our technique allowed us to distinguish between cells which were cycling and those which had left cycle, we compared their velocities and found them to be similar. Also the average velocities of cells of different population-doubling levels (10th, 30th, 40th) were approximately equal.

scholarly journals Osteogenesis-Related Behavior of MC3T3-E1 Cells on Substrates with Tunable Stiffness

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Yingying Zhang ◽  
Yanghui Xing ◽  
Jian Li ◽  
Zhiqiang Zhang ◽  
Huiqin Luan ◽  
...  
Keyword(s):  
Elastic Modulus ◽  
Osteogenic Differentiation ◽  
Cell Morphology ◽  
Subcellular Location ◽  
Substrate Stiffness ◽  
Matrix Stiffness ◽  
Osteogenic Markers ◽  
Enhanced Expression ◽  
Extracellular Matrix Stiffness ◽  
Cells Cultured

Osteogenic differentiation of cells has considerable clinical significance in bone defect treatment, and cell behavior is linked to extracellular matrix stiffness. This study aimed to determine how matrix stiffness affects cell morphology and subsequently regulates the osteogenic phenotype of osteogenesis precursor cells. Four PDMS substrates were prepared with stiffness corresponding to the elastic modulus ranging from 0.6 MPa to 2.7 MPa by altering the Sylgard 527 and Sylgard 184 concentrations. MC3T3-E1 cells were cultured on the matrices. Cell morphology, vinculin expression, and key osteogenic markers, Col I, OCN, OPN, and calcium nodule, were examined. The activity and expression level of Yes-associated protein (YAP) were evaluated. Results showed that cell spreading exhibited no correlation with the stiffness of matrix designed in this paper, but substratum stiffness did modulate MC3T3-E1 osteogenic differentiation. Col I, OPN, and OCN proteins were significantly increased in cells cultured on soft matrices compared with stiff matrices. Additionally, cells cultured on the 1:3 ratio matrices had more nodules than those on other matrices. Accordingly, cells on substrates with low stiffness showed enhanced expression of the osteogenic markers. Meanwhile, YAP expression was downregulated on soft substrates although the subcellular location was not affected. Our results provide evidence that matrix stiffness (elastic modulus ranging from 0.6 MPa to 2.7 MPa) affects the osteogenic differentiation of MC3T3-E1, but it is not that “the stiffer, the better” as showed in some of the previous studies. The optimal substrate stiffness may exist to promote osteoblast differentiation. Cell differentiation triggered by the changes in substrate stiffness may be independent of the YAP signal. This study has important implications for biomaterial design and stem cell-based tissue engineering.

scholarly journals Integrated metabolic profiling and transcriptome analysis of pigment accumulation in diverse petal tissues in the lily cultivar ‘Vivian’

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Xiaojuan Yin ◽  
Xinyue Lin ◽  
Yuxuan Liu ◽  
Muhammad Irfan ◽  
Lijing Chen ◽  
...  
Keyword(s):  
Cell Morphology ◽  
Ornamental Plants ◽  
Flower Color ◽  
Rna Seq ◽  
Regulation Network ◽  
High Level ◽  
Relationship Of ◽  
The Relationship ◽  
Pigment Accumulation ◽  
Related Metabolite

Abstract Background Petals are the colorful region of many ornamental plants. Quality traits of petal color directly affect the value of ornamental plants. Although the regulatory mechanism of flower color has been widely studied in many plants, that of lily flower color is still worth further exploration. Results In this study, the pigmentation regulatory network in different regions of the petal of lily cultivar ‘Vivian’ was analyzed through tissue structure, metabolites biosynthesis, and gene expression. We found that cell morphology of the petal in un-pigmented region differed from that in pigmented region. The cell morphology tends to flatten in un-pigmented region where the color is lighter. Moreover, high level anthocyanin was found in the pigmented regions by metabonomic analysis, especially cyanidin derivatives. However, flavanones were accumulated, contrast with anthocyanin in the un-pigmented regions of lily petal. To understand the relationship of these different metabolites and lily flower color, RNA-Seq was used to analyze the differentially expressed genes-related metabolite biosynthesis. Among these genes, the expression levels of several genes-related cyanidin derivatives biosynthesis were significantly different between the pigmented and un-pigmented regions, such as LvMYB5, LvMYB7, LvF3’H, LvDFR, LvANS and Lv3GT. Conclusions This data will help us to further understand the regulation network of lily petal pigmentation and create different unique color species.

scholarly journals OC-157 Physiological changes in matrix stiffness modulate hepatic progenitor cell morphology, proliferation and differentiation

Gut ◽  
2012 ◽  
Vol 61 (Suppl 2) ◽  
pp. A67.3-A68 ◽  
Author(s):  
T T Gordon-Walker ◽  
J Schrader ◽  
L Boulter ◽  
A J Robson ◽  
S J Forbes ◽  
...  
Keyword(s):  
Cell Morphology ◽  
Progenitor Cell ◽  
Physiological Changes ◽  
Matrix Stiffness ◽  
Hepatic Progenitor Cell ◽  
Proliferation And Differentiation

Double IgA bands in serum from a patient with lymphoplasmacytoid leukemia with hairy-cell morphology.

1987 ◽  
Vol 33 (12) ◽  
pp. 2317-2319 ◽  
Author(s):  
S Y Loo ◽  
N V Bhagavan ◽  
A G Scottolini
Keyword(s):  
B Cell ◽  
Cell Morphology ◽  
Recurrent Disease ◽  
Lymphocytic Leukemia ◽  
Hairy Cell ◽  
Response To Chemotherapy ◽  
Relationship Of ◽  
The Relationship

Abstract We present a case of plasmacytoid lymphocytic leukemia with hairy-cell-like cytoplasmic projections and separate monomeric and polymeric IgA(lambda) serum bands confirmed by immunofixation. After a prolonged initial good response to chemotherapy, the patient had recurrent disease with increased plasmacytoid blastic feature and died. The relationship of this case to B-cell proliferative disorders is discussed.

scholarly journals Integrated Metabolic Profiling and Transcriptome Analysis of Pigment Accumulation in Diverse Petal Tissues in the Lily Cultivar 'Vivian'

2020 ◽  
Author(s):  
Xiaojuan Yin ◽  
Xinyue Lin ◽  
Yuxuan Liu ◽  
Muhammad Irfan ◽  
Lijing Chen ◽  
...  
Keyword(s):  
Cell Morphology ◽  
Ornamental Plants ◽  
Flower Color ◽  
Rna Seq ◽  
Regulation Network ◽  
High Level ◽  
Relationship Of ◽  
The Relationship ◽  
Pigment Accumulation ◽  
Related Metabolite

Abstract Background: Petals are the colorful region of many ornamental plants. Quality traits of petal color directly affect the value of ornamental plants. Although the regulatory mechanism of flower color has been widely studied in many plants, that of lily flower color is still worth further exploration.Results: In this study, the pigmentation regulatory network in different regions of the petal of lily cultivar ‘Vivian’ was analyzed through tissue structure, metabolites biosynthesis, and gene expression. We found that cell morphology of the petal in un-pigmented region was differed with in pigmented region. The cell morphology tends to flatten in un-pigmented region where the color is lighter. Moreover, high level anthocyanin was found in the pigmented regions by metabonomic analysis, especially cyanidin derivatives. However, flavanones were accumulated, contrast with anthocyanin in the un-pigmented regions of lily petal. To understand the relationship of these different metabolites and lily flower color, RNA-Seq was used to analyze the differentially expressed genes-related metabolite biosynthesis. Among these genes, the expression levels of several genes-related cyanidin derivatives biosynthesis were significantly different between the pigmented and un-pigmented regions, such as LvMYB5, LvMYB7, LvF3'H, LvDFR, LvANS and Lv3GT.Conclusions: This data will help us to further understand the regulation network of lily petal pigmentation and create different unique color species.

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